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Interrelationships Between miR-34a and FSH in the Control of Porcine Ovarian Cell Functions

  • Reproductive Biology: Original Article
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Abstract

Our study aimed to elucidate the effect of miR-34a mimics and miR-34a inhibitor and their combination on basic functions of ovarian cells cultured with and without FSH, and effect of FSH on expression of endogenous miR-34a. Viability, proliferation, proportion of proliferative active cells, apoptosis, proportion of DNA fragmented cells, accumulation of FSHR, steroid hormones, IGF-I, oxytocin, and prostaglandin E2 release, and expression levels of miR-34a were analysed. MiR-34a mimics decreased proliferation, apoptosis, testosterone, and estradiol output, stimulated release of progesterone, IGF-I, oxytocin, and occurrence of FSHR. MiR-34a inhibitor had an opposite effect and prevented effects of miR-34a mimics. FSH promoted expression of miR-34a, viability, proliferation, steroid hormones, IGF-I, oxytocin, and prostaglandin E2 output, and reduced apoptosis. Furthermore, miR-34a mimics supported effect of FSH on viability, apoptosis, progesterone, and IGF-I and inverted FSH action on proliferation, testosterone, and estradiol output. Our observations suggest that miR-34a can be involved in control of basic ovarian functions and that miR-34a and FSH are synergists in their actions on ovarian cell functions. Ability of FSH to promote miR-34a expression and ability of miR-34a mimics to increase occurrence of FSHR and to modify FSH effects suggest the existence of self-stimulating FSH-miR-34a axis, and that miR-34a can mediate actions of FSH on ovarian cells.

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Funding

This research was financially supported by the Slovak Research and Development Agency (APVV), the project APVV-15-0296 and the Scientific Grant Agency of the Ministry of Education, Science, and Sport of Slovak Republic (VEGA), the projects VEGA 13-ENV1321-02 and 1/0680/22.

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  1. Department of Zoology and Anthropology, Constantine the Philosopher University, Tr. A. Hlinku 1, 949 74, Nitra, Slovakia

    Zuzana Fabová, Barbora Loncová, Miroslav Bauer & Alexander V. Sirotkin

  2. NPPC, Research Institute for Animal Production Nitra, Hlohovecká 2, 951 41, Lužianky, Slovakia

    Miroslav Bauer

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  1. Zuzana Fabová
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Fabová, Z., Loncová, B., Bauer, M. et al. Interrelationships Between miR-34a and FSH in the Control of Porcine Ovarian Cell Functions. Reprod. Sci. 30, 1789–1807 (2023). https://doi.org/10.1007/s43032-022-01127-2

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