Impact of food processing and detoxification treatments on mycotoxin contamination
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Mycotoxins are fungal metabolites commonly occurring in food, which pose a health risk to the consumer. Maximum levels for major mycotoxins allowed in food have been established worldwide. Good agricultural practices, plant disease management, and adequate storage conditions limit mycotoxin levels in the food chain yet do not eliminate mycotoxins completely. Food processing can further reduce mycotoxin levels by physical removal and decontamination by chemical or enzymatic transformation of mycotoxins into less toxic products. Physical removal of mycotoxins is very efficient: manual sorting of grains, nuts, and fruits by farmers as well as automatic sorting by the industry significantly lowers the mean mycotoxin content. Further processing such as milling, steeping, and extrusion can also reduce mycotoxin content. Mycotoxins can be detoxified chemically by reacting with food components and technical aids; these reactions are facilitated by high temperature and alkaline or acidic conditions. Detoxification of mycotoxins can also be achieved enzymatically. Some enzymes able to transform mycotoxins naturally occur in food commodities or are produced during fermentation but more efficient detoxification can be achieved by deliberate introduction of purified enzymes. We recommend integrating evaluation of processing technologies for their impact on mycotoxins into risk management. Processing steps proven to mitigate mycotoxin contamination should be used whenever necessary. Development of detoxification technologies for high-risk commodities should be a priority for research. While physical techniques currently offer the most efficient post-harvest reduction of mycotoxin content in food, biotechnology possesses the largest potential for future developments.
KeywordsMitigation Natural toxins Physical methods Chemical treatment Biological detoxification Decontamination
Toxic secondary metabolites produced by fungi belong to the most toxic contaminants regularly occurring in a wide range of food commodities (Bennett and Klich 2003). Most countries responded to this threat by establishing and enforcing maximum levels for mycotoxins in food (European Commission 2006; van Egmond et al. 2007). Setting maximum levels is based on toxicity assessment and exposure data but it also takes supply and demand into account. Raw materials are usually tolerated to have higher contamination levels (except for products intended for direct human consumption) than finished products. The rationale behind this is a dilution effect when formulating with non-contaminated ingredients in preparation of the final product as well as of the potential mitigation effects due to processing. In both cases, the mycotoxin concentration in the finished product will be lower than in the raw material.
Major mycotoxins and their producers, affected crops, adverse health effects and guidance values
Major producing fungi
Main affected crops
Principal adverse effects
Health-based guidance value (HBGV)
Aflatoxins FB1, FB2, FG1, FG2; metabolite AFM1 in milk
Aspergillus parasiticus, A. flavus (JECFA 2001a)
Extremely potent toxins and genotoxic carcinogens (after metabolic converstion to 8,9-epoxides in the liver); classified as carcinogenic to humans, AFM1 as possibly carcinogenic to humans (EFSA 2007; IARC 2012; JECFA 1999, 2001a)
Because of carcinogencity, exposure should be kept as low as reasonably achievable. No official HBGV
Ochratoxin A (OTA)
Aspergillus alutaceus, Aspergillus carbonarius, Penicillium verrucosum (EFSA 2006)
Fumonisins B1, B2, and B3 (FB1, FB2, FB3)
Inhibit sphingolipid biosynthesis; induction of apoptosis, tumors in rodents (EFSA 2005; JECFA 2001a; SCF 2003, IARC 2002), putative teratogenicity; FB1 classified as possibly carcinogenic to humans (IARC 2002)
Deoxynivalenol (DON) and its acetylated derivates (3- and 15-acetyl-DON)
Feed refusal, vomiting, and diarrhea; reduced growth; thymus, spleen, heart, liver, and immune system affected at higher doses (EFSA 2004; IARC 1993; JECFA 2001a; SCF 2002); not classifiable as to carcinogenicity to humans, (IARC 1993)
Other trichothecenes, e.g., T-2 toxin, HT-2 toxin, nivalenol (NIV)
Cereals (EFSA 2011a)
Acute effects of T-2 similar to high dose radiation (diarrhea, hemorrhage, hematotoxicity, and immune suppression) (JECFA 2001a, EFSA 2011a); toxicological profile of NIV similar (EFSA 2013); not classifiable as to carcinogenicity to humans (IARC 1993)
TDI 1.2 μg/kg BW/day for NIV (EFSA 2013)
Gastrointestinal ulceration; immunotoxicity and neurotoxicity in animals; genotoxic (JECFA 1996); inadequate evidence of carcinogenicity in animals, not classifiable as to its carcinogenicity to humans (IARC 1986)
