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Biopolishing of denim by the recombinant xylanase II of Caulobacter crescentus

  • Biotechnology and Industrial Microbiology - Research Paper
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Abstract

Denim, also known as jeans, is a fabric made up of braided cotton threads dyed indigo blue, whose fibers contain approximately 10% of non-cellulosic impurities that reduce its commercial value. Microbial enzymes can act in the cleaning and desizing processes of jeans, improving their color, softness, and covering capacity. The recombinant Xylanase II (XynA2) from the aquatic bacterial Caulobacter crescentus (C. crescentus), previously characterized in terms of its biochemical features, was applied to the biotreatment of jeans to clean and degum it. The biotreatment performance was evaluated in terms of tissue weight loss, amount of reducing sugars released and analysis of the images obtained by scanning electron microscopy (SEM). Biotreated tissues, at 12 and 24 h, showed a dry weight loss of 4.9 and 6.6%, respectively. The reducing sugars amount released after XynA2 action over the jean’s fibers showed statistically significant values when compared with each other and with their respective controls. SEM images clearly shown that the fabric treated for 12 h presented a smooth and polished surface, while the fabric treated for 24 h showed the cotton fibers broken, displaying severe damage to the textile. The best treatment for the jeans was in the presence of 1 U mg-1 XynA2 at pH 8 and 60 °C during 12 h. In conclusion, XynA2 of C. crescentus was satisfactorily applied for the biopolishing of denim jeans being a more sustainable alternative to the use of chemical and abrasive processes to obtain the same effects.

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The authors declare that the data obtained in this work are available to anyone interested and statement that all scientific data published will be used exclusively for the services provided by this publication, not being available for other purposes or to third parties.

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Acknowledgements

The authors thank Coordination of Improvement of Higher Education Personnel (CAPES).

Funding

Despite the drastic reduction of investments in research by the Brazilian government in recent years, this project received the following support: D. Jacomini and L. Bussler were fellow of CAPES (Coordination of Improvement of Higher Education Personnel of Brazil).

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Authors and Affiliations

  1. Centro de Ciências Médicas e Farmacêuticas - CCMF, Laboratório de Bioquímica Molecular – LaBioqMol, Universidade Estadual do Oeste do Paraná- UNIOESTE, Rua Universitária, 2069 -, Cascavel, PR, 85814-110, Brazil

    Débora Jacomini, José Luis da-Conceição Silva, Alexandre Maller, Marina Kimiko Kadowaki & Rita de Cássia Garcia Simão

  2. Centro de Ciências Exatas e Tecnológicas, Laboratório de Bioquímica Molecular– LaBioqMol, Universidade Estadual do Oeste do Paraná, Paraná, Cascavel, Brazil

    Larissa Bussler

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  1. Débora Jacomini
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  2. Larissa Bussler
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  3. José Luis da-Conceição Silva
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  4. Alexandre Maller
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  5. Marina Kimiko Kadowaki
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  6. Rita de Cássia Garcia Simão
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Correspondence to Rita de Cássia Garcia Simão.

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Débora Jacomini and Larissa Bussler contributed equally to this work.

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Jacomini, D., Bussler, L., da-Conceição Silva, J. et al. Biopolishing of denim by the recombinant xylanase II of Caulobacter crescentus. Braz J Microbiol 54, 1559–1564 (2023). https://doi.org/10.1007/s42770-023-01056-5

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  • DOI: https://doi.org/10.1007/s42770-023-01056-5

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