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Application of Thermostable Xylanase of Bacillus pumilus in Textile Processing

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Abstract

Desizing of cotton and micropoly fabrics was done using thermostable xylanase from Bacillus pumilus ASH. Micropoly fabric showed better desizing than cotton under same conditions. Violet scale readings from the TEGEWA test after enzymatic desizing for 90 min at pH 7.0 and at 60°C showed the readings falling in the range of 4–5, indicating good desizing efficiency. During bioscouring the weight loss values and liberation of reducing sugars were highest when EDTA was used along with xylanase. The weight loss value of 1.5% was observed for dry cotton fabric after 1 h in case of agitated system at pH 7.0 and at an optimal enzyme dosage of 5 IU/g. The weight loss values and the liberation of reducing sugars were higher in case of cotton fabrics. Wetting time of fabrics was lowered significantly after 60 min of bioscouring using xylanase. Increase in temperature or concentration of surfactant led to further reduction in the wetting time. The whiteness values of fabrics after bioscouring were 0.9% higher than the chemically scoured fabrics indicating good efficacy of xylanase during the scouring process.

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References

  1. Sunna A, Antranikian G (1997) Xylanolytic enzymes from fungi and bacteria. Crit Rev Biotechnol 17:39–67

    Article  PubMed  CAS  Google Scholar 

  2. Gilbert HJ, Hazlewood GP (1999) Bacterial cellulases and xylanase. J Gen Microbiol 139:187–194

    Google Scholar 

  3. Battan B, Sharma JK, Kuhad RC (2006) High-level xylanase production by alkalophilic Bacillus pumilus ASH under solid-state fermentation. World J Microbiol Biotechnol 22:1281–1287

    Article  CAS  Google Scholar 

  4. Ball AS, McCarthy AJ (1989) Saccharification of straw by actinomycete enzyme. J Appl Bacteriol 66:439–444

    Article  CAS  Google Scholar 

  5. Hrmova M, Biely P, Vrzanka M, Petrakova E (1984) Induction of cellulose and xylan degrading enzyme complex in yeast Trichoderma cutaneum. Arch Microbiol 161:371–376

    Article  Google Scholar 

  6. Liu W, Zhu W, Lu Y, Kong Y, Ma G (1998) Production, partial purification and characterization of xylanase from Trichosporon cutaneum SL409. Process Biochem 33:331–336

    Article  CAS  Google Scholar 

  7. Lipp-Symonowicz B, Tanska B, Wolukanis A, Wrzosek H (2004) Influence of enzymatic treatment on the flax fibre morphological structure, physico-chemical properties and metrological parameters of yarn. Fibres Text 12:61–65

    CAS  Google Scholar 

  8. Rouette HK (2001) Encyclopedia of textile finishing. Springer, Berlin, pp 1–3, ISBN 3-540-65031-8

  9. Karmakar SR (1999) Chemical technology in the pretreatment processes of textiles. In: Textile science and technology series, 1st edn. Elsevier Science B.V., Amsterdam, p 12

    Google Scholar 

  10. Hartzell MM, Hsieh YL (1998) Enzymatic scouring to improve cotton fabric wettability. Text Res J 68(4):233–241

    Article  CAS  Google Scholar 

  11. Li Y, Hardin IR (1998) Enzymatic scouring of cotton-surfactants, agitation and selection of enzymes. Text Chem Color 30:23–29

    CAS  Google Scholar 

  12. Etters JN (1999) Cotton preparation with alkaline pectinase: an environmental advance. Text Chem Color Am Dyestuff Rep 1(3):33–36

    CAS  Google Scholar 

  13. Traore MK, Buschle-Diller G (1999) Environmentally friendly scouring processes. In: Book of papers of the international conference and exhibition of the AATCC, Charlotte, pp 183–189

  14. Buchert J, Pere J, Puolakka A, Nousiainen P (2000) Scouring cotton with pectinases, proteases and lipases. Text Chem Color Am Dyestuff Rep 32(5):48–52

