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Characterization of novel LMW glutenin subunit genes at the Glu-M3 locus from Aegilops comosa

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Abstract

We report the characterization of seven novel low-molecular-weight glutenin subunit (LMW-GS) genes from Aegilops comosa (2n = 2x = 14, MM). We found that all seven LMW-GS genes possessed the same primary structure shared by other known LMW-GSs. Three genes (comosa-M1, comosa-M2, and comosa-M3) encode LMW-m-type subunits, two (comosa-I1 and comosa-I2) encode LWM-i-type subunits, and two (comosa-L1 and comosa-L2) encode LWM-l-type subunits. The comosa-M1 possessed seven cysteine residues, which resulted from a single-nucleotide polymorphism (SNP) of the G-A transition in the fifth position of the N-terminal sequence. Two l-type subunits, comosa-L1 and comosa-L2, contained nine cysteine residues with an extra cysteine residue located in the signal peptide and repetitive domain. In addition, a long insertion of 13 residues (LGQQPQ8/LVQQPQ8) was present in the end of the C-terminal II. Phylogenetic analysis implied that the comosa-L2 may be the intermediate type during the evolution of LMW-l and LMW-i-type genes. Our results demonstrated that the novel LMW-GSs, such as comosa-M1, comosa-L1, and comosa-L2, may have positive effects on dough properties.

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Acknowledgements

This work was financially supported by grants from the National Natural Science Foundation of China (no. 31571668), the National Key Research and Development Program (no. 2017YFD0100900), and the Education Department of Sichuan Province (no. 18ZA0392).

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, China

    Lin Huang, Yu He, Yarong Jin, Fang Wang, Jingshu He, Lihua Feng, Dengcai Liu & Bihua Wu

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  1. Lin Huang
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  2. Yu He
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  3. Yarong Jin
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  4. Fang Wang
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  5. Jingshu He
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  8. Bihua Wu
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Correspondence to Bihua Wu.

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Huang, L., He, Y., Jin, Y. et al. Characterization of novel LMW glutenin subunit genes at the Glu-M3 locus from Aegilops comosa. 3 Biotech 8, 379 (2018). https://doi.org/10.1007/s13205-018-1407-8

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