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Three-dimensional printing of antibiotics-loaded poly-ε-caprolactone/poly(lactic-co-glycolic acid) scaffolds for treatment of chronic osteomyelitis

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Osteomyelitis, an infection and inflammation of bone marrow, often progresses to chronic stage because of delay in diagnosis and treatment. Once it becomes chronic, intravenous antibiotics therapy is no longer effective as swollen surrounding tissue interrupts blood flow into the infected tissue. In severe cases, debridement of the necrotic tissue becomes necessary to prevent further infection. In this study, for the first time, we produced three-dimensional (3D) printed antibiotics-loaded biodegradable poly-e-caprolactone/poly(lactic-co-glycolic acid) scaffold for treatment of chronic osteomyelitis. Subsequent bone regeneration in debrided site was also observed with the customized scaffolds fabricated using 3D printing. Tobramycin, one of the most widely used antibiotics in orthopedic surgery, was chosen due to its thermostable nature compliant to the heat-based fabrication conditions. In in vitro tests, antibacterial and anti-inflammatory effects and release profile of tobramycin from the scaffold were evaluated to verify the potential of our scaffold as a drug delivery system. In addition, in vivo efficacy of the developed drug loaded scaffolds for treatment of chronic osteomyelitis was also examined in a rat model.

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Authors and Affiliations

  1. Department of Mechanical Engineering, Korea Polytechnic University, Siheung, Korea

    Jin-Hyung Shim

  2. Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang, Korea

    Min-Joo Kim & Ju Young Park

  3. Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea

    Ruby Gupta Pati & Dong-Woo Cho

  4. Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, Seoul, Korea

    Young-Pil Yun, Sung Eun Kim & Hae-Ryong Song

Authors
  1. Jin-Hyung Shim
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  2. Min-Joo Kim
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  3. Ju Young Park
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Correspondence to Hae-Ryong Song or Dong-Woo Cho.

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Shim, JH., Kim, MJ., Park, J.Y. et al. Three-dimensional printing of antibiotics-loaded poly-ε-caprolactone/poly(lactic-co-glycolic acid) scaffolds for treatment of chronic osteomyelitis. Tissue Eng Regen Med 12, 283–293 (2015). https://doi.org/10.1007/s13770-015-0014-6

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  • DOI: https://doi.org/10.1007/s13770-015-0014-6

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