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Genetic characterisation of dough rheological properties in a wheat doubled haploid population: additive genetic effects and epistatic interactions

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Abstract

Doubled haploid lines (n=160) from a cross between wheat cultivars ‘Cranbrook’ (high dough extensibility) and ‘Halberd’ (low dough extensibility) were grown at three Australian locations. The parents differ at all high- and low-molecular-weight glutenin loci. Dough rheological parameters were measured using small-scale testing procedures, and quantitative trait locus (QTL) mapping procedures were carried out using an existing well-saturated genetic linkage map for this cross. Genetic parameters were estimated using three software packages: QTLCartographer, Epistat and Genstat. Results indicated that environmental factors are a major determinant of dough extensibility across the three trial sites, whereas genotypic factors are the major determinants of dough strength. Composite interval mapping analysis across the 21 linkage groups revealed that as expected, the main additive QTLs for dough rheological properties are located at the high- and low-molecular-weight glutenin loci. A new QTL on chromosome 5A for M-extensibility (a mixograph-estimated measure of extensibility) was detected. Analysis of epistatic interactions revealed that there were significant conditional epistatic interactions related with the additive effects of glutenin loci on dough rheological properties. Therefore, the additive genetic effects of glutenins on dough rheological properties are conditional upon the genetic background of the wheat line. The molecular basis of the interactions with the glutenin loci may be via proteins that modify or alter the gluten protein matrix or variations in the expression level of the glutenin genes. Reverse-phase high performance liquid chromatography analysis of the molar number of individual glutenin subunits across the population showed that certain conditional epistases resulted in increased expression of the affected glutenin. The epistatic interactions detected in this study provide a possible explanation of the variable genetic effects of some glutenins on quality attributes in different genetic backgrounds and provide essential information for the accurate prediction of glutenin related variance in marker-assisted wheat breeding.

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References

  • Basten CJ, Weir BS, Zeng ZB (1997) QTL Cartographer: a reference manual and tutorial for QTL mapping. Department of Statistics, North Carolina State University, Raleigh, NC

    Google Scholar 

  • Beavis WD (1994) The power and deceit of QTL experiments: lessons from comparative QTL studies. In: Proceedings of the 49th annual corn and sorghum industry research conference, Washington DC, pp 250–253

  • Beavis WD (1998) QTL analysis: power, precision and accuracy. In: Paterson AH (ed) Molecular dissection of complex traits. CRC, Boca Raton, pp 145–162

    Google Scholar 

  • Bekes F, Gras PW, Anderssen RS, Appels R (2001) Quality traits of wheat determined by small-scale dough testing methods. Aust J Agric Res 52:1325–1338

    Article  CAS  Google Scholar 

  • Branlard G, Dardevet M (1985) Diversity of grain protein and bread wheat quality. II. Correlation between high-molecular-weight subunits of glutenin and flour quality characteristics. J Cereal Sci 3:345–354

    CAS  Google Scholar 

  • Branlard G, Dardevet M (1994) A null Gli-D1 allele with a positive effect on bread wheat quality. J Cereal Sci 20:235–244

    Article  Google Scholar 

  • Briggle LW, Curtis BC (1987) Wheat worldwide. Heyne EG (ed) Wheat and wheat improvement. ASA, Madison, pp 1–31

    Google Scholar 

  • Chalmers KJ, Campbell AW, Kretschmer J, Karakousis A, Henschke PH, Pierens S (2001) Construction of three linkage maps in bread wheat, Triticum aestivum. Aust J Agric Res 52(12):1089–1119

    Article  CAS  Google Scholar 

  • Chase K (1997) User’s manual and tutorial guide for Epistat. http://www.larklab.4bit.net

  • Chase K, Adler FR, Lark KG (1997) Epistat: a computer program for identifying and testing interactions between pairs of quantitative trait loci. Theor Appl Genet 94:724–730

    Google Scholar 

  • Cornish GB, Békés F, Allen HM, Martin DJ (2001) Flour proteins linked to quality traits in an Australian doubled haploid wheat population. Aust J Agric Res 52(12):1339–1348

    Article  CAS  Google Scholar 

  • Eagles HA, Bariana HS, Ogbonnaya FC, Rebetzke GJ, Hollamby GJ, Henry RJ (2001) Implementation of markers in Australian wheat breeding. Aust J Agric Res 52(12):1349–1356

    Article  CAS  Google Scholar 

  • Echermann PJ, Verbyla AP, Cullis BR, Thompson R (2001) The analysis of quantitative traits in wheat mapping populations. Aust J Agric Res 52(12):1195–1206

    CAS  Google Scholar 

  • Eliasson AC, Larsson K (1993) Cereals in breadmaking: a molecular colloidal approach. Dekker, New York

    Google Scholar 

  • Finney KF, Yamazaki WT, Youngs VL, Rubenthaler GL (1987) Quality of hard, soft, and duram wheats. In: Wheat and wheat improvement, 2nd edn. Agronomy Monograph no. 13. ASA, Madison, WI, pp 677–748

  • Flavell RB, Goldsbrough AP, Robert LS, Schnick D, Thompson RD (1989) Genetic variation in wheat HMW glutenin subunits and the molecular basis of breadmaking quality. Biotechnology 7:1281–1285

    Google Scholar 

  • Gras PW, Anderssen RS, Keentok M, Bekes F, Appels R (2001) Gluten protein functionality in wheat flour processing: a review. Aust J Agric Res 52(12):1311–1324

