Abstract
This work investigated the properties of Paracoccus yeei VKM B-3302 bacteria isolated from activated sludge and immobilized in an N-vinylpyrrolidone-modified poly(vinyl alcohol) matrix. The developed hydrogel formed a network structure to enable the entrapment of microbial cells with their viability and biocatalytic properties preserved, which ensured the technological possibility of replicating expendable biosensor receptor elements. A new ratio of the components for the synthesis selected in this work enabled producing a copolymer of an earlier undescribed chemical structure, which can be efficiently used for immobilization of highly sensitive P. yeei bacteria. A biological oxygen demand (BOD) biosensor with these bacteria and matrix was shown to possess a long-time stability exceeding that described earlier, to have a broad substrate specificity and to exceed approximately tenfold the nearest analogues by its sensitivity and the lower boundary value of 0.05 mg/dm3. The biosensor enabled assays of water samples initially attributed to pure samples (the BOD range, 0.05–5.0 mg/dm3). BOD assays of water samples from various sources showed the use of the receptor element of this composition to enable the data that closely correlated with the standard method (R2 = 0.9990).
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Acknowledgements
The reported study was funded by the RFBR, project number 20–33-70078, and a grant of the President of the Russian Federation for the State Support of Young Russian PhD Scientists, agreement No. MK-1349.2020.3. The authors acknowledge Victor Selivanov for providing linguistic help.
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Arlyapov, V.A., Yudina, N.Y., Asulyan, L.D. et al. Registration of BOD using Paracoccus yeei bacteria isolated from activated sludge. 3 Biotech 10, 207 (2020). https://doi.org/10.1007/s13205-020-02199-0
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DOI: https://doi.org/10.1007/s13205-020-02199-0