PMTDI 0.4 μg/kg BW/day (JECFA 1996)
Interact with neurotransmitter receptors; acute toxicity: convulsive neurotroxicity, uterine hemorrhage, and abortions; chronic toxicity: vasoconstriction with ischemia and necrosis of extremities (ergotism) (EFSA 2012, BfR 2004)
Various EAs seem to have similar toxic potency; group ARfD 1 μg/kg BW/day and group TDI 0.6 μg/kg BW/day; both apply to the sum of EAs (EFSA 2012)
Harmful effects of mycotoxin-contaminated food can be avoided by (i) preventing contamination, (ii) removing contaminated material from the food commodity, (iii) mitigating mycotoxin content in food, and (iv) treating exposed individuals. In some commodities, only part of the harvest enters the food chain. Selection of charges with low mycotoxin levels for consumption while using the remainder for feed and energy production would reduce the exposure of consumers to mycotoxins. Unfortunately, this is only possible in a few commodities and, even there, production systems targeting food markets, feed manufacturing, and energy production are often so specialized that they cannot replace each other. The first priority therefore remains prevention of toxin accumulation directly on the field (preharvest) or thereafter (transport and storage) (Kabak et al. 2006; Choudhari and Kumari 2010). A variety of agricultural practices, e.g., growing resistant crop varieties, crop rotation, soil tillage, chemical and biological control of plant diseases, and insect control are available to minimize mycotoxin production on the field (Edwards 2004; Munkvold 2014; Mesterhazy 2014; Alberts et al. 2016). Proper harvest and storage conditions are crucial to prevent fungal growth and mycotoxin accumulation in harvested commodities (Jacobsen 2014). Unfortunately, preharvest measures do not guarantee the absence of mycotoxins in food or feed. Food processing can impact mycotoxins in raw material by (i) physical removal, (ii) chemical transformation which can result in metabolites of lower or higher toxicity, (iii) release from masked or entrapped forms which may increase bioavailability, (iv) enzymatic detoxification, and (v) adsorption to solid surfaces. Physical and chemical mechanisms reducing mycotoxin content often act together in the same food processing step. For instance, sulfur dioxide used in corn wet milling to ease the separation of germs, proteins, and starch possesses potential for chemical detoxification. Reduction of mycotoxin contamination was documented for cleaning; milling; brewing; fermentation; cooking; baking; frying; roasting; flaking; alkaline cooking; nixtamalization (soaking, cooking in an alkaline solution, and hulling of grains); and extrusion. Concentrations of some mycotoxins can be reduced substantially while others, such as DON, are relatively resistant to degradation (Milani and Maleki 2014; Karlovsky 2011). Detoxification of grain mycotoxins during food processing has recently been reviewed (Kaushik 2015). As the last resort, consumers can be prophylactically treated with binders in areas of chronically high aflatoxin exposure (Afriyie-Gyawu et al. 2008; Wang et al. 2008).
The following terms are used to describe the outcome of mitigation treatments throughout this article: removal of mycotoxins from raw materials and/or finished products, transformation (modification of the chemical structure of the molecule), detoxification (transformation which reduced the toxicity), and decontamination (removal or detoxification/inactivation). Effective decontamination should be irreversible, modified forms of mycotoxins should be affected together with parent compounds, the products should be non-toxic, and the food should retain its nutritive value and remain palatable (Milani and Maleki 2014). Processing procedures, agents, and microorganisms must be allowed for use in food (Codex Alimentarius, 2015). The interested reader is also referred to European Commission Regulation 2015/786, defining acceptability criteria for detoxification processes applied to products intended for animal feed (EC 2015). These criteria may serve as a model for the assessment of mycotoxin detoxification technologies in food processing. Compliance of a given detoxification process with those criteria will be assessed by the European Food Safety Authority (EFSA).
In this review, conventional food processing affecting mycotoxins as well as processes dedicated to decontamination are covered. Applications of the techniques to selected commodities are presented for illustration, knowledge gaps are outlined, and recommendations for prioritizating mitigation actions and further research are given.
