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Application of organosilicate matrix based on methyltriethoxysilane, PVA and bacteria Paracoccus yeei to create a highly sensitive BOD

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Abstract

We have studied immobilization of Paracoccus yeei VKM B-3302 cells in an organosilica sol–gel matrix consisting of tetraethoxysilane, methyltriethoxysilane and polyvinyl alcohol as a structure-modifying agent. Optical microscopy showed that higher amounts of methyltriethoxysilane make the solid material structure softer. In addition, formation of structures, probably, with bacterial cells inside was spotted. We have analyzed the catalytic power of the immobilized bacteria and discovered that the material’s catalytic potential is the highest at 50% of methyltriethoxysilane. Therefore, this seems to be the best ratio of precursors in a material for bacteria to become effectively encapsulated. Analysis of the material structure by low-temperature nitrogen absorption and scanning electron microscopy revealed that in the given conditions the material got crack-like mesopores and spherical particles of about 25 µm in diameter with immobilized bacterial cells on their surface. The study found that the fabricated organosilica material can effectively protect bacterial cells against UV radiation, pH change, high salinity and high heavy metal ion concentration.

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Acknowledgements

The reported study was funded by a grant of the President of the Russian Federation for the State Support of Young Russian PhD Scientists, Agreement No. MK-1349.2020.3. and   by RFBR project number No. 20-33-70078.

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Authors and Affiliations

  1. Tula State University, Tula, Russian Federation

    Olga Kamanina, Vyacheslav Arlyapov, Pavel Rybochkin, Daria Lavrova & Olga Ponamoreva

  2. AO “SPETSPRIBOR”, Tula, Russian Federation

    Elena Podsevalova

Authors
  1. Olga Kamanina
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  2. Vyacheslav Arlyapov
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  3. Pavel Rybochkin
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  4. Daria Lavrova
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  5. Elena Podsevalova
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  6. Olga Ponamoreva
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Contributions

KO, AV, PO conceptualization; KO, LD, RP, PE conducted the experiments; KO, AV, PO data analysis.

Corresponding author

Correspondence to Olga Kamanina.

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Kamanina, O., Arlyapov, V., Rybochkin, P. et al. Application of organosilicate matrix based on methyltriethoxysilane, PVA and bacteria Paracoccus yeei to create a highly sensitive BOD. 3 Biotech 11, 331 (2021). https://doi.org/10.1007/s13205-021-02863-z

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  • DOI: https://doi.org/10.1007/s13205-021-02863-z

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