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UHPLC-MS-based metabolomic approach for the quality evaluation of Pinellia ternata tubers grown in shaded environments

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Abstract

Pinellia ternata is a native herb in China, and its tuber is widely-used in traditional Chinese medicines. It has been identified that the shading treatment promotes tuber production during cultivation. However, the tuber quality in shaded environments is unknown, which limits the scientific cultivation of P. ternata. In this study, a metabolomics approach based on UHPLC-MS was applied to assess the metabolic components of P. ternata in response to shading. Diverse metabolites were profiled using the metabolomics approach. Then, datasets of P. ternata cultivated in natural light (control) and shaded environments were subjected to multivariate analyses. Two P. ternata tuber products were well separated by the PCA. In total, four P. ternata alkaloids with contents that were not altered by the shaded environment were detected. Metabolomic analyses further identified several organic acids [mevalonic acid, 12,13-dihydroxy-9Z-octadecenoic acid (12, 13-DiHOME), urocanic acid, and γ-aminobutyric acid] that were largely enriched in the shaded environment, which likely contributed to tuber quality and growth. This study determined that shading probably improves the quality of P. ternata tubers and laid a foundation for exploring the regulatory mechanism of the shade response in P. ternata.

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References

  1. Moon BC, Kim WJ, Ji Y, Lee YM, Kang YM, Choi G (2016) Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae tuber, and common adulterants via universal DNA barcode sequences. Genet Mol Res 15(1):gmr.15017064

    Article  Google Scholar 

  2. Zhang JY, Guo QS, Zheng DS (2013) Genetic diversity analysis of P. ternata based on SRAP and TRAP markers. Biochem Syst Ecol 50:258–265

    Article  CAS  Google Scholar 

  3. Lin S, Nie B, Yao G, Yang H, Ye R, Yuan Z (2019) Pinellia ternata (Thunb.) makino preparation promotes sleep by increasing REM sleep. Nat Prod Res 33(22):3326–3329

    Article  CAS  Google Scholar 

  4. Li JB, Fang LP, Xue JP, Sheng W (2010) Effects of P. ternata extracts on inhibiting of oviposition and ovicidal action against Plutella xylostella. China J Chin Mater Med 35(9):1108–1111

    Google Scholar 

  5. Ge X, Hao W (2009) Phytochemical properties and quality evaluation methods of P.ternata. China Pharm 18:3–5

    CAS  Google Scholar 

  6. Zeng XQ, Peng ZS (2008) Growth and propagation of wild P. ternata in cultivation. China J Chin Mater Med 33(8):878–883

    Google Scholar 

  7. Xu JY, Dai C, Shan JJ, Xie T, Xie HH, Wang MM, Yang G (2018) Determination of the effect of P. ternata (Thunb.) Breit. on nervous system development by proteomics. J Ethnopharmacol 213:221–229

    Article  Google Scholar 

  8. Yahagi T, Atsumi T, Mano S, Kikuchi Y, Hara Y, Furukawa M, Yang ZG, Matsuzaki K (2021) Quality evaluation of Pinellia tuber by LC-TOF/MS targeted to ephedrine. J Nat Med. https://doi.org/10.1007/s11418-021-01485-2

    Article  PubMed  Google Scholar 

  9. Liu YH, Guo JH, Liu WT, Liang ZS (2015) Research progress on alkaloids from P. ternata. J Northwest Sci-Tech Univ Agric For (Nat Sci Ed) 43(9):172–177

    Google Scholar 

  10. Jie EY, Ryu YB, Choi SA, Ahn MS, Liu JR, Min SR, Kim SW (2015) Mass propagation of microtubers from suspension cultures of P. ternata cells and quantitative analysis of succinic acid in Pinellia tubers. Plant Biotechnol Rep 9(5):331–338

    Article  Google Scholar 

  11. Iwasa M, Iwasaki T, Ono T, Miyazawa M (2014) Chemical composition and major odor-active compounds of essential oil from Pinellia tuber (dried rhizome of P. ternata) as crude drug. J Oleo Sc 63(2):127–135

    Article  CAS  Google Scholar 

  12. He P, Li S, Wang SJ, Yang YC, Shi JG (2005) Study on chemical constituents in rhizome of P. ternata. China J Chin Mater Med 30(9):671–674

    CAS  Google Scholar 

  13. Nagai T, Kiyohara H, Munakata K, Shirahata T, Sunazuka T, Harigaya Y, Yamada H (2002) Pinellic acid from the tuber of P. ternata Breitenbach as an effective oral adjuvant for nasal influenza vaccine. Int Immunopharmacol 2(8):1183–1193

    Article  CAS  Google Scholar 

  14. Oshio H, Tsukui M, Matsuoka T (1978) Isolation of l-ephedrine from “pinelliae tuber.” Chem Pharm Bull 26(7):2096–2097

