Skip to main content
SpringerLink
Log in

Vitamin B12 biosynthesis over waste frying sunflower oil as a cost effective and renewable substrate

  • Original Article
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

Statistical experimental designs were used to develop a medium based on waste frying sunflower oil (WFO) and other nutrient sources for production of vitamin B12 (VB12) by Propionibacterium freudenreichii subsp. freudenreichii PTCC 1674. The production of acetic acid and propionic acid were also evaluated using the same microorganism. The amount of WFO in the media was initially optimized. The amount of 4 % w/v of oil found to be an appropriate amount for production of VB12. A Plackett Burman design was then employed to identify nutrients that have significant effect on the production of VB12 in the WFO media. Dimethylbenzimidazolyl (DMB), cobalt chloride, ferrous sulfate, and calcium chloride were the most important compounds. The level optimization of nutrients as the significant factors was finally performed using response surface methodology based on a central composite design. The model predicted that a medium containing 35.56 mg/L DMB, 14.69 mg/L CoCl2.6H2O, 5.82 mg/L FeSO4.7H2O, and 11.41 mg/L CaCl2.2H2O gives the maximum VB12 production of 2.60 mg/L. The optimized medium provides a final concentration of vitamin 170 % higher than that by the original medium. This study offers valuable insights on a cost-effective carbon source for industrial production of food-grade VB12.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig 5

Similar content being viewed by others

References

  • Berry EC, Bullerman LB (1996) Use of cheese whey for vitamin B12 production. II.Cobalt precursor and aeration levels. J Appl Microbiol 3:356–357

    Google Scholar 

  • Burgess MC, Smid EJ, Sinderen DV (2009) Bacterial vitamin B2, B11 and B12 overproduction: an overview. Int J Food Microbiol 133:1–7

    Article  CAS  Google Scholar 

  • Chiliveri SR, Yeruva T, Panda SH, Linga VR (2010) Optimization of fermentation parameters for vitamin B12 production using Propionibacterium freudenreichii subsp. shermanii OLP-5 by Taguchi Method. J Pure Appl Microbiol 4(2):647–658

    CAS  Google Scholar 

  • Gardner N, Champagne CP (2005) Production of Propionibacterium shermanii biomass and vitamin B12 on spent media. J Appl Microbiol 99:1236–1245

    Article  CAS  Google Scholar 

  • Haddadian MSY, Abu Reesh IM, Haddadian FAS, Robinson RK (2001) Utilisation of tomato pomace as a substrate for production of vitamin B12- a preliminary appraisal. Bioresour Technol 78:225–230

    Article  Google Scholar 

  • Hajfarajollah H, Mokhtarani B, Sharifi A, Mirzaei M, Afaghi A (2014) Toxicity of various kinds of ionic liquids towards the cell growth and end product formation of the probiotic strain, Propionibacterium freudenreichii. RSC Adv 4:13153–13160

    Article  CAS  Google Scholar 

  • Hugenschmidt S, Miescher Schwenninger S, Gnehm N, Lacroix C (2010) Screening of a natural biodiversity of lactic and propionic acid bacteria for folate and vitamin B12 production in supplemented whey permeate. Int Dairy J 20:852–857

    Article  CAS  Google Scholar 

  • Khuri AI, Cornell JA (1987) Response surfaces: design and analyses. Dekker, New York

    Google Scholar 

  • Kosmider A, Białas W, Kubiak P, Drozdzynska A, Czaczyk K (2012) Vitamin B12 production from crude glycerol by Propionibacterium freudenreichii sp. shermanii: optimization of medium composition through statistical experimental designs. Bioresour Technol 105:128–133

    Article  CAS  Google Scholar 

  • Li KT, Liu DH, Chu J, Wang YH, Zhuang YP, Zhang SL (2008a) An effective and simplified pH-stat control strategy for the industrial fermentation of vitamin B12 by Pseudomonas denitrificans. Bioprocess Biosyst Eng 31:605–610

    Article  CAS  Google Scholar 

  • Li KT, Liu DH, Li YL, Chu J, Wang YH, Zhuang YP, Zhang SL (2008b) Improved large-scale production of vitamin B12 by Pseudomonas denitrificans with betaine feeding. Bioresour Technol 99:8516–8520

    Article  CAS  Google Scholar 

  • Lofty WA, Ghanem KM, El-Helow ER (2007) Citric acid production by a novel Aspergillus niger isolate: II. Optimization of process parameters through statistical experimental designs. Bioresour Technol 18:3470–3477

    Google Scholar 

  • Martens JH, Barg H, Warren MJ, Jahn D (2002) Microbial production of vitamin B12. Appl Microbiol Biotechnol 58:275–285

    Article  CAS  Google Scholar 

  • Marwaha SS, Sethi RP (1984) Utilization of dairy waste for vitamin B12 fermentation. Agric Wastes 9:111–130

