Abstract
Hypocotyl explants of 1 and 10 mm lengths were excised from 12-day-old in vitro-grown seedlings of Albizia richardiana. The larger pieces, after 40 days of culture, developed shoots along with green calli on B5 + BAP (10−7–10−5M), while the smaller segments produced only green calli on B5+BAP (10−7–10−4M) medium. Some of the green calli turned morphogenic and started producing somatic embryos with the 2nd sub-culture and shoots from 7th sub-culture onwards. Calli retained the morphogenic potential even after repeated sub-culturing for over two years. The number of embryos in an embryogenic culture varied from 2 to 20 per callus mass of 5–6.5 cm3. Sucrose at the 2% level in MS medium was optimal for embryogenesis while 4% was optimal for shoot bud differentiation. Higher levels of sucrose (6–10%) caused browning of green calli and also inhibited differentiation into embryos and shoot buds. By selective sub-culturing of 0.1 cm3 pieces of embryogenic calli on MS+10−5M BAP, 46% of the cultures produced somatic embryos. The latter germinated into plantlets on Knop's medium.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- B5 :
-
Gamborg et al., 1968 medium
- IAA:
-
Indole-3-acetic acid
- MS:
-
Murashige and Skoog's (1962) medium
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Communicated by J. M. Widholm
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Tomar, U.K., Gupta, S.C. Somatic embryogenesis and organogenesis in callus cultures of a tree legume — Albizia richardiana King. Plant Cell Reports 7, 70–73 (1988). https://doi.org/10.1007/BF00272982
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DOI: https://doi.org/10.1007/BF00272982