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Anaerobic biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by a facultative anaerobe Pseudomonas sp. JP1

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Abstract

Polycyclic aromatic hydrocarbons (PAHs) are harmful persistent organic pollutants, while the high-molecular-weight (HMW) PAHs are even more detrimental to the environment and human health. However, microbial anaerobic degradation of HMW PAHs has rarely been reported. One facultative anaerobe Pseudomonas sp. JP1 was isolated from Shantou Bay, Shantou, China, which could degrade a variety of HMW PAHs. After 40 days cultivation with strain JP1, anaerobic biodegradation rate of benzo[a]pyrene (BaP), fluoranthene, and phenanthrene was 30, 47, and 5 %, respectively. Consumption of nitrate as the electron acceptor was confirmed by N-(1-naphthyl) ethylenediamine spectrophotometry. Supplementation of sodium sulfite, maltose, or glycine, and in a salinity of 0–20 ‰ significantly stimulated anaerobic degradation of BaP. Lastly, the anaerobic degradation metabolites of BaP by strain JP1 were investigated using GC/MS, and the degradation pathway was proposed. This study is helpful for further studies on the mechanism of anaerobic biodegradation of PAHs.

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References

  • Annweiler E, Michaelis W, Meckenstock RU (2002) Identical ring cleavage products during anaerobic degradation of naphthalene, 2-methylnaphthalene, and tetralin indicate a new metabolic pathway. Appl Environ Microbiol 68:852–858

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Coates JD, Woodward J, Allen J, Philip P, Lovley DR (1997) Anaerobic degradation of polycyclic aromatic hydrocarbons and alkanes in petroleum-contaminated marine harbor sediments. Appl Environ Microbiol 63:3589–3593

    PubMed  CAS  PubMed Central  Google Scholar 

  • Coates JD, Chakraborty R, Lack JG, O’Connor SM, Cole KA, Bender KS, Achenbach LA (2001) Anaerobic benzene oxidation coupled to nitrate reduction in pure culture by two strains of dechloromonas. Nature 411:1039–1043

    Article  PubMed  CAS  Google Scholar 

  • Dandie CE, Thomas SM, Bentham RH, McClure NC (2004) Physiological characterization of Mycobacterium sp. strain 1B isolated from a bacterial culture able to degrade high-molecular-weight polycyclic aromatic hydrocarbons. J Appl Microbiol 97:246–255

    Article  PubMed  CAS  Google Scholar 

  • Fuchs G, Boll M, Heider J (2011) Microbial degradation of aromatic compound—from one strategy to four. Nat Rev Microbiol 10:1–12

    Article  Google Scholar 

  • Haritash AK, Kaushik CP (2009) Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): a review. J Hazard Mater 169:1–15

    Article  PubMed  CAS  Google Scholar 

  • Johnsen AR, Wick LY, Harms H (2005) Principles of microbial PAH degradation in soil. Environ Pollut 133:71–84

    Article  PubMed  CAS  Google Scholar 

  • Li CH, Wong YS, Tam NFY (2010) Anaerobic biodegradation of polycyclic aromatic hydrocarbons with amendment of iron (III) in mangrove sediment slurry. Bioresour Technol 101:8083–8092

    Article  PubMed  CAS  Google Scholar 

  • Lily YY, Phelps CD (2005) Metabolic biomarkers for monitoring in situ anaerobic hydrocarbon degradation. Environ Health Perspect 113:62–67

    Google Scholar 

  • Luan T, Fang S, Zhong Y, Lin L, Chan SMN, Lan C, Tam NFY (2007) Determination of hydroxy metabolites of polycyclic aromatic hydrocarbons by fully automated solid-phase microextraction derivatization and gas chromatography–mass spectrometry. J Chromatogr A 1173:37–43

    Article  PubMed  CAS  Google Scholar 

  • Mahmood Q, Zheng P, Cai J, Wu D, Hu B, Li J (2007) Anoxic sulfide bio-oxidation using nitrite as electron acceptor. J Hazard Mater 147(1):249–256

    Article  PubMed  CAS  Google Scholar 

  • Meckenstock RU, Safinowski M, Griebler C (2004) Anaerobic degradation of polycyclic aromatic hydrocarbons. FEMS Microbiol Ecol 49(1):27–36

    Article  PubMed  CAS  Google Scholar 

  • Nieman JKC, Sims RC, Mclean JE, Sorensen D (2001) Fate of pyrene in contaminated soil amended with alternate electron acceptors. Chemosphere 44:1265–1271

    Article  PubMed  CAS  Google Scholar 

  • Oppenheimer CH (1952) The growth and viability of sixty-three species of marine bacteria as influenced by hydrostatic pressure. J Mar Res 11:10–18

