Abstract
Structural genomic resources of Allium plants have been less developed because Allium species have a relatively large genome size. In this background, there have been few reports of construction of BAC libraries of Allium species. Nevertheless, the BAC clones of Allium cepa (bulb onion) developed as a partial library can be used for not only molecular cloning of novel onion genes but also used as FISH probes in molecular cytogenetic studies. The BAC sequencing and BAC FISH analyses in A. cepa revealed that the highly repetitive elements, such as retrotransposon, are major components of the large chromosomes. With the development of next-generation sequencing technologies, transcriptome level sequencing and assembly has been applied to several Allium species, such as bulb onion, shallot, garlic, bunching onion, and Chinese chive. To activate the accumulating transcriptome information as practical genomic resources of Allium research, a number of attempts have been made for anchoring unigenes onto the genetic map of Allium species. Several databases are available on the Internet that provide information about Allium transcriptome, genetic map, and DNA markers.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Abdelrahman M, El-Sayed M, Sato S, Hirakawa H, Ito S, Tanaka K, Mine Y, Sugiyama N, Suzuki Y, Yamauchi N, Shigyo M (2017) RNA-sequencing-based transcriptome and biochemical analyses of steroidal saponin pathway in a complete set of Allium fistulosum—A. cepa monosomic addition lines. PLoS ONE 12(8):e0181784. https://doi.org/10.1371/journal.pone.0181784
Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796–815
Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218
Baldwin S, Revanna R, Thomson S, Pither-Joyce M, Wright K, Crowhurst R, Fiers M, Chen L, Macknight R, McCallum JA (2012) A toolkit for bulk PCR-based marker design from next-generation sequence data: application for development of a framework linkage map in bulb onion (Allium cepa L.). BMC Genom 13:637
Deschamps S, Llaca V, May GD (2012) Genotyping-by-sequencing in plants. Biology 1:460–483
Do GS, Suzuki G, Mukai Y (2004) Genomic organization of a novel root alliinase gene, ALL1, in onion. Gene 325:17–24
Gokce A, McCallum J, Sato Y, Havey M (2002) Molecular tagging of the Ms locus in onion. J Am Soc Hortic Sci 127:576–582
Han J, Thamilarasan SK, Natarajan S, Park JI, Chung MY, Nou IS (2016) De Novo assembly and transcriptome analysis of bulb onion (Allium cepa L.) during cold acclimation using contrasting genotypes. PLoS ONE 11:e0161987
Jakse J, Meyer JD, Suzuki G, McCallum J, Cheung F, Town CD, Havey MJ (2008) Pilot sequencing of onion genomic DNA reveals fragments of transposable elements, low gene densities, and significant gene enrichment after methyl filtration. Mol Genet Genomics 280:287–292
Kamenetsky R, Faigenboim A, Mayer ES, Michael TB, Gershberg C, Kimhi S, Esquira I, Shalom SR, Eshel D, Rabinowitch HD, Sherman A (2015) Integrated transcriptome catalogue and organ-specific profiling of gene expression in fertile garlic (Allium sativum L.). BMC Genom 2015(16):12
Khar A, Jakse J, Havey M (2008) Segregations for onion-bulb colors reveal that red is controlled by at least three loci. J Am Soc Hortic Sci 133:42–47
Khosa JS, Lee R, Bräuning S, Lord J, Pither-Joyce M, McCallum J, Macknight RC (2016) Doubled haploid ‘CUDH2107’as a reference for bulb onion (Allium cepa L.) research: development of a transcriptome catalogue and identification of transcripts associated with male fertility. PLoS ONE 11:e0166568
Khrustaleva LI, De Melo PE, van Heusden AW, Kik C (2005) The integration of recombination and physical maps in a large-genome monocot using haploid genome analysis in a trihybrid Allium population. Genetics 169:1673–1685
Kim DW, Jung TS, Nam SH, Kwon HR, Kim A, Chae SH, Choi SH, Kim DQ, Kim RN, Park HS (2009) GarlicESTdb: an online database and mining tool for garlic EST sequences. BMC Plant Biol 9:61
Kim UJ, Birren BW, Slepak T, Mancino V, Boysen C, Kang HL, Simon MI, Shizuya H (1996) Construction and characterization of a human bacterial artificial chromosome library. Genomics 34:213–218
