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Living Electrospun Short Fibrous Sponge via Engineered Nanofat for Wound Healing

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Abstract

Living cells and active factors are the two core elements of tissue repair, directly affecting the healing efficiency of damaged tissue. Nanofat (NF) can release living cells, such as adipose-derived stem cells (ADSCs), as well as active growth factors to promote angiogenesis, thus realizing cell-based wound healing. Herein, a novel living electrospun short fibrous sponge is constructed by modifying three-dimensional (3D) bionic short fibers with engineered NF. The uniform distribution of the polydopamine (PDA) modification endows the living sponges with stable mechanical properties, reversible water absorption and excellent adhesion even after repeated compression by an external force and long-term aqueous immersion. Meanwhile, the living electrospun short fibrous sponges with uniform NF modification contain living cells such as ADSCs and active growth factors such as vascular endothelial growth factor (VEGF), which can effectively promote the tube formation of human umbilical vein endothelial cells (HUVECs). In vivo, the living sponges can effectively and continuously act on wounds and act as a bionic living skin to prevent the loss of internal nutrients, creating a comfortable and favorable microenvironment for tissue regeneration and promoting the healing of diabetic wounds. Therefore, living electrospun short fibrous sponges via engineered NF are expected to achieve continuous wound healing with in situ living cells and active factors in injured tissues.

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Acknowledgements

X. F and J. W contributed equally to this work. This research was funded by the National Key Research and Development Program of China (2020YFA0908200), National Natural Science Foundation of China General Program (32000937 and 51873107), Shanghai Municipal Health Commission (20204Y0354), Youth Innovation Technology Project of Higher School in Shandong Province (20190919), China Postdoctoral Science Foundation (2022T150426) and Program of Shanghai Academic Research Leader (22XD1422600).

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  1. Xiaohan Fu and Juan Wang contributed equally to the work.

Authors and Affiliations

  1. Department of Plastic Surgery, Key Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Shandong Provincial Maternal and Child Health Care Hospital Affiliated to Qingdao University, Jinan, 250014, People’s Republic of China

    Xiaohan Fu, Juan Wang & Yan Wang

  2. Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, People’s Republic of China

    Xiaohan Fu, Juan Wang, Liang Chen & Wenguo Cui

  3. Department of Orthopedic Surgery, The First Affliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, 250014, People’s Republic of China

    Dejian Qian

  4. Department of Obstetric, Shandong Provincial Maternal and Child Health Care Hospital Affiliated to Qingdao University, Jinan, 250014, People’s Republic of China

    Zhaowen Chen

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  1. Xiaohan Fu
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Fu, X., Wang, J., Qian, D. et al. Living Electrospun Short Fibrous Sponge via Engineered Nanofat for Wound Healing. Adv. Fiber Mater. 5, 979–993 (2023). https://doi.org/10.1007/s42765-022-00229-5

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