    Article  CAS  Google Scholar 

  • Greene FC, Anderson OD, Yip RE, Halford NG, Malpica-Romero JM, Shewry PR (1988) Proceedings of the 7th international wheat genetics symposium. IPSR, Cambridge, pp 735–740

  • Gupta RB, MacRitchie (1991) A rapid one-step one-dimensional SDS procedure for analysis of subunit composition of glutenin in wheat. J Cereal Sci 14:105–109

    CAS  Google Scholar 

  • Gupta RB, MacRitchie (1994) Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1 of common wheats. II. Biochemical basis of the allelic effects on dough properties. J Cereal Sci 19:19–29

    Article  CAS  Google Scholar 

  • Haley CS, Knott SA (1992) A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity 69:315–324

    Google Scholar 

  • Jackson EA, Holt LM, Payne PI (1983) Characterization of high-molecular-weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two dimensional electrophoresis and the chromosomal localization of their controlling genes. Theor Appl Genet 66:29–37

    CAS  Google Scholar 

  • Jansen RC, Stam P (1994) High resolution of quantitative traits into multiple loci via interval mapping. Genetics 136:1447–1455

    CAS  PubMed  Google Scholar 

  • Kammholz SJ, Campbell AW, Sutherland MW, Hollamby GJ, Martin PJ, Eastwood RF (2001) Establishment and characterisation of wheat genetic mapping populations. Aust J Agric Res 52(12):1079–1088

    Article  CAS  Google Scholar 

  • Kearsey MJ, Pooni HS (1996) The genetical analysis of quantitative traits, 1st edn. Chapman & Hall, London

    Google Scholar 

  • Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199

    CAS  PubMed  Google Scholar 

  • Larroque, OR, Bekes, F, Wrigley, CW, WG Rathmell (2000) Analysis of gluten proteins in grain and flour blends by RP-HPLC. Proceedings Gluten 2000, Bristol, UK, April 2000, pp 136–139

  • Ma W (2001) Molecular mapping of wheat quality attributes in a wheat doubled haploid population W21MMT70 × ‘Mendos’. PhD Dissertation, University of Southern Queensland, Queensland, Australia

  • Melchinger AE, Utz HF, Schön CC (1998) Quantitative trait locus (QTL) mapping using different testers and independent population samples in maize reveals low power of QTL detection and large bias in estimates of QTL effects. Genetics 149:383–403

    CAS  PubMed  Google Scholar 

  • Nieto-Taladriz MT, Perretant MR, Rousset M (1994) Effect of gliadins and HMW and LMW subunits of glutenin on dough properties in the F6 recombinant inbred lines from a bread wheat cross. Theor Appl Genet 88:81–88

    Google Scholar 

  • Payne PI, Holt LM, Law CN (1981) Structural and genetical studies on the high molecular weight of wheat gluten. Part I: Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor Appl Genet 60:229–239

    CAS  Google Scholar 

  • Payne PI, Seekings JA, Worland AJ, Jarvis MG, Holt LM (1987) Allelic variation of glutenin subunits and gliadins and its effects on bread-making quality in wheat: analysis of F5 progeny from ‘Chinese Spring’ × ‘Chinese Spring’ (Hope 1A). J Cereal Sci 6:103–118

    Google Scholar 

  • Pogna NE, Tusa P, Boggini G (1996) Genetic and biochemical aspects of dough quality in wheat. Adv Food Sci 18(5–6):145–151

    CAS  Google Scholar 

  • Simmonds DH (1989) Inherent quality factors in wheat. Wheat and wheat quality in Australia: 31–61. Australia Wheat Board

    Google Scholar 

  • Soller M, Brody T, Genizi A (1976) The power of experimental designs for the detection of linkage between marker loci and quantitative loci in crosses between inbred lines. Theor Appl Genet 47:35–39

    Article  Google Scholar 

  • Varghese JP, Struss D, Kazman ME (1996) Rapid screening of selected European winter wheat varieties and segregating populations for the Glu-D1d allele using PCR. Plant Breed 115:451–454

    Google Scholar 

  • Wall JS (1979) The role of wheat proteins in determining baking quality. In: Laidman DL, Wyn Jones RG (eds) Recent advances in the biochemistry of cereals. Academic, New York, pp 275–311

    Google Scholar 

  • Wang SC, Basten CJ, Zeng ZB (2002) Windows QTLCartographer. Statistical genetics, North Carolina State University

  • Zeng ZB(1993) Theoretical basis for separation of multiple linked gene effects in mapping quantitative trait loci. Proc Natl Acad Sci USA 90:10972–10976

    CAS  PubMed  Google Scholar 

  • Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468

    CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. CSIRO Plant Industry, GPO Box 1600, ACT 2601, Australia

    W. Ma, F. Bekes, O. Larroque, M. K. Morell & K. R. Gale

  2. Western Australia Department of Agriculture and CRC for Molecular Plant Breeding, Biological Sciences, Murdoch University, WA, 6152, Australia

    W. Ma & R. Appels

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  1. W. Ma
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  2. R. Appels
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  3. F. Bekes
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  4. O. Larroque
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  5. M. K. Morell
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  6. K. R. Gale
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Correspondence to K. R. Gale.

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Communicated by A. Charcosset

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Ma, W., Appels, R., Bekes, F. et al. Genetic characterisation of dough rheological properties in a wheat doubled haploid population: additive genetic effects and epistatic interactions. Theor Appl Genet 111, 410–422 (2005). https://doi.org/10.1007/s00122-005-2001-0

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  • DOI: https://doi.org/10.1007/s00122-005-2001-0

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