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Three dimensional plotted extracellular matrix scaffolds using a rapid prototyping for tissue engineering application

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Naturally derived biomaterials are rarely used in advanced rapid prototyping technology despite their superior biocompatibility. The main problem of natural material plotting is the high sensitivity of materials concentration and viscosity on the plotting nozzle. The aim of the current study was to develop a three dimensional (3D) plotting system capable of dispensing extracellular matrix (ECM)-c (ECM powder blended collagen) and manufacture various shapes of ECM-c scaffolds to apply for irregular defects. We had adapted a powder-based plotting approach to print the stable 3D construct using only cartilage derived ECM materials. This study successfully developed the plotting method for high viscous ECM-c material and showed the 3D plotted scaffolds with high interconnected pores as well as complex shape. Furthermore, cell culture results proved that plotted ECM-c scaffolds were able to provide a suitable environment for cell attachment, proliferation and chondrogenesis. This study shows the 3D printing feasibility of ECM natural material has demonstrated as a first time. We believe our results will offer a meaningful step toward the 3D scaffold printing based on natural ECM materials for future organ printing.

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Authors and Affiliations

  1. Cell Therapy Center, Ajou University Medical Center, Suwon, Korea

    Bo Ram Song, Soon Sim Yang, He Jin & Byoung-Hyun Min

  2. Department of Molecular Science and Technology, Ajou University, Suwon, Korea

    Bo Ram Song, Soon Sim Yang & Byoung-Hyun Min

  3. Department of Orthopedic Surgery, School of Medicine, Ajou University, Suwon, Korea

    He Jin, Do Young Park & Byoung-Hyun Min

  4. Department of Nature-Inspired Nano Convergence System, Korea Institute of Machinery & Materials, Daejeon, Korea

    Su Hee Lee & Jun Hee Lee

  5. Department of Physiology, College of Medicine, Inha University, Incheon, Korea

    So Ra Park

  6. Department of Biomedical Engineering, Jungwon University, Goesan, Korea

    Sang-Hyug Park

  7. Department of Biomedical Engineering, Jungwon University, 85 Munmu-ro, Goesan, 367-805, Korea

    Sang-Hyug Park

  8. Department of Orthopedic Surgery, School of Medicine, Ajou University, 164 World cup-ro, Yeongtong-gu, Suwon, 443-380, Korea

    Byoung-Hyun Min

Authors
  1. Bo Ram Song
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  2. Soon Sim Yang
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  3. He Jin
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  8. Sang-Hyug Park
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  9. Byoung-Hyun Min
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Corresponding authors

Correspondence to Sang-Hyug Park or Byoung-Hyun Min.

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Song, B.R., Yang, S.S., Jin, H. et al. Three dimensional plotted extracellular matrix scaffolds using a rapid prototyping for tissue engineering application. Tissue Eng Regen Med 12, 172–180 (2015). https://doi.org/10.1007/s13770-015-0107-2

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  • DOI: https://doi.org/10.1007/s13770-015-0107-2

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