Physical processing methods
Aflatoxin contamination is usually heterogeneous so that separating damaged kernels can effectively reduce contamination (Kabak et al. 2006). Grain sorting using UV light illumination for aflatoxin reduction is common. The observed bright greenish-yellow fluorescence (BGYF) does not originate from aflatoxins but from a kojic acid derivative following reaction with endogenous peroxidase. In dried commodities, peroxidase is inactivated and the BGYF method does not work. The quick and easy “black light test” may therefore result in both false positive and false negative findings (Bothast and Hesseltine 1975). Although the test is not as reliable as originally hoped (Doster and Michailides 1998), it is widely used, e.g., by Turkish companies exporting dry figs and nuts to the EU. As an audition by the Food and Veterinary Office of the EU confirmed, the efficiency of sorting is regularly verified by laboratory analysis (EC 2013).
Distribution of ergot alkaloids (EAs) is even more heterogeneous than aflatoxins because intermediate contamination does not exist at a single-kernel level. Sclerotia loaded with EAs are efficiently removed from rye by opto-electronic sorting (Young et al. 1983; Miedaner and Geiger 2015).
Because infection with Fusarium verticillioides often does not cause symptoms (Munkvold and Desjardins 1988) and correlation between fumonisin content and symptoms is weak (Afolabi et al. 2007), grain sorting might not reduce fumonisin content efficiently though successful attempts have been reported (Pearson et al. 2004). Mycotoxins accumulating without visible symptoms pose a limit to optical sorting as a mycotoxin mitigation strategy. This may explain why no reduction of aflatoxin content by sorting was found in a recent study (Mutiga et al. 2014).
Removing kernels with extensive mold growth, broken kernels, and fine materials such as dirt and debris can be achieved by sieve cleaning, which significantly lowers total mycotoxin contamination. Removal of EAs from wheat grains by sieving has been used as a plant quarantine treatment (Muthaiyan 2009). After sieving off corn screenings, it was determined that intact kernels contained about 10 times less fumonisins than broken corn kernels or smaller parts (Murphy et al. 1993). Particles passing through a 3-mm sieve usually constitute 5–20 % of a sample by mass, but contain 26–69 % of fumonisins (Sydenham et al. 1994). Removing broken kernels and smaller parts from maize reduced DON and ZEN contamination by around 70–80 %; however, up to 69 % of the total maize was rejected as well (Trenholm et al. 1991). Losses of barley or wheat were lower with 34 and 55 % for comparable mycotoxin reduction.
Flotation and density segregation
The different physical properties of mold-damaged kernels compared to non-damaged kernels can be exploited to separate them by density segregation or by fractionation on gravity tables. The damage to the kernels is caused by different fungi, rendering this techniques sensitive to the overall fungal contamination, rather than to specific toxins. Ergot containing EAs can be efficiently separated from rye grains by flotation in NaCl solution (Plante and Sutherland 1944). Removal of corn buoyant in water reduced aflatoxin levels by 60 %, at a mass loss of 22 % (Huff 1980). Using a 30 % sucrose solution, 87 % aflatoxin reduction was achieved by the removal of 50 % of the material (Huff 1980). Flotation on a saturated sodium chloride solution removed not only 3 % of the kernels but also 74 % of the total aflatoxin content in maize (Huff and Hagler 1985). Likewise, the removal of kernels floating on both water and 30 % sucrose reduced more than 53 % DON in maize and above 68 % in wheat. The same procedure basically removed all ZEN in the tested samples (Huff and Hagler 1985). In a later study, fumonisin reduction of 86 % was achieved by removing maize kernels buoyant in saturated brine, with about 20 % material loss (Shetty and Bhat 1999).
Hydrophobicity (log P) and solubility in water (in mg/l) of selected mycotoxins. Given data were gathered from databases using predictive tools
predicted log P*
Predicted solubility in water (mg/l)*
Entry number in the toxin and toxin target database (T3DB)
The outer layers of grain are removed by dehulling techniques, which are formerly composed of an indispensable processing step prior to grinding. Limitation of fungal colonization and mycotoxin accumulation to surface layers of the kernel are prerequisites for the success of dehulling in mycotoxin content reduction (Vučković et al. 2013). This condition is fulfilled for aflatoxins in maize, dehulling of maize can therefore remove up to 93 % of aflatoxins (Siwela et al. 2005). During the preparation of muthokoi, a traditional dehulled maize dish in Kenya, aflatoxin content was reduced by 46.6 % (Mutungi et al. 2008), leading to significantly lower dietary exposure due to maize meal and muthokoi consumption as compared to the consumption of entire kernels (Kilonzo et al. 2014).