    Article  CAS  Google Scholar 

  • Marwaha SS, Sethi RP, Kennedy JF (1983) Influence of 5,6-dimethylbenzimidazole (DMB) on vitamin B12 biosynthesis by strains of Propionibacterium. Enzym Microb Technol 5:361–364

    Article  CAS  Google Scholar 

  • Miyano K, Ye K, Shimizu K (2000) Improvement of vitamin B12 fermentation by reducing the inhibitory metabolites by cell recycle system and a mixed culture. Biochem Eng J 6:207–214

    Article  CAS  Google Scholar 

  • Pereira FB, Guimarães PMR, Teixeira JA, Domingues L (2010) Optimization of low-cost medium for very high gravity ethanol fermentations by Saccharomyces cerevisiae using statistical experimental designs. Bioresour Technol 101:7856–7863

    Article  CAS  Google Scholar 

  • Piao Y, Yamashita M, Kawaraichi N, Asegawa R, Ono H, Murooka Y (2004) Production of vitamin B12 in genetically engineered Propionibacterium freudenreichii. J Biosci Bioeng 98:167–173

    Article  CAS  Google Scholar 

  • Placket RL, Burman JP (1946) The design of optimum multifunctional experiments. Biometrical 33:305–325

    Article  Google Scholar 

  • Quesada-Chanto A, Afschar AS, Wagner F (1994) Microbial production of propionic acid and vitamin B12 using molasses or sugar. Appl Microbiol Biotechnol 41:378–383

    CAS  Google Scholar 

  • Sen R, Swaminathan T (1997) Application of response-surface methodology to evaluate the optimum environmental conditions for the enhanced production of surfactin. Appl Microbiol Biotechnol 47:358–363

    Article  CAS  Google Scholar 

  • Suwannakham S, Huang Y, Yang ST (2006) Construction and characterization of ack knock-out mutants of propionibacterium acidipropionici for enhanced propionic acid fermentation. Biotechnol Bioeng 94(2):383–395

    Article  CAS  Google Scholar 

  • Wang P, Wang Y, Liu Y, Shi H, Su Z (2012) Novel in situ product removal technique for simultaneous production of propionic acid and vitamin B12 by expanded bed adsorption bioreactor. Bioresour Technol 104:652–659

    Article  CAS  Google Scholar 

  • Wyk JV, Witthuhn RC, Britz TJ (2011) Optimization of vitamin B12 and folate production by Propionibacterium freudenreichii strains in kefir. Int Dairy J 21:69–74

    Article  Google Scholar 

  • Yamada Y, Katsura K, Kawasaki H, Widyastuti Y, Saono S, Seki T, Uchimura T, Komagata K (2000) An unusual acetic acid bacterium in the al a-Proteobacteria. Int J Evol Microbiol 50:23–829

    Google Scholar 

  • Yang ST, Huang V (1995) A novel recycle batch immobilized cell bioreactor for propionate production from whey lactose. Biotechnol Bioeng 45(5):379–386

    Article  CAS  Google Scholar 

  • Ye K, Shijo M, Jin S, Shimizu K (1996) Efficient production of vitamin B12 from propionic acid bacteria under periodic variation of dissolved oxygen concentration. J Ferment Bioeng 82(5):484–491

    Article  CAS  Google Scholar 

  • Zhang A, Yang ST (2009) Engineering Propionibacterium acidipropionici for enhanced propionic acid tolerance and fermentation. Biotechnol Bioeng 104(4):766–773

    CAS  Google Scholar 

  • Zhang Y, Liu J, Huang J, Mao Z (2010) Genome shuffling of Propionibacterium shermanii for improving vitamin B12 production and comparative proteome analysis. J Biotechnol 148:139–143

    Article  CAS  Google Scholar 

  • Zulfiqar AR, Saleem Khan M, Khalid ZM (2007) Evaluation of distant carbon sources in biosurfactant production by a gamma ray-induced Pseudomonas putida mutant. Process Biochem 42:686–692

    Article  Google Scholar 

Download references

Acknowledgments

The authors would like to thank the Iran National Science foundation (INSF) for financial support of this research.

Author information

Authors and Affiliations

  1. Chemistry and Chemical Engineering Research Center of Iran, Tehran, Iran, P.O. Box 14335-186

    Hamidreza Hajfarajollah, Babak Mokhtarani, Hamidreza Mortaheb & Ali Afaghi

Authors
  1. Hamidreza Hajfarajollah
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Babak Mokhtarani
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hamidreza Mortaheb
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ali Afaghi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Babak Mokhtarani.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(DOCX 803 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hajfarajollah, H., Mokhtarani, B., Mortaheb, H. et al. Vitamin B12 biosynthesis over waste frying sunflower oil as a cost effective and renewable substrate. J Food Sci Technol 52, 3273–3282 (2015). https://doi.org/10.1007/s13197-014-1383-x

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-014-1383-x

Keywords

Search

Navigation