    Article  CAS  Google Scholar 

  15. Lu HD, Xue T, Zhang AM, Sheng W, Zhu YF, Chang L, Song YX, Xue JP (2013) Construction of an SSH Library of P. ternata under heat stress, and expression analysis of four transcripts. Plant Mol Biol Rep 31(1):185–194

    Article  CAS  Google Scholar 

  16. Meng XH, Zhang YJ, Li PI, Yang DF, Zhai YJ (2007) Effect of shading on the photosynthetic pigment and protective enzyme activities in P. ternata leaves. Acta Bot Bor-Occid Sin 27(6):1167–1171

    CAS  Google Scholar 

  17. Meng XH, Zhang YJ, Zhang HQ, Lu YC, Yue J (2007) Effects of shading treatment on biological characteristics of P. ternate (Thunb.) Breit. J Northwest Sci-Tech Univ Agric For (Nat Sci Ed) 35(3):220–222

    Google Scholar 

  18. Wang X, Xue JP, Zhang AM (2008) Effects of shading on fresh weight and endogenous of P. ternata tuber. Acta Agric Nucl Sin 22(4):514–518

    Google Scholar 

  19. He J, Du L, Liu G, Fu J, He X, Yu J, Shang L (2011) Quality assessment of reporting of randomization, allocation concealment, and blinding in traditional Chinese medicine RCTs: a review of 3159 RCTs identified from 260 systematic reviews. Trials 12:122. https://doi.org/10.1186/1745-6215-12-122

    Article  PubMed  PubMed Central  Google Scholar 

  20. Wu B, Liu M (2009) How to improve the quality of a clinical trial on traditional Chinese medicine for stroke. Stroke 40(11):e641-642

    Article  Google Scholar 

  21. Su WW, Feng YF, Wu Z, Zhang R, Ye WJ, Peng DG (1998) Study on quality evaluation of Chinese traditional patent medicine with chemical patterm recognition (I). China J Chin Mater Med 21(6):311–314

    CAS  Google Scholar 

  22. Wen Y, Zhang M, Liao ZH, Sun M, Chen M (2008) HPLC fingerprint of P. ternata. China J Chin Mater Med 31(4):503–506

    CAS  Google Scholar 

  23. Wu H, Li W, Zhang KW, He LY, Cui XB (2003) Distinctive compound in Rhizome of P. ternata. China J Chin Mater Med 28(9):836–839

    CAS  Google Scholar 

  24. Lee JW, Ji SH, Choi BR, Choi DJ, Lee YG, Kim HG, Kim GS, Kim K, Lee YH, Baek NI, Lee DY (2018) UPLC-QTOF/MS-based metabolomics applied for the quality evaluation of four processed Panax ginseng products. Molecules 23(8):2062

    Article  Google Scholar 

  25. Zhang M, He P, Yu ZL, Du DD (2011) Effects of simulated acid rain and fertilization and shading treatments on the main medicinal ingredient contents of P. ternata. J Southwest Univ (Nat Sci Ed) 33(4):6–11

    Google Scholar 

  26. Zhang YJ, Meng XH, Yang DF, Xu L, Zhang XY (2009) Comparative study on chemical constituents of P. ternata (thumb.) breit. under different light intensities. J Wuhan Bot Res 27(5):533–536

    CAS  Google Scholar 

  27. Zhou J, Wu Y, Long PP, Ho CT, Wang YJ, Kan ZP, Cao LT, Zhang L, Wan XC (2019) LC-MS-Based Metabolomics reveals the chemical changes of polyphenols during high-temperature roasting of large-leaf yellow tea. J Agr Food Chem 67(19):5405–5412

    Article  CAS  Google Scholar 

  28. Gu EJ, Kim DW, Jang GJ, Song SH, Lee JI, Lee SB, Kim BM, Cho YR, Lee HJ, Kim HJ (2017) Mass-based metabolomic analysis of soybean sprouts during germination. Food Chem 217:311–319

    Article  CAS  Google Scholar 

  29. Marti G, Schnee S, Andrey Y, Simoes-Pires C, Carrupt PA, Wolfender JL, Gindro K (2014) Study of leaf metabolome modifications induced by UV-C radiations in representative vitis, cissus and cannabis species by LC-MS based metabolomics and antioxidant assays. Molecules 19(9):14004–14021

    Article  Google Scholar 

  30. Wang JH, Peake DA, Mistrik R, Huang YY (2013) A platform to identify endogenous metabolites using a novel high performance orbitrap MS and the mzClound library. Blood 4:2–8

    Google Scholar 

  31. Wei SH (2008) Effect of different cultivating ways on content of total alkaloid in P. ternata. J Jilin Agric Univer 30(5):708–711

