Abstract
The neutraceutical and pharmaceutical application of essential fatty acids is much cleared. Alpha-linolenic acid (ALA) is omega-3 fatty acid and generally known to have beneficial effects in CVS, CNS and other diseases. The purpose of the present investigation is to produce essential fatty acid, especially ALA by Mucor circinelloides from oil wastes. Five oil wastes collected from food industries were used as carbon sources, and the contents of total lipids, biomass and fatty acids were examined during 168 h. The ability of oil waste degradation was determined by measuring of biochemical oxygen demand (BOD) and chemical oxygen demand (COD). Interestingly, 76 % reduction in BOD and 68 % reduction in COD by this strain were achieved, and M. circinelloides could be a good candidate for oil waste treatment. In order to enhance ALA production, fermentation variables were chosen in accordance with the fractional design and further optimized by the response surface method. The statistical model was constructed via central composite design. Following the optimization step, ALA production increased by approximately 44.3 %, when compared to the screening step. The results indicate that carrying out the fermentation under the conditions of oil waste 4.37 %, yeast extract at 0.65 g/l, (NH4)2SO4 at 0.38 g/l, an agitation rate of 180 rpm and fermentation time of 3 days will increase the ALA production up to 108.57 mg/l. In this study, a new renewable source of ALA was employed and optimized successfully for the production of valuable fatty acids.
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References
Amaral C, Marcos L, Sampaio A, Peres J, Dias AA, Peixoto F, Anjos M, Pais C (2012) Biodegradation of olive mill wastewaters by a wild isolate of Candida oleophila. Int Biodeterior Biodegrad 68:45–50
Aminah S, Aidil SA, Wan Mohtar WY (2006) Medium optimization for the production of lipidless biomass by Cunninghamella sp. 2A1 using response surface methodology. Malays J Microbiol 2:40–45
Avijit H, Tripathi SK, Ghosh A (1999) Pharmacology and therapeutic potential of the n-3 polyunsaturated fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in fish oils. Indian J Pharmacol 31:247–264
Box GEP, Hunter SW, Hunter JS (1978) Statistic for experiments an introduction to design, data analysis and model building. Wiley, New York
Burdge GC, Calder PC (2005) Conversion of alpha-linolenic acid to longer-chain polyunsaturated fatty acids in human adults. Reprod Nutr Dev 45:581–597
Certik M, Shimizu S (1999) Biosynthesis and regulation of microbial polyunsaturated fatty acid production. J Biosci Biotechnol 87:1–14
Chang CY, Lue MY, Pan TM (2006) Statistical optimization of medium components for the production of Antrodia cinnamomea AC0623. Appl Microbiol Biotechnol 72:654–661
Chapman DJ, De-Felice J, Barber J (1983) Growth temperature effects on thylakoid membrane lipid and protein content of pea chloroplasts 1. Plant Physiol 72:225–228
Chatzifragkou A, Fakas S, Galiotou-anayotou M, Komaitis M, Aggelis G, Papanikolaou S (2010) Commercial sugars as substrates for lipid accumulation in Cunninghamella echinulata and Mortierella isabellina fungi. Eur J Lipid Sci Technol 112:1048–1057
Christie WW (1993) Preparation of ester derivatives of fatty acids for chromatographic analysis. In: Christie WW(ed) Gas chromatography and lipids. Oily Press, Dundee, pp 69–111
Clesceri LS, Greenberg AE, Eaton AD (1998) Standard methods for the examination of water and wastewater. American Public Health Association (APHA), Washington, DC
Devi P, Divy A, Shridhar MP, Dsouza L, Naik CG (2006) Cellular fatty acid composition of marine derived fungi. Indian J Mar Sci 35:359–363
Dyaneshwar W, Kalpana J, Abhay H (2006) Polyunsaturated fatty acids: biotechnology. Crit Rev Biotechnol 26:83–93
Fakas S, Certik M, Papanikolaou S, Aggelis G, Komaitis M, Galiotou- Panayotou M (2008) γ-Linolenic acid production by Cunninghamella echinulata growing on complex organic nitrogen sources. Bioresour Technol 99:5986–5990
