Abstract
In this study, silver nanoparticles (Ag NPs) were successfully synthesized by using the green chemistry method, which is simple, environment friendly, and economical by using plant extract obtained from Abelmoschus esculentus. The synthesized Ag NPs were characterized using ultraviolet–visible (UV–Vis) spectroscopy, thermogravimetric analysis, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray crystallography and X-ray photoelectron spectroscopy. The synthesized Ag NPs showed absorbance peaks at 432 nm in the UV–Vis spectroscopy. The mean particle size of Ag NPs was found to be 8.96 nm. The antimicrobial activity of Ag NPs was examined on Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis) and Methicillin-resistant Staphylococcus aureus. Besides, the antibacterial activity of Ag NPs was compared with commercially available antibiotics (Penicillin–streptomycin and Ampicillin–sulbactam). Ag NPs exhibited excellent antimicrobial activity against gram-positive and gram-negative bacteria. In addition, Ag NPs were used in ceramic glaze, and thus, an antibacterial ceramic glaze was developed.
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References
Aboutorabi SN, Nasiriboroumand M, Mohammadi P, Sheibani H, Barani H (2019) Preparation of antibacterial cotton wound dressing by green synthesis silver nanoparticles using mullein leaves extract. J Renew Mater 7:787–794. https://doi.org/10.32604/jrm.2019.06438
Abraham J, Saraf S, Mustafa V, Chaudhary Y, Sivanangam S (2017) Synthesis and evaluation of silver nanoparticles using Cymodocea rotundata against clinical pathogens and human osteosarcoma cell line. J Appl Pharm Sci 7:055–061. https://doi.org/10.7324/japs.2017.70608
AbuDalo MA, Al-Mheidat IR, Al-Shurafat AW, Grinham C, Oyanedel-Craver V (2019) Synthesis of silver nanoparticles using a modified Tollens’ method in conjunction with phytochemicals and assessment of their antimicrobial activity. PeerJ. https://doi.org/10.7717/peerj.6413
Arul D, Balasubramani G, Balasubramanian V, Natarajan T, Perumal P (2017) Antibacterial efficacy of silver nanoparticles and ethyl acetate’s metabolites of the potent halophilic (marine) bacterium, Bacillus cereus A30 on multidrug resistant bacteria. Pathog Glob Health 111:367–382. https://doi.org/10.1080/20477724.2017.1390829
Ataee-Esfahani H, Nemoto Y, Wang L, Yamauchi Y (2011) Rational synthesis of Pt spheres with hollow interior and nanosponge shell using silica particles as template. Chem Commun 47:3885–3887. https://doi.org/10.1039/c0cc05233g
Aygun A, Gülbagca F, Ozer LY et al (2019) Biogenic platinum nanoparticles using black cumin seed and their potential usage as antimicrobial and anticancer agent. J Pharm Biomed Anal 179:112961–112969. https://doi.org/10.1016/j.jpba.2019.112961
Aygün A, Özdemir S, Gülcan M, Cellat K, Şen F (2019) Synthesis and characterization of Reishi mushroom-mediated green synthesis of silver nanoparticles for the biochemical applications. J Pharm Biomed Anal 178:112970–112977. https://doi.org/10.1016/j.jpba.2019.112970
Balashanmugam P, Balakumaran MD, Murugan R, Dhanapal K, Kalaichelvan PT (2016) Phytogenic synthesis of silver nanoparticles, optimization and evaluation of in vitro antifungal activity against human and plant pathogens. Microbiol Res 192:52–64. https://doi.org/10.1016/j.micres.2016.06.004
Balouiri M, Sadiki M, Ibnsouda SK (2016) Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal 6:71–79. https://doi.org/10.1016/j.jpha.2015.11.005
Barkalina N, Charalambous C, Jones C, Coward K (2014) Nanotechnology in reproductive medicine: emerging applications of nanomaterials. Nanomed Nanotechnol Biol Med 10:e921–e938. https://doi.org/10.1016/j.nano.2014.01.001
Chairam S, Somsook E (2016) Facile, versatile and green synthesis of silver nanoparticles by mung bean starch and their catalytic activity in the reduction of 4-nitrophenol. Chiang Mai J Sci 43:609–619
