Abstract
Pinellia ternata, which is a plant species used in traditional Chinese herbal medicine, exhibits diverse pharmacological effects. However, its metabolic activities are complex, and the functional characterization of its genes has been limited. In this study, we overexpressed a P. ternata premature aging-related gene, PtZS1, in Arabidopsis thaliana. The resulting transgenic lines were sensitive to flooding stress conditions. Additionally, their antioxidant capacity as well as proline and soluble sugar contents (i.e., macromolecular substances affecting osmotic balance) were analyzed. The antioxidant activity of the transgenic lines was weaker than that of the wild-type plants, suggesting that the sensitive phenotype of the transgenic lines may be related to antioxidants. Analyses of the expression levels of antioxidant pathway genes in a quantitative real-time PCR assay revealed the genes were expressed at lower levels in the PtZS1-overexpressing lines than in the wild-type plants. These results indicated that the flooding-sensitive phenotype associated with PtZS1 is related to the antioxidant pathway. The findings of this study provide a theoretical and practical basis for future research on the antioxidant pathways of P. ternata. This is also the first study to confirm a role for the P. ternata premature aging-related gene family in response to flooding stress conditions.
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Abbreviations
- ROS:
-
Reactive oxygen species
- SOD:
-
Superoxide dismutase
- CAT:
-
Catalase
- APX:
-
Ascorbate peroxidase
- MDHAR:
-
Mono-ascorbic acid reductase
- DHAR:
-
Dehydroascorbate reductase
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Acknowledgements
This project was supported by grants from the National Natural Science Foundation of China (Grant No. 32001982), Shandong Provincial Natural Science Foundation of China (Grant No. ZR2019PC041, ZR2021MC062), National Key Research and Development Program of China (Grant No. 2019YFD1000104, SQ2020YFF0422322).
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All authors contributed to the study conception and design. RL performed the experiments and wrote the manuscript; WG analyzed the experiment data; PL sampled material and analyzed the experiment data; KL designed the research and revised the manuscript.
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Li, R., Guo, W., Li, P. et al. Pinellia ternata PtZS1 contributes to abiotic stress responses via the antioxidant pathway. J. Plant Biochem. Biotechnol. 32, 597–607 (2023). https://doi.org/10.1007/s13562-023-00844-2
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DOI: https://doi.org/10.1007/s13562-023-00844-2