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Molecular cytogenetic use of BAC clones in Neofinetia falcata and Rhynchostylis coelestis

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Abstract

Orchidaceae is a highly evolved and largest angiosperm family, which includes enormous number of species and their hybrids. Recent molecular cytogenetic studies of orchid hybrids have successfully started to reveal their origin and chromosome evolution. Here, we constructed BAC libraries of the two orchid plants, Neofinetia falcata and Rhynchostylis coelestis, as molecular cytogenetic tools, which can be used for chromosome-based comparisons of specific regions between different species and their hybrids chromosomes. A total of 21,000 and 10,600 BAC clones with average insert sizes of 74.6 and 50.8 kb were obtained for the N. falcata and R. coelestis, respectively. Random BAC FISH analyses of the two orchid species revealed distribution of some repetitive sequences in these orchid chromosomes. Thus, these BAC clones are useful resources for understanding the genomic organization of the orchid plants.

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Abbreviations

BAC:

Bacterial artificial chromosome

FISH:

Fluorescence in situ hybridization

FITC:

Fluorescein isothiocyanate

HMW:

High-molecular-weight

PCR:

Polymerase chain reaction

PFGE:

Pulsed field gel electrophoresis

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Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research (C) (No. 25450006 to Y.M.; No. 25450515 to G.S.), and Scientific Research on Innovative Areas (Nos. 23113006, 23113001 to G.S.) from the Japan Society for Promotion of Science (JSPS).

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Author notes

  1. Atsushi Matsuba

    Present address: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0101, Japan

Authors and Affiliations

  1. Laboratory of Plant Molecular Genetics, Division of Natural Science, Osaka Kyoiku University, 4-698-1, Asahigaoka, Kashiwara, Osaka, 582-8582, Japan

    Atsushi Matsuba, Miki Fujii, Shan Shan Lee, Go Suzuki & Yasuhiko Mukai

  2. Faculty of Allied Health Science, Kansai University of Welfare Science, Osaka, 582-0026, Japan

    Maki Yamamoto

Authors
  1. Atsushi Matsuba
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  2. Miki Fujii
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  3. Shan Shan Lee
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  4. Go Suzuki
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  5. Maki Yamamoto
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  6. Yasuhiko Mukai
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Correspondence to Go Suzuki.

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Matsuba, A., Fujii, M., Lee, S.S. et al. Molecular cytogenetic use of BAC clones in Neofinetia falcata and Rhynchostylis coelestis . Nucleus 58, 207–210 (2015). https://doi.org/10.1007/s13237-015-0147-y

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  • DOI: https://doi.org/10.1007/s13237-015-0147-y

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