Cho K, Wang X, Nie S, Chen Z, Shin DM (2008) Therapeutic nanoparticles for drug delivery in cancer. Clin Cancer Res 14:1310–1316. https://doi.org/10.1158/1078-0432.ccr-07-1441
Dhand C, Dwivedi N, Loh XJ et al (2015) Methods and strategies for the synthesis of diverse nanoparticles and their applications: a comprehensive overview. RSC Adv 5:105003–105037
Duan H, Wang D, Li Y (2015) Green chemistry for nanoparticle synthesis. Chem Soc Rev 44:5778–5792. https://doi.org/10.1039/c4cs00363b
Dubey SP, Lahtinen M, Sillanpää M (2010a) Green synthesis and characterizations of silver and gold nanoparticles using leaf extract of Rosa rugosa. Colloids Surfaces A Physicochem Eng Asp 364:34–41. https://doi.org/10.1016/j.colsurfa.2010.04.023
Dubey SP, Lahtinen M, Sillanpää M (2010b) Tansy fruit mediated greener synthesis of silver and gold nanoparticles. Process Biochem 45:1065–1071. https://doi.org/10.1016/j.procbio.2010.03.024
El-Seedi HR, El-Shabasy RM, Khalifa SAM et al (2019) Metal nanoparticles fabricated by green chemistry using natural extracts: biosynthesis, mechanisms, and applications. RSC Adv 9:24539–24559
Farokhzad OC, Langer R (2009) Impact of nanotechnology on drug delivery. ACS Nano 3:16–20. https://doi.org/10.1021/nn900002m
Farzana R, Iqra P, Shafaq F et al (2017) Antimicrobial behavior of zinc oxide nanoparticles and β-lactam antibiotics against pathogenic bacteria. Arch Clin Microbiol 08:57. https://doi.org/10.4172/1989-8436.100057
Fatimah I, Indriani N (2018) Silver nanoparticles synthesized using Lantana Camara flower extract by Reflux, microwave and ultrasound methods. Chem J Mold 13:95–102. https://doi.org/10.19261/cjm.2017.461
Gavade SJM, Nikam GH, Dhabbe RS, Sabale SR, Tamhankar BV, Mulik GN (2015) Green synthesis of silver nanoparticles by using carambola fruit extract and their antibacterial activity. Adv Nat Sci Nanosci Nanotechnol 6:045015–045021. https://doi.org/10.1088/2043-6262/6/4/045015
Hamouda RA, Hussein MH, Abo-elmagd RA, Bawazir SS (2019) Synthesis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica. Sci Rep 9:13071–13088. https://doi.org/10.1038/s41598-019-49444-y
Jayaprakash N, Vijaya JJ, Kaviyarasu K et al (2017) Green synthesis of Ag nanoparticles using Tamarind fruit extract for the antibacterial studies. J Photochem Photobiol B Biol 169:178–185. https://doi.org/10.1016/j.jphotobiol.2017.03.013
Jayaprakash N, Suresh R, Rajalakshmi S et al (2019) An assortment of synthesis methods of silver nanoparticles: a review. Asian J Chem 31:1405–1412. https://doi.org/10.14233/ajchem.2019.21972
Kahsay MH, RamaDevi D, Kumar YP, Mohan BS, Tadesse A, Battu G, Basavaiah K (2018) Synthesis of silver nanoparticles using aqueous extract of Dolichos lablab for reduction of 4-Nitrophenol, antimicrobial and anticancer activities. OpenNano 3:28–37. https://doi.org/10.1016/j.onano.2018.04.001
Khan I, Saeed K, Khan I (2017) Nanoparticles: properties, applications and toxicities. Arab J Chem. https://doi.org/10.1016/j.arabjc.2017.05.011
Kolya H, Maiti P, Pandey A, Tripathy T (2015) Green synthesis of silver nanoparticles with antimicrobial and azo dye (Congo red) degradation properties using Amaranthus gangeticus Linn leaf extract. J Anal Sci Technol 6:1–7. https://doi.org/10.1186/s40543-015-0074-1
Koskun Y, Şavk A, Şen B, Şen F (2018) Highly sensitive glucose sensor based on monodisperse palladium nickel/activated carbon nanocomposites. Anal Chim Acta 1010:37–43. https://doi.org/10.1016/j.aca.2018.01.035
Küünal S, Rauwel P, Rauwel E (2018) Plant extract mediated synthesis of nanoparticles. In: Emerging applications of nanoparticles and architectural nanostructures: current prospects and future trends. Elsevier, Amsterdam, pp 411–446
Li S, Shen Y, Xie A, Yu X, Qiu L, Zhang L, Zhang Q (2007) Green synthesis of silver nanoparticles using Capsicum annuum L. extract. Green Chem 9:852–858. https://doi.org/10.1039/b615357g