King J, Bradeen J, Bark O, McCallum J, Havey M (1998) A low-density genetic map of onion reveals a role for tandem duplication in the evolution of an extremely large diploid genome. Theor Appl Genet 96:52–62
Kuhl J, Cheung F, Yuan Q, Martin W, Zewdie Y, McCallum J, Catanach A, Rutherford P, Sink K, Jenderek M, Prince JP (2004) A unique set of 11,008 onion expressed sequence tags reveals expressed sequence and genomic differences between the monocot orders Asparagales and Poales. Plant Cell 16:114–125
Labani RM, Elkington TT (1987) Nuclear DNA variation in the genus Allium L. (Liliaceae). Heredity 59:119–128
Lee HR, Eom EM, Lim YP, Bang JW, Lee DH (2003) Construction of a garlic BAC library and chromosomal assignment of BAC clones using the FISH technique. Genome 46:514–520
Lysak M, Fransz P, Schubert I (1998) Cytogenetic analysis of Arabidopsis. In: Salinas J, Sanchez-Serrano JJ (eds) Arabidopsis protocols, 2nd edn. Humana Press, Totowa, New Jersey, pp 173–186
Martin W, McCallum J, Shigyo M, Jakse J, Kuhl J, Yamane N, Pither-Joyce M, Gokce A, Sink K, Town CD, Havey MJ (2005) Genetic mapping of expressed sequences in onion and in silico comparisons with rice show scant colinearity. Mol Genet Genom 274:197–204
Masamura N, McCallum J, Khrustaleva L, Kenel F, Pither-Joyce M, Shono J, Suzuki G, Mukai Y, Yamauchi N, Shigyo M (2012) Chromosomal organization and sequence diversity of genes encoding lachrymatory factor synthase in Allium cepa L. G3(2):643–651
Matsuba A, Fujii M, Lee SS, Suzuki G, Yamamoto M, Mukai Y (2015) Molecular cytogenetic use of BAC clones in Neofinetia falcata and Rhynchostylis coelestis. The Nucleus 58:207–210
McCallum J, Baldwin S, Shigyo M, Deng Y, van Heusden S, Pither-Joyce M, Kenel F (2012) AlliumMap-A comparative genomics resource for cultivated Allium vegetables. BMC Genom 13:168
McCallum J, Clarke A, Pither-Joyce M, Shaw M, Butler R, Brash D, Scheffer J, Sims I, van Heusden S, Shigyo M, Havey MJ (2006) Genetic mapping of a major gene affecting onion bulb fructan content. Theor Appl Genet 112:958–967
McCallum J, Pither-Joyce M, Shaw M, Kenel F, Davis S, Butler R, Scheffer J, Jakse J, Havey MJ (2007) Genetic mapping of sulfur assimilation genes reveals a QTL for onion bulb pungency. Theor Appl Genet 114:815–822
Meer Q, Vries J (1990) An interspecific cross between Allium roylei Stearn and Allium cepa L., and its backcross to A. cepa. Euphytica 47:29–31
Neumann P, KoblÃzková A, Navrátilová A, Macas J (2006) Significant expansion of Vicia pannonica genome size mediated by amplification of a single type of giant retroelement. Genetics 173:1047–1056
Ohara T, Song Y, Tsukazaki H, Wako T, Nunome T, Kojima A (2005) Genetic mapping of AFLP markers in Japanese bunching onion (Allium fistulosum). Euphytica 144(3):255–263
Ohara T, Tsukazaki H, YeonSang S, Wako T, Yamashita K, Kojima A (2009) Mapping of quantitative trait loci controlling seedling growth in bunching onion (Allium fistulosum L.). J Jpn Soc Horticultural Sci 78:436–442
Rajkumar H, Ramagoni RK, Anchoju VC, Vankudavath RN, Syed AU (2015) De novo transcriptome analysis of Allium cepa L. (Onion) bulb to identify allergens and epitopes. PLoS ONE 10:e0135387
Ricroch A, Yockteng R, Brown SC, Nadot S (2005) Evolution of genome size across some cultivated Allium species. Genome 48:511–520
SanMiguel P, Bennetzen JL (1998) Evidence that a recent increase in maize genome size was caused by the massive amplification of intergene retrotransposons. Ann Bot 82:37–44
Scholten OE, van Kaauwen MP, Shahin A, Hendrickx PM, Keizer LC, Burger K, van Heusden AW, van der Linden CG, Vosman B (2016) SNP-markers in Allium species to facilitate introgression breeding in onion. BMC Plant Biol 16:187
Shigyo M, Tashiro Y, Isshiki S, Miyazaki S (1996) Establishment of a series of alien monosomic addition lines of Japanese bunching onion (Allium fistulosum L.) with extra chromosomes from shallot (A. cepa L. Aggregatum group). Genes Genet Syst 71:363–371
Shirasu K, Schulman AH, Lahaye T, Schulze-Lefert P (2000) A contiguous 66-kb barley DNA sequence provides evidence for reversible genome expansion. Genome Res 10:908–915