    CAS  Google Scholar 

  32. Yang BY, Li M, Jing Y, Lai YY, Liu JL, Peng L (2018) Establishment of quality evaluation model of material foundation-efficacy-bioactivity of Pinelliae Rhizoma. Chin Tradit Herbal Drugs 49(19):4575–4585

    Google Scholar 

  33. Chen QW, Yan JP, Meng XX, Xu F, Zhang WW, Liao YL, Qu JW (2017) Molecular cloning, characterization, and functional analysis of acetyl-CoA C-Acetyltransferase and mevalonate kinase genes involved in terpene trilactone biosynthesis from Ginkgo biloba. Molecules 22(1):74

    Article  Google Scholar 

  34. Xu Z, Liu Y, Wei P, Feng K, Niu J, Shen G, Lu W, Xiao W, Wang J, Smagghe GJ, Xu Q, He L (2017) High gama-aminobutyric acid contents involved in abamectin resistance and predation, an interesting phenomenon in Spider Mites. Front Physiol 8:216

    PubMed  PubMed Central  Google Scholar 

  35. Chen S, Tan B, Xia Y, Liao S, Wang M, Yin J, Wang J, Xiao H, Qi M, Bin P, Liu G, Ren W, Yin Y (2019) Effects of dietary gamma-aminobutyric acid supplementation on the intestinal functions in weaning piglets. Food Funct 10(1):366–378

    Article  CAS  Google Scholar 

  36. Kobayashi K, Suzuki M, Muranaka T, Nagata N (2018) The mevalonate pathway but not the methylerythritol phosphate pathway is critical for elaioplast and pollen coat development in Arabidopsis. Plant Biotechnol-Nar 35(4):381–385

    Article  CAS  Google Scholar 

  37. Huang TF, Tohge T, Lytovchenko A, Fernie AR, Jander G (2010) Pleiotropic physiological consequences of feedback-insensitive phenylalanine biosynthesis in Arabidopsis thaliana. Plant J 63(5):823–835

    Article  CAS  Google Scholar 

  38. Xue T, Zhang H, Zhang YY, Wei SQ, Chao QJ, Zhu YF, Teng JT, Zhang AM, Sheng W, Duan YB, Xue JP (2019) Full-length transcriptome analysis of shade-induced promotion of tuber production in P. ternata. BMC Plant Bio 19:565

    Article  CAS  Google Scholar 

  39. Taskiran B, Kaya Z (2016) Association genetics of phenylalanine ammonia lyase (PAL) and cinnamyl alcohol dehydrogenase (CAD) enzymes involved in lignin biosynthesis of european black poplar (Populus nigra). Febs J 283:328–328

    Google Scholar 

  40. Pawlak-Sprada S, Arasimowicz-Jelonek M, Podgorska M, Deckert J (2011) Activation of phenylpropanoid pathway in legume plants exposed to heavy metals. Part I. Effects of cadmium and lead on phenylalanine ammonia-lyase gene expression, enzyme activity and lignin content. Acta Biochim Pol. 58(2):211–216

    CAS  PubMed  Google Scholar 

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Funding

This work was supported by the National Natural Science Foundation of China (81803665, 81573518, 31501368), the Project of Natural Science Research of Universities in Anhui Province, China (KJ2018A0403, KJ2019B07, KJ2017A846).

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  1. Tao Xue and Yujie Xiong have contributed equally to this work.

Authors and Affiliations

  1. Anhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, College of Life Sciences, Huaibei Normal University, Huaibei, 235000, China

    Tao Xue, Yujie Xiong, Jiang Shi, Qiujie Chao, Yanfang Zhu, Yongbo Duan, Wei Sheng, Jingtong Teng & Jianping Xue

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  1. Tao Xue
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  2. Yujie Xiong
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  3. Jiang Shi
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  7. Wei Sheng
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Contributions

Tao Xue, Jingtong Teng, and Jianping Xue conceived and designed the experiments. Jingtong Teng and Jianping Xue collected the materials. Tao Xue, Yujie Xiong, Jiang Shi, Qiujie Chao, Yanfang Zhu, Yongbo Duan, and Wei Sheng performed the experiments. Tao Xue, Yujie Xiong, Jingtong Teng, and Jianping Xue interpreted the data and wrote the manuscript. All authors reviewed and approved the manuscript.

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Correspondence to Tao Xue, Jingtong Teng or Jianping Xue.

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Xue, T., Xiong, Y., Shi, J. et al. UHPLC-MS-based metabolomic approach for the quality evaluation of Pinellia ternata tubers grown in shaded environments. J Nat Med 75, 1050–1057 (2021). https://doi.org/10.1007/s11418-021-01550-w

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