    CAS  Google Scholar 

  15. Csiszar E, Urbanskzi K, Szakaes G (2001) Biotreatment of desized cotton fabric by commercial cellulase and xylanase enzymes. J Mol Catal B 11:1065–1072

    Article  CAS  Google Scholar 

  16. Yachmenev VG, Bertoniere NR, Blanchard EJ (2001) Effect of sonification on cotton preparation with alkaline pectinase. Text Res J 71(6):527–533

    Article  CAS  Google Scholar 

  17. Lenting HBM, Zwier E, Nierstrasz VA (2002) Identifying Important Parameters for a Continuous Bioscouring Process. Text Res J 72(9):825–831

    Article  CAS  Google Scholar 

  18. Agrawal PB, Nierstrasz VA, Warmoeskerken MMCG (2004) Enhanced Bioscouring Performance. In: Proceedings of the 4th Autex conference, Roubaix, France, 22–24 June, pp 165–173

  19. Lenting HBM, Warmoeskerken MMCG (2004) A fast, continuous enzyme-based pretreatment process concept for cotton containing textiles. Biocatal Biotransform 22(5/6):361–368

    Article  CAS  Google Scholar 

  20. Li Y, Hardin IR (1997) Enzymatic scouring of cotton: effects on structure and properties. Text Chem Color 29:71–76

    CAS  Google Scholar 

  21. Buchert J, Pere J, Puolakka A, Nousiainen P (1998) Enzymatic scouring of cotton. In: Book of papers, AATCC international conference and exhibition 1998, Philadelphia. Am Assoc Text Chem Color, 493–499

  22. Losonczi A, Csiszar E, Szakacs G, Bezur L (2005) Role of the EDTA chelating agent in bioscouring of cotton. Text Res J 75(5):411–417

    Article  CAS  Google Scholar 

  23. Verschraege L (1989) Cotton fibre impurities. Neps, motes and seed coat fragments. ICAC review articles on cotton production research No 1. CAB International, Wallingford

  24. Csiszar E, Losonczi A, Szakacs G, Rusznak I, Bezur L, Reicher J (2001) Enzymes and chelating agents in cotton pretreatment. J Biotechnol 89(2–3):271–279

    Article  PubMed  CAS  Google Scholar 

  25. Battan B, Sharma J, Dhiman SS, Kuhad RC (2007) Enhanced production of cellulase-free thermostable xylanase by Bacillus pumilus ASH and its potential application in paper industry. Enzyme Microb Technol 41:733–739

    Article  CAS  Google Scholar 

  26. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  27. Hartzell-Lawson MM, Hsieh Y-L (2000) Characterizing the non cellulosics in developing cotton fibres. Text Res J 70(9):810–819

    Article  CAS  Google Scholar 

  28. Sindhu I, Chhibber S, Capalash N, Sharma P (2006) Production of cellulase-free xylanase from Bacillus megaterium by solid state fermentation for biobleaching of pulp. Curr Microbiol 53:167–172

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors gratefully acknowledge Nahar Group of Industries, Punjab, India for providing cotton and micropoly fabrics and the laboratory facilities. Bindu Battan greatly acknowledges the financial assistance from Council of Scientific and Industrial Research, India in the form of Senior Research Fellowship during the course of investigation. Saurabh Sudha Dhiman and Sonia Ahlawat wish to thank Kurukshetra University, Kurukshetra for University Research Scholarship.

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Correspondence to Ritu Mahajan.

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Battan, B., Dhiman, S.S., Ahlawat, S. et al. Application of Thermostable Xylanase of Bacillus pumilus in Textile Processing. Indian J Microbiol 52, 222–229 (2012). https://doi.org/10.1007/s12088-011-0118-1

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  • DOI: https://doi.org/10.1007/s12088-011-0118-1

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