Gill I, Valivety R (1997) Polyunsaturated fatty acids, part 1: occurrence, biological activities and applications. Trends Biotechnol 15:401–409
Haaland PD (1990) Experimental design in biotechnology. Elsevier Science, New York
Jangbua P, Laoteng K, Kitsubun P, Nopharatana M, Tongta A (2009) Gamma-linolenic acid production of Mucor rouxii by solid-state fermentation using agricultural by-products. Lett Appl Microbiol 49:91–97
Kendrick A, Ratledge C (1992) Lipids of selected moulds grown for production of n-3 and n-6 polyunsaturated acids. Lipids 27:15–20
Liang Y, Wen Z, Strait M (2012) Use of dry milling derived thin stillage for producing EPA by the fungus Pythium irregulare. Bioresour Technol 111:404–409
Linder M, Kochanowski N, Fanni J, Armentier M (2005) Response surface optimization of lipase catalyzed esterification of glycerol and n-3 polyunsaturated fatty acids from salmon oil. Process Biochem 40:273–279
Mamatha SS, Ravi R, Venkateswaran G (2008) Medium optimization of gamma linolenic acid production in Mucor rouxii CFR-G15 using RSM. Food Bioprocess Technol 1:405–409
Nakahara T, Yokochi Y, Higashihara T, Tanaka S, Yaguchi T, Honda D (1996) Production of docosahexaenoic and docosapentaenoic acid by Schizochytrium sp. isolated from Yap Islands. J Am Oil Chem Soc 73:1421–1426
Pan L, Yang D, Shao L, Li W, Chen G, Liang Z (2009) Isolation of oleaginous yeasts. Food Technol Biotechnol 47:215–220
Papanikolaou S, Sarantou S, Komaitis M, Aggelis G (2004) Repression of reserve lipid turnover in Cunninghamella echinulata and Mortierella isabellina cultivated in multiple-limited media. J Appl Microbiol 97:867–875
Papanikolaou S, Galiotou-Panayotou M, Fakas S, Aggelis G (2007) Lipid production by oleaginous Mucorales cultivated on renewable carbon sources. Eur J Lipid Sci Technol 109:1060–1070
Ratledge C (1992) Microbial lipids: commercial realities or academic curiosities. In: Kyle DJ, Ratledg C (eds) Industrial applications of single cell oils. AOCS, IL, pp 1–15
Rocky-Salimi K, Hamidi-Esfahani H, Abbasi S (2011) Statistical optimization of arachidonic acid production by Mortierella alpine CBS 754.68 in submerged fermentation. Iran J Biotechnol 9:87–95
Simpoulos AP (2002) The importance of the ratio of omega 6/omega 3 essential fatty acids. Biomed Pharmacother 56:365–379
Strong PG (2009) Fungal remediation of Amarula distillery wastewater. World J Microbiol Biotechnol 26:133–144
Tauk-Tornisielo S, Arasato LS, Almeida A, Govone JS, Malagutti EN (2009) lipid formation and g-linolenic acid production by Mucor circinelloides and Rhizopus sp., grown on vegetable oil. Braz J Microbiol 40:342–345
Vigh L, Escriba PV, Sonnleitner A, Sonnleitner M, Piotto S, Maresca B, Horvath I, Harwood JL (2005) The significance of lipid composition for membrane activity: new concepts and ways of assessing function. Prog Lipid Res 44:303–344
Yongmanitchai W, Ward OP (1989) Omega-3 fatty acids: alternative sources of production. Process Biochem 24:117–125
Zhu M, Yu L, Wu Y (2003) An inexpensive medium for production of arachidonic acid by Mortierella alpina. J Ind Microbiol Biotechnol 30:75–79
Acknowledgments
The authors would like to thank Dr Parvaneh Rahimi-Moghaddam M.D., Ph.D., Department of Pharmacology, College of Medicine, Iran University of Medical Sciences, and Gholamreza Ghezelbash and Hossein Ghanavati Ph. D students in University of Isfahan for their guidance and University of Isfahan for financially supporting this project.
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Mirbagheri, M., Nahvi, I., Emamzadeh, R. et al. Oil wastes management: medium optimization for the production of alpha-linolenic acid in Mucor circinelloides . Int. J. Environ. Sci. Technol. 13, 31–38 (2016). https://doi.org/10.1007/s13762-015-0849-3
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DOI: https://doi.org/10.1007/s13762-015-0849-3