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Gene expression analysis reveals diversified responsiveness to salt stress in rice genotypes

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Abstract

Salt stress is a major factor affecting rice (Oryza sativa) growth and productivity, limiting its distribution globally. Rice production is primarily gets affected due to its vulnerability to salinity stress at seedling stage, as well as reproductive stage leading to reduction in yield. We report the analysis of 12 diverse rice genotypes collected from different parts of coastal places of northern Andra Pradesh and southern Orissa in India for salt tolerance and categorized their tolerance levels on the basis of reduction in growth and transcript expression pattern. The analysis identified four contrasting salt tolerant genotypes and other eight genotypes exhibited moderate tolerance. Two tolerant and two sensitive genotypes were biochemically explored and used further for gene expression analysis. Proline content showed significant difference between salt tolerant and sensitive genotypes suggesting high tolerance level in tolerant CR1014 and Kudrat-5. The gene-expression analysis of selected CR1014 (salt-tolerant) and Kudrat-5 (salt-sensitive) genotypes differed at transcript expression levels for antioxidant specific A2YPX2/plant peroxidase—POX (19.03-fold) and SODCP/superoxide dismutase (SOD) copper/zinc binding (3.77-fold) in shoots of Kudrat-5 and CATA1/catalase (2.49-fold) and SODCP/SOD copper/zinc binding (4.83-fold) in CR1014 in stress conditions). The salt-stress responsive-specific transcripts HAK17/K+ potassium transporter, A2XMP7/heat shock protein DnaJ, cysteine-rich domain and Q10D68/serine hydroxymethyltransferase in CR1014 after treatment showed increased expression. Interestingly, the transcripts specific for methionine biosynthesis mainly A2Z9C9/Cys/Met metabolism, pyridoxal phosphate-dependent enzyme and B8AF89/spermine synthase induced their expression in Kudrat-5 under salinity stress, where the similar trend was not reflected in salt-tolerant genotype. Thus, the present study highlights on transcript expression pattern for salt-stress responsive candidate genes in two-contrasting rice genotypes selected from the pool of farmer’s cultivated varieties along the coastal belts of India.

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References

  • Ashraf, M., & Harris, P. J. C. (2004). Potential biochemical indicators of salinity tolerance in plants. Plant Science, 166, 3–16. https://doi.org/10.1016/j.plantsci.2003.10.024.

    Article  CAS  Google Scholar 

  • Bao, J. S., Cai, Y., Sun, M., Wang, G., & Corke, H. (2005). Anthocyanins, flavonols, and free radical scavenging activity of Chinese bayberry (Myrica rubra) extracts and their color properties and stability. Journal Agricultural and Food Chemistry, 53, 2327–2332.

    Article  CAS  Google Scholar 

  • Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205–208.

    CAS  Google Scholar 

  • Ben Ahmed, C., Magdich, S., Rouina, B. B., Sensoy, B. M., & Abdullah, F. B. (2011). Exogenous proline effects on water relations and ions concentrations in leaves and roots of young olive. Amino Acids, 40, 565–573.

    Article  CAS  Google Scholar 

  • Bevilacqua, C. B., Basu, S., Pereira, A., Tseng, T. M., Zimmer, P. D., & Burgos, N. R. (2015). Analysis of stress-responsive gene expression in cultivated and weedy rice differing in cold stress tolerance. PLoS ONE, 10, e0132100.

    Article  Google Scholar 

  • Cassaniti, C., Leonardi, C., & Flowers, T. J. (2009). The effects of sodium chloride ornamental shrubs. Scientia Horticulturae, 122, 586–593.

    Article  CAS  Google Scholar 

  • Cassaniti, C., Romano, D., & Flowers, T. J. (2012). The response of ornamental plants to saline irrigation water. In I. Garcia-Garizabal (Ed.), Irrigation water management, pollution and alternative strategies (pp. 132–158). Rijeka: InTech Europe.

    Google Scholar 

  • Dubouzet, J. G., Sakuma, Y., Ito, Y., Kasuga, M., Dubouzet, E. G., Miura, S., et al. (2003). OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold responsive gene expression. Plant Journal, 33, 751–763.

    Article  CAS  Google Scholar 

  • Fernie, A. R., Geigenberger, P., & Stitt, M. (2005). Flux an important, but neglected, component of functional genomics. Current Opinion in Plant Biology, 8, 174–182.

