Abstract
Biochar (BC) is a promising soil amendment for mitigating nitrous oxide (N2O) emissions. However, field experiments have reported inconsistencies in the changes in N2O emissions, and the underlying microbial mechanisms are unclear. A tobacco (Nicotiana tabacum L.) field under different tobacco BC application rates (0, 1, 10, 25, and 50 t ha−1) was established to investigate the changes of soil N2O emissions and microbial community compositions. BC amendments significantly increased the cumulative N2O emissions by 1.96–4.18 folds, mainly due to enhanced soil substrate availability under tobacco BC application. Shifts of bacterial community structure at the phylum level under BC amendment were observed, while changes in the structure of soil fungi at the genus level occurred. The abundance of denitrifying bacteria (Bradyrhizobium and Pseudomonas) and denitrifying fungi (Trichocladium and Trichoderma) was significantly increased with BC amendment, contributing to the stimulated soil N2O emissions by affecting aerobic denitrification. The field N2O mitigation of BC application should be reconsidered if tobacco BC is applied to upland soils.
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Data Availability
The raw data sequences used in this study are freely accessible via NCBI (https://www.ncbi.nlm.nih.gov/) with accession number SAMN29594028-SAMN29594042. This repository provides open access and long-term digital preservation of all data.
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Funding
This study was funded by the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDB40020201), the National Natural Science Foundation of China (42263013), the Science and Technology Program of Guizhou Province (Grant Nos. [2021]187 and ZK[2022]047), the Science and Technology Project of the Guizhou Company of the China Tobacco Corporation (Grant No. 2020XM08), the Key Research and Development Program of the China Tobacco Corporation (Grant No. 110202102038), and the Opening Fund of the State Key Laboratory of Environmental Geochemistry (SKLEG2023212). Jianzhong Cheng was supported by the “Light of West China” Program of Chinese Academy of Sciences.
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Tang, Y., Gao, W., Chen, Y. et al. Effects of Biochar Amendment on Nitrous Oxide Emission, Bacterial and Fungal Community Composition in a Tobacco-Planting Soil. J Soil Sci Plant Nutr 23, 3106–3119 (2023). https://doi.org/10.1007/s42729-023-01288-2
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DOI: https://doi.org/10.1007/s42729-023-01288-2