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Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system

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Abstract

Mannanases, one of the important enzyme group for industry, are produced by numerous filamentous fungi, especially Aspergillus species with different fermentation methods. The aim of this study was to show the best fermentation method of β-mannanase production for fungal growth in fermenter. Therefore, different fermentation strategies in fed-batch fermentation (suspended, immobilized cell, biofilm and microparticle-enhanced bioreactor) were applied for β-mannanase production from glucose medium (GM) and carob extract medium (CEM) by using recombinant Aspergillus sojae. The highest β-mannanase activities were obtained from microparticle-enhanced bioreactor strategy. It was found to be 347.47 U/mL by adding 10 g/L of Al2O3 to GM and 439.13 U/mL by adding 1 g/L of talcum into CEM. The maximum β-mannanase activities for suspended, immobilization, and biofilm reactor remained at 72.55 U/mL in GM, 148.81 U/mL in CEM, and 194.09 U/mL in GM, respectively. The reason for that is the excessive, and irregular shaped growth and bulk formation, inadequate oxygen transfer or substrate diffusion in bioreactor. Consequently, the enzyme activity was significantly enhanced by addition of microparticles compared to other fed-batch fermentation strategies. Also, repeatable β-mannanase activities were obtained by controlling of the cell morphology by adding microparticle inside the fermenter.

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Acknowledgements

This study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) foundation (Grant No. #112O167).

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Authors and Affiliations

  1. Department of Food Engineering, Akdeniz University, 07058, Antalya, Turkey

    Mustafa Germec, Ercan Yatmaz, Ercan Karahalil & İrfan Turhan

  2. Department of Food Engineering, Cankiri Karatekin University, 18100, Cankiri, Turkey

    Mustafa Germec

  3. Göynük Culinary Arts Vocational School, Akdeniz University, 07980, Antalya, Turkey

    Ercan Yatmaz

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  1. Mustafa Germec
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  2. Ercan Yatmaz
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  3. Ercan Karahalil
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Correspondence to İrfan Turhan.

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All the authors in this study mutually agree for submitting our manuscript to 3 Biotech and declare that they have no conflict of interest in the publication.

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Germec, M., Yatmaz, E., Karahalil, E. et al. Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system. 3 Biotech 7, 77 (2017). https://doi.org/10.1007/s13205-017-0694-9

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