    Article  CAS  Google Scholar 

  • Gregorio, G. B. (1997). Ph.D. Thesis, University of the Philippines Los Baños. Laguna, Philippines. http://agris.fao.org/agris-search/search.do?recordID=PH1998010269

  • Hasanuzzaman, M., Alam, M. M., Rahman, A., Hasanuzzaman, M., Nahar, K., Fujita M. (2014). Exogenous proline and glycine betaine mediated upregulation of antioxidant defense and glyoxalase systems provides better protection against salt-induced oxidative stress in two rice (Oryza sativa L.) varieties. Biomed Research International-Hindawi, 2014, 1–17

    Google Scholar 

  • Hasegawa, P. M., Bressan, R. A., Zhu, J. K., & Bohnert, H. J. (2000). Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology, 51, 463–499. https://doi.org/10.1146/annurev.arplant.51.1.463.

    Article  CAS  PubMed  Google Scholar 

  • Hsiao, T. C. & Xu, L. K. (2000). Sensitivity of growth of roots versus leaves to water stress: biophysical analysis and relation to water transport. Journal of Experimental Botany, 51, 1595–1616.

    Article  CAS  Google Scholar 

  • Kibria, M. G., Hossain, M., Murata, Y., & Hoque, Md A. (2017). Antioxidant defense mechanisms of salinity tolerance in rice genotypes. Rice Science, 24(3), 155–162.

    Article  Google Scholar 

  • Kumar, V., Shriram, V., Kavi Kishor, P. B., Jawali, N., & Shitole, M. G. (2010). Enhanced proline accumulation and salt stress tolerance of transgenic indica rice by over-expressing P5CSF129A gene. Plant Biotechnology Reports, 4(1), 37–48.

    Article  Google Scholar 

  • Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(− ΔΔC(T)) method. Methods, 25, 402–408.

    Article  CAS  Google Scholar 

  • Maggio, A., Miyazaki, S., Veronese, P., Fujita, T., Ibeas, J., Damsz, B., et al. (2002). Does proline accumulation play an active role in stress-induced growth reduction? The Plant Journal, 31, 699–712.

    Article  CAS  Google Scholar 

  • Morsy, M. R., Almutairi, A. M., Gibbons, J., Yun, S. J., & de Los Reyes, B. G. (2005). The OsLti6 genes encoding low-molecular-weight membrane proteins are differentially expressed in rice cultivars with contrasting sensitivity to low temperature. Gene, 344, 171–180.

    Article  CAS  Google Scholar 

  • Munns, R., & Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651–681.

    Article  CAS  Google Scholar 

  • Ogawa, S. & Mitsuya, S. (2012). S-methylmethionine is involved in the salinity tolerance of Arabidopsis thaliana plants at germination and early growth stages. Physiologia Plantarum, 144(1), 13–19.

    Article  CAS  Google Scholar 

  • Rahneshan, Z., Nasibi, F., & Moghadam, A. A. (2018). Effects of salinity stress on some growth, physiological, biochemical parameters and nutrients in two pistachio (Pistacia vera L.) rootstocks. Plant-Environment Interactions, Journal of Plant Interactions, 13(1), 73–82.

    Article  Google Scholar 

  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231–1237.

    Article  CAS  Google Scholar 

  • Sahi, C., Singh, A., Blumwald, E., & Grover, A. (2006). Beyond osmolytes and transporters: Novel plant salt-stress tolerance-related genes from transcriptional profiling data. Physiologia Plantarum, 127, 1–9.

    Article  CAS  Google Scholar 

  • Sharma, R., Mishra, M., Gupta, B., Parsania, C., Singla-Pareek, S. L., & Pareek, A. (2015). De novo assembly and characterization of stress transcriptome in a salinity-tolerant variety CS52 of Brassica juncea. PLoS ONE, 10(5), e0126783.

    Article  Google Scholar 

  • Wang, D., Liu, H., Li, S., Zhai, G., Shao, J., & Tao, Y. (2015). Characterization and molecular cloning of a serine hydroxymethyltransferase 1 (OsSHM1) in rice. Journal of Integrated Plant Biology, 57(9), 745–756. https://doi.org/10.1111/jipb.12336.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology and Crop Physiology, M. S. Swaminathan School of Agriculture, Centurion University, Paralakhemundi, Orissa, 761211, India

    G. Pushpalatha

  2. Department of Genetics and Plant Breeding, M. S. Swaminathan School of Agriculture, Centurion University, Paralakhemundi, Orissa, 761211, India

    G. Harish Kumar

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  1. G. Pushpalatha
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Correspondence to G. Pushpalatha.

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Conflict of interest

Conceived and designed the experiments: GPL, Performance of the experiments: GHK, Analysed and wrote the paper: GPL and HKG. The authors GPL and HKG declare that there is no conflict of interests regarding publishing this paper.

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Pushpalatha, G., Harish Kumar, G. Gene expression analysis reveals diversified responsiveness to salt stress in rice genotypes. Ind J Plant Physiol. 23, 833–843 (2018). https://doi.org/10.1007/s40502-018-0424-2

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