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Insect cell culture as a tool in plant virus research: a historical overview

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Abstract

Culturing insect cells in vitro has witnessed remarkable growth in the past decades. Over 600 cell lines of dipteran, hemipteran, and lepidopteran insects have been reported so far. Besides its wide adoption for the production of recombinant proteins, viral pesticides, and vaccines, insect cell culture is increasingly being used as a tool for basic research in genetics, molecular biology, biochemistry, and virology. Since its first application in plant virus studies in 1956, insect cell culture has become an indispensable tool to study the complex and intimate interactions of plant viruses and their insect vectors. The in vitro cell cultures of several insect vectors of plant viruses including leafhoppers, planthoppers, aphids, thrips, and whiteflies have been successfully employed to understand the functions of viral proteins and receptor-mediated endocytosis into vector cells. This review summarizes the development of novel media and insect cell culture systems and their applications in plant virus research.

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Acknowledgments

The support received from Indian Agricultural Research Institute, DBT (BT/PR26136/AGIII/103/1005/2018), and SERB (EMR/2017/000590) is thankfully acknowledged. This research was also jointly supported by the Queensland Department of Agriculture and Fisheries and the University of Queensland through the Queensland Alliance for Agriculture and Food Innovation.

Author contribution statement

AG conceived and designed the review. HD and AG read through the related publications and wrote the draft manuscript. RKJ, RGD critically reviewed the manuscript and suggested edits; RGD assisted with English language editing. All authors read and approved the manuscript.

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Authors and Affiliations

  1. Insect Vector Laboratory, Advanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, 1100012, India

    Amalendu Ghosh, Heena Dhall & Rakesh Kumar Jain

  2. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD, 4072, Australia

    Ralf G. Dietzgen

Authors
  1. Amalendu Ghosh
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  2. Heena Dhall
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  3. Ralf G. Dietzgen
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  4. Rakesh Kumar Jain
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Correspondence to Amalendu Ghosh.

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Ghosh, A., Dhall, H., Dietzgen, R.G. et al. Insect cell culture as a tool in plant virus research: a historical overview. Phytoparasitica 48, 287–303 (2020). https://doi.org/10.1007/s12600-020-00795-7

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  • DOI: https://doi.org/10.1007/s12600-020-00795-7

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