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Potato peels as feedstock for laccase-catalysed synthesis of phellinsin A

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Abstract

Valorisation of food and agricultural waste can provide a cost-effective substrate source for laccase catalysis. This study evaluated the suitability of potato peels as feedstock for laccase-catalysed modification into value-added products. The optimal conditions for extracting the highest yield of chlorogenic acid (CLA) from potato peels were 300 W (ultrasonication power) and 39.375 min (extraction time). Alkaline hydrolysis of CLA extracted from potato peels produced caffeic acid (CFA) (yield 76.1%) which was in turn oxidised by laccase to produce phellinsin A (yield 32.8%, ~ 1/3 of CLA). Transformation from CLA to phellinsin A resulted in ~ two-fold increase in antioxidant capacity. Phellinsin A exhibited 16.7 and 8.6% cytotoxicity activity against MCF-7 (breast cancer) and HEK-293 (human embryonic kidney) cell lines, respectively. The low cytotoxicity of phellinsin A against normal human cells, coupled with its enhanced antioxidant properties, indicates a good antioxidant ingredient. Based on global potato production statistics, this hydrolytic process can annually recover 1.08e4 tons of CFA from potato peels, subsequently producing 3.53e3 tons of phellinsin A by laccase catalysis. Therefore, potato peel waste is a viable substrate source for laccase-catalysed synthesis of value-added bioactive compounds.

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Funding

This study received funding from the National Research Foundation, South Africa (NRF, Grant No. 105889 and 112099).

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  1. Department of Biotechnology and Food Science, Durban University of Technology, P.O. BOX 1334, Durban, 4000, South Africa

    Blessing Nemadziva, Sandile Ngubane, Faith Matiza Ruzengwe, Kabange Kasumbwe & Tukayi Kudanga

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Nemadziva, B., Ngubane, S., Matiza Ruzengwe, F. et al. Potato peels as feedstock for laccase-catalysed synthesis of phellinsin A. Biomass Conv. Bioref. 13, 13871–13882 (2023). https://doi.org/10.1007/s13399-021-02251-w

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