Abstract
Integrated signaling network involving abscisic acid (ABA), nitric oxide (NO), and indole-acetic acid (IAA) controls root morphogenesis during salt stress by a mechanism still poorly understood. The present data unveiled an ABA-NO-IAA interaction underlying radicular morphological responses to salinity. Three Solanum lycopersicum genotypes were analyzed: wild type, ABA-insensitive mutant (sitiens), and auxin-responsive (DR5::GUS) plants. Nitric oxide fluorescence, nitrate reductase activity, auxin signaling, and some molecular analyses were performed. Pharmacological inhibitors and NO donor sodium nitroprusside were also used to evaluate NaCl-induced root morphological responses. Sodium nitroprusside inhibited primary root length, increased lateral root emergence, and rescued salt inhibited lateral root growth. The results showed that NO integrates the ABA-IAA signaling network of root system responses under salt stress, involving: (a) ABA and molybdenum-dependent enzymes as responsible for salt-induced NO production; (b) modulations of the plasma membrane H+-ATPase coupling and isoforms differential expression; and (c) ABA-mediated and NO-dependent antioxidative enzymes activities.
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The authors would like to acknowledge the Brazilian National Council for Scientific and Technological Development (CNPq) and Coordination for the Improvement of Higher Education Personnel (CAPES) for financial support.
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MPS designed and conceived the experiment, performed the molecular analysis, analyzed data and wrote the paper. DBZ performed molecular analysis and reviewed the paper. AFLS Performed the data analysis. EPC performed molecular analysis. CJLO, LEPP, ARF and RBS reviewed the paper. All the authors reviewed and approved the final version of the manuscript.
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Santos, M.P., Zandonadi, D.B., de Sá, A.F.L. et al. Abscisic acid-nitric oxide and auxin interaction modulates salt stress response in tomato roots. Theor. Exp. Plant Physiol. 32, 301–313 (2020). https://doi.org/10.1007/s40626-020-00187-6
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DOI: https://doi.org/10.1007/s40626-020-00187-6