Sun X, Zhou S, Meng F, Liu S (2012) De novo assembly and characterization of the garlic (Allium sativum) bud transcriptome by Illumina sequencing. Plant Cell Rep 31:1823–1828
Sun XD, Yu XH, Zhou SM, Liu SQ (2016) De novo assembly and characterization of the Welsh onion (Allium fistulosum L.) transcriptome using Illumina technology. Mol Genet Genomics 291:647–659
Suzuki G, Do GS, Mukai Y (2002) Efficient storage and screening system for onion BAC clones. Breeding Sci 52:157–159
Suzuki G, Nishiuchi C, Tsuru A, Kako E, Li J, Yamamoto M, Mukai Y (2013) Cellular localization of mitotic RAD21 with repetitive amino acid motifs in Allium cepa. Gene 514:75–81
Suzuki G, Ogaki Y, Hokimoto N, Xiao L, Kikuchi-Taura A, Harada C, Okayama R, Tsuru A, Onishi M, Saito N, Do GS, Lee SH, Ito T, Kanno A, Yamamoto M, Mukai Y (2012) Random BAC FISH of monocot plants reveals differential distribution of repetitive DNA elements in small and large chromosome species. Plant Cell Rep 31:621–628
Suzuki G, Ura A, Saito N, Do G, So BB, Yamamoto M, Mukai Y (2001) BAC FISH analysis in Allium cepa. Genes Genet Syst 76:251–255
Thomas JW, Summers TJ, Lee-Lin SQ, Maduro VV, Idol JR, Mastrian SD, Ryan JF, Jamison DC, Green ED (2000) Comparative genome mapping in the sequence-based era: early experience with human chromosome 7. Genome Res 10:624–633
Tsukazaki H, Yaguchi S, Shusei Sato S, Hirakawa H, Katayose Y, Kanamori H, Wako T (2015) Development of transcriptome shotgun assembly-derived markers in bunching onion (Allium fistulosum). Mol Breed 35:55
Tsukazaki H, Yamashita K, Yaguchi S, Masuzaki S, Fukuoka H, Yonemaru J, Kanamori H, Kono I, Hang T, Shigyo M, Kojima A (2008) Construction of SSR-based chromosome map in bunching onion (Allium fistulosum). Theor Appl Genet 2008(117):1213–1223
Tsukazaki H, Yamashita K, Yaguchi S, Yamashita K, Hagihara T, Shigyo M, Kojima A, Wako T (2010) Direct determination of the chromosomal location of bunching onion and bulb onion markers using bunching onion-shallot monosomic additions and allotriploid-bunching onion single alien deletions. Theor Appl Genet 122:501–510
Tsukazaki H, Nunome T, Fukuoka H, Kanamori H, Kono I, Yamashita KI, Wako T, Kojima A (2007) Isolation of 1,796 SSR clones from SSR-enriched DNA libraries of bunching onion (Allium fistulosum). Euphytica 157:83–94
van Heusden A, van Ooijen J, Vrielink-van Ginkel R, Verbeek W, Wietsma W, Kik C (2000) A genetic map of an interspecic cross in Allium based on amplified fragment length polymorphism (AFLP™) markers. Theor Appl Genet 100:118–126
Zhang C, Zhang H, Zhan Z, Liu B, Chen Z, Liang Y (2016) Transcriptome analysis of sucrose metabolism during bulb swelling and development in onion (Allium cepa L.). Frontiers. Plant Sci 2016:7
Zhang HB, Wu C (2001) BAC as tools for genome sequencing. Plant Physiol Biochem 39:195–209
Zhang P, Li W, Fellers J, Friebe B, Gill BS (2004) BAC-FISH in wheat identifies chromosome landmarks consisting of different types of transposable elements. Chromosoma 112:288–299
Zhou SM, Chen LM, Liu SQ, Wang XF, Sun XD (2015) De novo assembly and annotation of the Chinese chive (Allium tuberosum Rottler ex Spr.) transcriptome using the Illumina platform. PLoS ONE 10(7):e0133312
Zhu S, Tang S, Tan Z, Yu Y, Dai Q, Liu T (2017) Comparative transcriptomics provide insight into the morphogenesis and evolution of fistular leaves in Allium. BMC Genom 18:60
Zonneveld BJ, Leitch IJ, Bennett MD (2005) First nuclear DNA amounts in more than 300 angiosperms. Ann Bot 96:229–244
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Nature Switzerland AG
About this chapter
Cite this chapter
Suzuki, G., Akyol, T.Y., Sato, S. (2018). Structural and Functional Genomic Resources Developed. In: Shigyo, M., Khar, A., Abdelrahman, M. (eds) The Allium Genomes. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-319-95825-5_11
Download citation
DOI: https://doi.org/10.1007/978-3-319-95825-5_11
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-95824-8
Online ISBN: 978-3-319-95825-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)