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Expression profile of phenylpropanoid pathway genes in Decalepis hamiltonii tuberous roots during flavour development

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Abstract

To explore the transcriptional control of phenylpropanoid pathway (PPP) involved in vanillin flavour metabolites production in tuberous roots of Decalepis hamiltonii, four PPP key genes expressed during the tuber development were identified and their mRNA expression profiles were evaluated using quantitative real-time PCR. Flavour metabolite quantification by HPLC analysis confirmed 10, 170 and 500 µg/g 2-hydroxy-4-methoxy benzaldehyde and 4, 20 and 40 µg/g vanillin in first- (3-month-old plant), second- (18-month-old plant) and third-stage tubers (60-month-old matured plant), respectively. The expression of all four genes phenylalanine ammonia lyase (DhPAL), cinnamate-4-hydroxylase (DhC4H), caffeic acid-O-methyltransferase (DhCOMT) and vanillin synthase (DhVAN) increased with flavour development from first stage to second stage. A decrease in expression from 1.9-folds to 0.1-folds and 19.2-folds to 5.2-folds for DhCOMT and DhVAN was recorded for second stage to third stage, respectively. However, a gradual increase in expression of DhPAL (up to 26.4-folds) and a constant expression pattern for DhC4H (up to 7.1-folds) was evident from second stage to third stage of flavour development. The decrease in the expression levels of DhCOMT and DhVAN in third stage shows that the second-stage tubers are more transcriptionally active towards flavour biosynthesis.

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Acknowledgements

We thank Dr. Rajashekar B. (FSAQCL, CSIR-CFTRI) for helping us in gene sequencing and Ms. Asha (CIFS, CSIR-CFTRI) for her help in performing HPLC. K.K, P.S and L.V thank CSIR for providing fellowship.

Funding

This work was funded by Science and Engineering Research Board, Department of Science and Technology, New Delhi (Grant no. EMR/2016/001049).

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  1. Plant Cell Biotechnology Department, CSIR-CFTRI, Mysore, 570020, India

    Kamireddy Kiran, Priyanka Purushottam Sonbarse, Lokesh Veeresh, Nandini P. Shetty & Giridhar Parvatam

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  1. Kamireddy Kiran
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  2. Priyanka Purushottam Sonbarse
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Contributions

PG participated in designing of the study drafting of the manuscript and analysis of results. LV participated in designing of degenerate primers, availing accession numbers to gene sequences and helped in performing the experiments and their analysis. PS performed the HPLC and MS analysis, statistical analysis and participated in drafting the manuscript. NPS involved in discussions and helped in improvement of manuscript. KK designed the study, identified the gene sequences, performed qRT-PCR analysis, analysed the results and drafted the manuscript.

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Correspondence to Giridhar Parvatam.

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Kiran, K., Sonbarse, P.P., Veeresh, L. et al. Expression profile of phenylpropanoid pathway genes in Decalepis hamiltonii tuberous roots during flavour development. 3 Biotech 8, 365 (2018). https://doi.org/10.1007/s13205-018-1388-7

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