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Enhanced proline accumulation and salt stress tolerance of transgenic indica rice by over-expressing P5CSF129A gene

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Abstract

Δ1-pyrroline-5-carboxylate synthetase (P5CS) is a proline biosynthetic pathway enzyme and is known for conferring enhanced salt and drought stress in transgenics carrying this gene in a variety of plant species; however, the wild-type P5CS is subjected to feedback control. Therefore, in the present study, we used a mutagenized version of this osmoregulatory gene-P5CSF129A, which is not subjected to feedback control, for producing transgenic indica rice plants of cultivar Karjat-3 via Agrobacterium tumefaciens. We have used two types of explants for this purpose, namely mature embryo-derived callus and shoot apices. Various parameters for transformation were optimized including antibiotic concentration for selection, duration of cocultivation, addition of phenolic compound, and bacterial culture density. The resultant primary transgenic plants showed more enhanced proline accumulation than their non-transformed counterparts. This proline level was particularly enhanced in the transgenic plants of next generation (T1) under 150 mM NaCl stress. The higher proline level shown by transgenic plants was associated with better biomass production and growth performance under salt stress and lower extent of lipid peroxidation, indicating that overproduction of proline may have a role in counteracting the negative effect of salt stress and higher maintenance of cellular integrity and basic physiological processes under stress.

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Acknowledgments

This work was supported by a grant from Board of Research in Nuclear Sciences, Department of Atomic Energy (DAE-BRNS), Government of India (Grant No. 2004/37/28/BRNS) to M.G. Shitole and a research fellowship to the first author.

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  1. Vinay Kumar

    Present address: Department of Biotechnology, Modern College of Arts, Science and Commerce, Ganeshkhind, Pune, 411 053, India

Authors and Affiliations

  1. Department of Botany, University of Pune, Ganeshkhind, Pune, 411 007, India

    Vinay Kumar & M. G. Shitole

  2. Department of Botany, Annasaheb Magar College, Hadapsar, Pune, 411 028, India

    Varsha Shriram

  3. Department of Genetics, Osmania University, Hyderabad, 500 007, India

    P. B. Kavi Kishor

  4. Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India

    Narendra Jawali

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  1. Vinay Kumar
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  2. Varsha Shriram
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  5. M. G. Shitole
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Correspondence to M. G. Shitole.

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Kumar, V., Shriram, V., Kavi Kishor, P.B. et al. Enhanced proline accumulation and salt stress tolerance of transgenic indica rice by over-expressing P5CSF129A gene. Plant Biotechnol Rep 4, 37–48 (2010). https://doi.org/10.1007/s11816-009-0118-3

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