    Google Scholar 

  • Rafin C, Veignie E, Fayeulle A, Surpateanu G (2009) Benzo[a]pyrene degradation u sing simultaneously combined chemical oxidation, biotreatment with Fusarium solani and cyclodextrins. Bioresour Technol 100:3157–3160

    Article  PubMed  CAS  Google Scholar 

  • Rockne KJ, Strand SE (2001) Anaerobic biodegradation of naphthalene, phenanthrene and biphenyl by a denitrifying enrichment culture. Water Res 35:291–299

    Article  PubMed  CAS  Google Scholar 

  • Rothermich MM, Hayes LA, Lovley DR (2002) Anaerobic, sulfate-dependent degradation of polycyclic aromatic hydrocarbons in petroleum-contaminated harbor sediments. Environ Sci Technol 36:4811–4817

    Article  PubMed  CAS  Google Scholar 

  • Schreiber K, Benkert B, Eschbach M, Jahn D (2007) The anaerobic regulatory network required for Pseudomonas aeruginosa nitrate respiration. J Bacteriol. 3:4310–4314

    Article  Google Scholar 

  • Shi DQ, Zhao JS, Yang JR, Hou YF, Kong Y (2004) Degradation of dibenzothiophene by Pseudomonas stutzeri UPl. China Environ Sci 24:730–733

    CAS  Google Scholar 

  • Song L, Wang H, Shi IHC (2007) The function study on trichlorobenzene degradation by plasmid pT7. China Environ Sci 27:121–124

    CAS  Google Scholar 

  • Song XH, Xu Y, Li GM, Zhang Y, Huang TW, Hu Z (2011) Isolation, characterization of Rhodococcus sp. P 14 capable of degrading high-molecular-weight polycyclic aromatic hydrocarbons and aliphatic hydrocarbons. Mar Pollut Bull 62:2122–2128

    Article  PubMed  CAS  Google Scholar 

  • Strohm TO, Walter BJ, Schink B (2007) Growth yields in bacterial denitrification and nitrate ammonification. Appl Environ Microbiol 73:1420–1424

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Sun M, Ye M, Hu F, Li H, Teng Y, Luo Y, Jiang X, Kengara FO (2014) Tenax extraction for exploring rate-limiting factors in methyl-β-cyclodextrin enhanced anaerobic biodegradation of PAHs under denitrifying conditions in a red paddy soil. J Hazard Mater 264:505–513

    Article  PubMed  CAS  Google Scholar 

  • Thauer RK, Jungermann K, Decker K (1977) Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev 41:100–180

    PubMed  CAS  PubMed Central  Google Scholar 

  • Verdin A, Anissa Sahraoui LH, Durand R (2004) Degradation of benzo[a]pyrene by mitosporic fungi and extracellular oxidative enzymes. Int Biodeter Biodegr 53:65–70

    Article  CAS  Google Scholar 

  • Wu YR, He TT, Zhong MQ, Zhang YL, Li EM, Huang TW, Hu Z (2009) Isolation of marine benzo[a]pyrene-degrading Ochrobactrum sp. BAP5 and proteins characterization. J Environ Sci 21:1446–1451

    Article  CAS  Google Scholar 

  • Yan Z, Jiang H, Li X, Shi Y (2014) Accelerated removal of pyrene and benzo[a]pyrene in freshwater sediments with amendment of cyanobacteria-derived organic matter. J Hazard Mater 272:66–74

    Article  PubMed  CAS  Google Scholar 

  • Zhang X, Sullivan ER, Young LY (2000) Evidence for aromatic ring reduction in the biodegradation pathway of carboxylated naphthalene by a sulfate reducing consortium. Biodegradation 11(2–3):17–124

    Google Scholar 

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Acknowledgments

This work was supported partly by National Science Foundation of China (Nos.41076073, 30970106).We thank Mrs. Xiaoshan Zhang, Mr. Tufeng Chen for assistance in GC/MS and HPLC analysis. We are grateful to Prof. Guangming Xiong (Kiel University) for helpful suggestions.

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Authors and Affiliations

  1. College of Science, Shantou University, 243 Daxue Road, Shantou, 515063, Guangdong, China

    Lei Liang, Xiaohui Song, Jing Kong, Chenghui Shen, Tongwang Huang & Zhong Hu

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  1. Lei Liang
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  2. Xiaohui Song
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  3. Jing Kong
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  4. Chenghui Shen
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  5. Tongwang Huang
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  6. Zhong Hu
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Correspondence to Zhong Hu.

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Liang, L., Song, X., Kong, J. et al. Anaerobic biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by a facultative anaerobe Pseudomonas sp. JP1. Biodegradation 25, 825–833 (2014). https://doi.org/10.1007/s10532-014-9702-5

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  • DOI: https://doi.org/10.1007/s10532-014-9702-5

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