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Biodegradation of pyrene and benzo[a]pyrene in the liquid matrix and soil by a newly identified Raoultella planticola strain

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Abstract

In the current study, the PL7 strain was isolated from soil and identified as Raoultella planticola based on its physiological characteristics and 16S rDNA sequence. By the 10th day, the PL7 strain degraded 52.0% of the pyrene (PYR) content and 50.8% of the benzo[a]pyrene (BaP) content in 20 mg L−1 PYR and 10 mg L−1 BaP in the liquid matrix. The half-life of PYR and BaP by PL7 degradation was 8.59 and 9.46 days, respectively. At pH 8.0, the degradation rates of PYR and BaP by PL7 were significantly higher at 30 °C than at 20 and 40 °C. The degradation ability of PL7 differed in red soil, paddy soil and fluvo-aquic soil; red soil produced the fastest degradation rates. The half-life of PYR and BaP by PL7 degradation in red soil was 21.7 and 11.9 days, respectively; however, without PL7 the half-life of PYR in red soil was 91.2 days. This study demonstrated the significant potential of the PL7 strain for bioremediation applications in the liquid matrix and soil contaminated by PAHs.

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Acknowledgements

This study was financially supported by the National Natural Science Foundation of China (No. 21007061, No. 21307115), the opening foundation of the Key Laboratory of Recycling and Eco-treatment of Waste Biomass of the Zhejiang Province (No. 2016REWB08), the China Spark Program (2015GA710001), the Scientific Research Foundation of Zhejiang University of Science and Technology (No. F701104F07), the Program for International S&T Cooperation Projects of China (2014DFE90040) and the Key Project in the Youth Elite Support Plan of the Zhejiang Academy of Agricultural Sciences.

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Authors and Affiliations

  1. Key Laboratory of Recycling and Eco-treatment of Waste Biomass of Zhejiang Province, Zhejiang University of Science and Technology, Hangzhou, 310023, China

    Lifeng Ping & Xiaoli Yuan

  2. Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Lifeng Ping, Qian Guo, Xiaoyang Chen, Chunrong Zhang & Hua Zhao

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  1. Lifeng Ping
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  2. Qian Guo
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Correspondence to Lifeng Ping.

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Ping, L., Guo, Q., Chen, X. et al. Biodegradation of pyrene and benzo[a]pyrene in the liquid matrix and soil by a newly identified Raoultella planticola strain. 3 Biotech 7, 56 (2017). https://doi.org/10.1007/s13205-017-0704-y

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