Li C, Iqbal M, Lin J et al (2018a) Electrochemical deposition: an advanced approach for templated synthesis of nanoporous metal architectures. Acc Chem Res 51:1764–1773. https://doi.org/10.1021/acs.accounts.8b00119
Li C, Tan H, Lin J et al (2018b) Emerging Pt-based electrocatalysts with highly open nanoarchitectures for boosting oxygen reduction reaction. Nano Today 21:91–105. https://doi.org/10.1016/j.nantod.2018.06.005
Li C, Iqbal M, Jiang B et al (2019) Pore-tuning to boost the electrocatalytic activity of polymeric micelle-templated mesoporous Pd nanoparticles. Chem Sci 10:4054–4061. https://doi.org/10.1039/c8sc03911a
Loo YY, Rukayadi Y, Nor-Khaizura MAR, Kuan CH, Chieng BW, Nishibuchi M, Radu S (2018) In Vitro antimicrobial activity of green synthesized silver nanoparticles against selected Gram-negative foodborne pathogens. Front Microbiol 9:1555–1562. https://doi.org/10.3389/fmicb.2018.01555
Manoharan M (2008) Research on the frontiers of materials science: the impact of nanotechnology on new material development. Technol Soc 30:401–404. https://doi.org/10.1016/j.techsoc.2008.04.016
Mashrai A, Mahmood Dar A, Asif Sherwani M, Singh BR (2018) Biosynthesis of silver nanoparticles as a platform for biomedicinal application. J Nanosci Nanomed 2:25–33
Miri A, Mousavi SR, Sarani M, Mahmoodi Z (2018) Using Biebersteinia multifida aqueous extract, and the photocatalytic activity of synthesized silver nanoparticles. Orient J Chem 34:1513–1517. https://doi.org/10.13005/ojc/340342
Misra R, Acharya S, Sahoo SK (2010) Cancer nanotechnology: application of nanotechnology in cancer therapy. Drug Discov Today 15:842–850. https://doi.org/10.1016/j.drudis.2010.08.006
Mittal AK, Chisti Y, Banerjee UC (2013) Synthesis of metallic nanoparticles using plant extracts. Biotechnol Adv 31:346–356
Mollick MMR, Rana D, Dash SK et al (2015) Studies on green synthesized silver nanoparticles using Abelmoschus esculentus (L.) pulp extract having anticancer (in vitro) and antimicrobial applications. Arab J Chem 12:2572–2584. https://doi.org/10.1016/j.arabjc.2015.04.033
Mostafa AA, Sayed SRM, Solkamy EN et al (2015) Evaluation of biological activities of chemically synthesized silver nanoparticles. J Nanomater 2015:789178–789185. https://doi.org/10.1155/2015/789178
Nanda A, Saravanan M (2009) Biosynthesis of silver nanoparticles from Staphylococcus aureus and its antimicrobial activity against MRSA and MRSE. Nanomed Nanotechnol Biol Med 5:452–456. https://doi.org/10.1016/j.nano.2009.01.012
Navya PN, Daima HK (2016) Rational engineering of physicochemical properties of nanomaterials for biomedical applications with nanotoxicological perspectives. Nano Converg. https://doi.org/10.1186/s40580-016-0064-z
Ndikau M, Noah NM, Andala DM, Masika E (2017) Green synthesis and characterization of silver nanoparticles using Citrullus lanatus fruit rind extract. Int J Anal Chem. https://doi.org/10.1155/2017/8108504
Ogawa K, Uesugi K, Morishima K (2017) On-chip internalization process of an intracellular nanobot into a single cell. In: Proceedings of the IEEE international conference on micro electro mechanical systems (MEMS)
Olajuyigbe OO, Afolayan AJ (2012) In Vitro antibacterial and time-kill evaluation of the Erythrina caffra thunb. Extract against bacteria associated with diarrhoea. Sci World J 2012:738314–738322. https://doi.org/10.1100/2012/738314
Omara ST, Zawrah MF, Samy AA (2017) Minimum bactericidal concentration of chemically synthesized silver nanoparticles against pathogenic Salmonella and Shigella strains isolated from layer poultry farms. J Appl Pharm Sci 7:214–221. https://doi.org/10.7324/japs.2017.70829
Osuntokun J, Onwudiwe DC (2018) Phyto-mediated synthesis and photocatalytic activity of nanoparticles using aqueous extract of broccoli. Nano Res Appl 4:44. https://doi.org/10.21767/2471-9838-c2-012
Panja S, Chaudhuri I, Khanra K, Bhattacharyya N (2016) Biological application of green silver nanoparticle synthesized from leaf extract of Rauvolfia serpentina Benth. Asian Pac J Trop Dis 6:549–556. https://doi.org/10.1016/s2222-1808(16)61085-x
