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Allantoin mediated regulation of miRNAs for short term salinity stress tolerance in Oryza sativa L. cv. IR-29

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Abstract

Salinity affects plant in a variety of ways, including ion toxicity, osmotic stress and oxidative damage. Allantoin is a nitrogenous compound derived from purine catabolism that contributes to nitrogen recycling in plants. In rice, accumulation of allantoin in response to salinity stress has been reported. But the role of allantoin under salinity stress is not elucidated till now. Recently, miRNAs have emerged as major regulatory molecules which have been shown to regulate gene expressions during salinity stress condition. To understand the effect of exogenous application of allantoin on miRNAs mediated short-term tolerance of salt sensitive rice genotype IR-29, the relative expression of 10 salinity responsive miRNAs were investigated under salinity stress. The result demonstrates that several miRNA changed their expression pattern in presence of allantoin under salinity. For an example, miRNAs such as osa-miRNA393a, osa-miRNA414, osa-miRNA530 and osa-miR818a were down-regulated under salinity stress condition whereas all these four miRNAs were up-regulated under salinity along with allantoin treated condition indicating that this differential expression under allantoin may play an important role for salinity tolerance of IR-29.

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Abbreviations

ABA:

Abscisic acid

cDNA:

Complementary DNA

miRNA:

MicroRNA

mM:

Millimolar

nt:

Nucleotides

ROS:

Reactive oxygen species

SnRNA:

Small nuclear RNA

qRT-PCR:

Real-time quantitative transcription PCR

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Acknowledgements

The authors are grateful to Director, National Institute for Plant Biotechnology (NIPB), New Delhi for providing all the facility. JN is thankfully acknowledges the Council of Scientific & Industrial Research (CSIR), New Delhi for providing the fellowship.

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Authors and Affiliations

  1. ICAR-National Institute for Plant Biotechnology, IARI Campus, Pusa, New Delhi, 110012, India

    Jyoti Nishad, Alok Kumar Panda, Soni Chowrasia & Tapan Kumar Mondal

  2. Department of Botany, Panjab University, Chandigarh, 160014, India

    Jyoti Nishad & Chongtham Nirmala

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  1. Jyoti Nishad
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  2. Alok Kumar Panda
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  3. Soni Chowrasia
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  4. Chongtham Nirmala
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Contributions

TKM conceived the idea and monitored the progress. CN guided JN to do the wet lab work. AKP helped to design the primers. SC and JN developed the manuscript. All contributed to improve the manuscript.

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Correspondence to Tapan Kumar Mondal.

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Nishad, J., Panda, A.K., Chowrasia, S. et al. Allantoin mediated regulation of miRNAs for short term salinity stress tolerance in Oryza sativa L. cv. IR-29. J. Plant Biochem. Biotechnol. 31, 953–960 (2022). https://doi.org/10.1007/s13562-022-00774-5

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