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Voltammetric determination of polyphenolic content as rosmarinic acid equivalent in tea samples using pencil graphite electrodes

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Abstract

The quasi-reversible, diffusion controlled behavior of rosmarinic acid (RA) on a disposable pencil graphite electrode (PGE) was established by cyclic voltammetry. Using the anodic oxidation peak presented by RA on the PGE a differential pulse voltammetric (DPV) method was developed for the quantitative determination of RA. The linear range was 10−8 – 10−5 M RA and the detection and quantification limits were 7.93 × 10−9 M and 2.64 × 10−8 M RA, respectively. The applicability of the developed method was tested by recovery studies and by the assessment of the total polyphenolic contents (TPCDPV) of green, white and black Turkish teas, which were found to be 40.74, 30.04 and 23.97 mg rosmarinic acid equivalent/g dry tea, respectively. These results were in good agreement with those obtained by the Folin-Ciocalteu method. The developed method is a sensitive and cheap tool for the rapid and precise evaluation of TPCDPV of tea samples.

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Acknowledgments

This work was supported by the Romania-Turkey Joint Project financed by the Executive Unit for Financing Higher Education, Research and Development and Innovation (UEFISCDI) (604/2013) and The Scientific and Technological Research Council of Turkey (Tubitak) (112 O 420).

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Av. District 3, 030018, Bucharest, Romania

    Iulia Gabriela David, Mihaela Buleandră, Dana Elena Popa, Ana-Maria Cristina Bîzgan, Zenovia Moldovan, Irinel-Adriana Badea & Emilia Elena Iorgulescu

  2. Molecular Biology Genetics and Bioengineering Program, Sabanci University, Orhanlı-Tuzla, 34956, Istanbul, Turkey

    Tuğçe Ayça Tekiner & Huveyda Basaga

Authors
  1. Iulia Gabriela David
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  2. Mihaela Buleandră
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  3. Dana Elena Popa
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  4. Ana-Maria Cristina Bîzgan
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  5. Zenovia Moldovan
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  6. Irinel-Adriana Badea
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  7. Emilia Elena Iorgulescu
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  8. Tuğçe Ayça Tekiner
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  9. Huveyda Basaga
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Correspondence to Mihaela Buleandră.

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Highlights

• Voltammetric investigation of rosmarinic acid on a cheap, disposable pencil graphite electrode (PGE)

• Rapid, simple and sensitive voltammetric method on PGE for rosmarinic acid quantification

• Cheap tool for the rapid assay of the total polyphenolic content of teas (mg RAE/g tea)

• Precision of the developed voltammetric method comparable to that of Folin-Ciocalteu method

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David, I.G., Buleandră, M., Popa, D.E. et al. Voltammetric determination of polyphenolic content as rosmarinic acid equivalent in tea samples using pencil graphite electrodes. J Food Sci Technol 53, 2589–2596 (2016). https://doi.org/10.1007/s13197-016-2223-y

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  • DOI: https://doi.org/10.1007/s13197-016-2223-y

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