Abstract
Polymers of hydroxy alkanoates (PHA), also known as biodegradable, biocompatible plastic, are potential alternatives to petrochemical-based plastics. PHA is synthesized by microbes in their cytoplasm in the form of inclusion bodies in stress conditions such as nitrogen, oxygen, and phosphorus with excessive amounts of carbon. Sugar extracted from potato peel in the form of hydrolysate was employed as a carbon source for PHA production after acidic hydrolysis. The acid hydrolysis conditions are optimized for dilute acid concentrations and temperatures. The highest sugar-yielding condition (2% 15 min at 121 ℃) was used for submerged fermentation for PHA production by Bacillus circulans MTCC 8167. Fourier transform infrared spectroscopy, nuclear magnetic resonance, and differential scanning calorimetry were used for polymer characterization. Gas chromatography coupled with mass spectrometry confirmed the monomers such as hexadecenoic acid 3-hydroxy, methyl esters, pentadecanoic acid 14 methyl esters, and tetradecanoic acid 12- methyl esters. Crotonic acid assay was used for quantification of PHA and it was found highest (0.232 ± 0.04 g/L) at 37 °C and 36 h of incubation. Hence, potato peel waste could be a potential feedstock for waste to valuable production.
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References
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Acknowledgements
The authors would like to thanks to the funding agencies Department of Biotechnology (DBT) grant no. BT/RLF/Re-entry/40/2017 and Science and Engineering Research Board (SERB) file no. EEQ/2020/000614, Govt. of India for providing the research grant to carry out this work. Also, special thanks to Council of Scientific & Industrial Research (CSIR), Govt. of India, for fellowship of Ms. Sonika. Authors would also like to thanks Department of Chemistry, Delhi Technological University (DTU) for the support and providing testing facilities.
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Present work is funded by DBT (SAN No. 102/IFD/SAN/1276/2019-20) and SERB (File No. EEQ/2020/000614), Govt. of India.
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All authors read and approved the final manuscript, also confirm the contribution to the review article as follows: Experiment design and conceptualization of article: Sonika Kag and Rashmi Kataria; laboratory experiments and analysis: Sonika Kag; manuscript writing: Sonika Kag; Critically revised the work: Rashmi Kataria, Pravir Kumar, and Sonika Kag; Editing: Rashmi Kataria; Supervision: Pravir Kumar and Rashmi Kataria.
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Kag, S., Kumar, P. & Kataria, R. Potato Peel Waste as an Economic Feedstock for PHA Production by Bacillus circulans. Appl Biochem Biotechnol (2023). https://doi.org/10.1007/s12010-023-04741-1
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DOI: https://doi.org/10.1007/s12010-023-04741-1