Qais FA, Shafiq A, Khan HM et al (2019) Antibacterial effect of silver nanoparticles synthesized using Murraya koenigii (L.) against multidrug-resistant pathogens. Bioinorg Chem Appl 2019:1–11. https://doi.org/10.1155/2019/4649506
Quites F, Azevedo CKS, Alves EPP et al (2017) Ag nanoparticles-based zinc hydroxide-layered hybrids as novel and efficient catalysts for reduction of 4-nitrophenol to 4-aminophenol. J Braz Chem Soc 28:106–115. https://doi.org/10.5935/0103-5053.20160152
Ravichandran V, Vasanthi S, Shalini S et al (2019) Green synthesis, characterization, antibacterial, antioxidant and photocatalytic activity of Parkia speciosa leaves extract mediated silver nanoparticles. Results Phys 15:102565. https://doi.org/10.1016/j.rinp.2019.102565
Saadeh Y, Vyas D (2015) Nanorobotic applications in medicine: current proposals and designs. Am J Robot Surg 1:4–11. https://doi.org/10.1166/ajrs.2014.1010
Şahin B, Demir E, Aygün A, Gündüz H, Şen F (2017) Investigation of the effect of pomegranate extract and monodisperse silver nanoparticle combination on MCF-7 cell line. J Biotechnol 260:79–83. https://doi.org/10.1016/j.jbiotec.2017.09.012
Sahni G, Panwar A, Kaur B (2015) Controlled green synthesis of silver nanoparticles by Allium cepa and Musa acuminata with strong antimicrobial activity. Int Nano Lett 5:93–100. https://doi.org/10.1007/s40089-015-0142-y
Samari F, Parkhari P, Eftekhar E et al (2019) Antioxidant, cytotoxic and catalytic degradation efficiency of controllable phyto-synthesised silver nanoparticles with high stability using Cordia myxa extract. J Exp Nanosci 14:141–159. https://doi.org/10.1080/17458080.2019.1687883
Sana SS, Dogiparthi LK (2018) Green synthesis of silver nanoparticles using Givotia moluccana leaf extract and evaluation of their antimicrobial activity. Mater Lett 226:47–51. https://doi.org/10.1016/j.matlet.2018.05.009
Sharma R, Dhillon A, Kumar D (2018) Mentha-stabilized silver nanoparticles for high-performance colorimetric detection of Al(III) in aqueous systems. Sci Rep 8:5189–5202. https://doi.org/10.1038/s41598-018-23469-1
Singh J, Dutta T, Kim KH et al (2018) “Green” synthesis of metals and their oxide nanoparticles: applications for environmental remediation. J Nanobiotechnol 16:84
Tatke P, Jaiswal Y (2011) An overview of microwave assisted extraction and its applications in herbal drug research. Res J Med Plant 5:21–31. https://doi.org/10.3923/rjmp.2011.21.31
Vyas G, Bhatt S, Paul P (2019) Synthesis of calixarene-capped silver nanoparticles for colorimetric and amperometric detection of mercury (Hg II, Hg 0). ACS Omega 4:3860–3870. https://doi.org/10.1021/acsomega.8b03299
Xia Q, Zhang Y, Li Z et al (2019) Red blood cell membrane-camouflaged nanoparticles: a novel drug delivery system for antitumor application. Acta Pharm Sin B 9:675–689. https://doi.org/10.1016/j.apsb.2019.01.011
Zafar S, Zafar A (2019) Biosynthesis and characterization of silver nanoparticles using Phoenix dactylifera fruits extract and their in vitro antimicrobial and cytotoxic effects. Open Biotechnol J 13:37–46. https://doi.org/10.2174/1874070701913010037
Zia F, Ghafoor N, Iqbal M, Mehboob S (2016) Green synthesis and characterization of silver nanoparticles using Cydonia oblong seed extract. Appl Nanosci 6:1023–1029. https://doi.org/10.1007/s13204-016-0517-z
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The authors would like to thank Keramika Ceramics Company, Kutahya, Turkey.
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Elmusa, F., Aygun, A., Gulbagca, F. et al. Investigation of the antibacterial properties of silver nanoparticles synthesized using Abelmoschus esculentus extract and their ceramic applications. Int. J. Environ. Sci. Technol. 18, 849–860 (2021). https://doi.org/10.1007/s13762-020-02883-x
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DOI: https://doi.org/10.1007/s13762-020-02883-x