This is the first step in wet milling of maize and involves soaking maize for 36–50 h at 50 °C in water containing 0.1 to 0.2 % SO2 to facilitate germ separation and breaking down of protein matrix. Adding SO2 also promotes lactic acid production which can be regarded as a chemical treatment from this review’s perspective. Half of the aflatoxin content of corn was found in the steep liquor (Aly 2002). Fumonisins, which are highly polar, migrate from kernels into steeping water (Canela et al. 1996). Pujol et al. (1999) reported that steeping corn kernels in 0.2 % solution of SO2 at 60 °C for 6 h was effective in reducing FB1. OTA was distributed equally between solubles and corn grits (Wood 1982). Steeping sorghum grains in 0.2 % NaOH reduced the concentration of aflatoxins, fumonisins, ZEN, and DON under detectable levels (Lefyedi and Taylor 2006).
After milling small-grain cereals, high mycotoxin levels are found in bran, such as DON, while finished flour is contaminated to a much lower degree (Cheli et al. 2013; Tibola et al. 2015). Spatial distribution of DON, ZEN, and their masked forms in wheat milling fractions was studied by Schwake-Anduschus et al. (2015). ZEN was concentrated in fiber-rich parts of grains DON contaminated all fractions equally, showing that the efficiency of milling as a mycotoxin mitigation strategy is limited to commodity/mycotoxin pairs in which mycotoxins are enriched in fractions that can be removed from processing.
Wet milling of maize results in germ (further processed into germ oil), starch, and gluten. Different mycotoxins accumulate in different fractions except for the starch fraction in which all mycotoxins are reduced below a level of concern. 40–50 % of aflatoxins moved from corn into steep water in wet milling, 28–38 % remained in the fiber fraction, 11–17 % in the gluten fraction, 6–11 % in the germ, and only 1 % in starch (Yahl et al. 1971; Bennett and Anderson 1978). Fumonisins are partly dissolved in steep water. At very high contamination levels, significant amounts of fumonisins remained in gluten and fiber. Germ fractions are less affected and starch is virtually free of fumonisins (Bennett et al. 1996). Two thirds of T-2 toxin were removed by steep and process water during wet milling of maize, starch contained 4 % of the toxin and the remainder was evenly distributed between germ, gluten, and fiber (Collins and Rosen 1981). OTA present in steeped corn (see earlier) went into process water solubles and corn grits in almost equal amounts while only 4 % were transferred to the germ (Wood 1982).
Dry milling of maize grain leads to concentration of mycotoxins in germ and bran fractions (Bullerman and Bianchini 2007). Aflatoxins are concentrated in germ fraction to a higher degree than fumonisins (Pietri et al. 2009). Increased concentrations of 16 Fusarium spp. mycotoxins in bran and germ as compared to whole grain were reported for dry-milled maize by Schollenberger et al. (2008). As expected, apolar ZEN was mainly found in germ and bran fractions after dry milling (Bennett et al. 1976). Milling results in redistribution of the ergot sclerotia with EAs among milling fractions (EFSA 2012).
The time/temperature combination undoubtedly remains one of the most important interventions by which industrial processing can affect the mycotoxin content in a finished food product. Most mycotoxins are chemically and thermally stable though. While conventional food preparation with temperatures up to 100 °C have little effect on most mycotoxins, higher temperatures used in frying, roasting, toasting, and extrusion might reduce mycotoxin contamination.
Aflatoxins can be reduced by extrusion by 50–80 %, depending on grain moisture and temperature (Bullerman and Bianchini 2007). Alkaline treatment (see next chapter) can increase the efficacy of this process. Similar results were achieved for peanut meal, when extrusion alone reduced aflatoxins by 23–66 % and up to 87 % in the presence of ammonium hydroxide (Cheftel 1989). Roasting can reduce the levels of aflatoxins by 50–70 % in peanuts and pecans and by 40–80 % in maize (Conway et al. 1978). Pure aflatoxin B1 (AFB1) was destroyed by temperatures above 160 °C; soybean matrix accelerated the process (Raters and Matissek 2008). Roasting can reduce the content of OTA in coffee beans by up to 97 %, depending on the temperature and particle size (Oliveira et al. 2013). Degradation of OTA in wheat by heating (Boudra et al. 1995) and extrusion (Scudamore et al. 2004) was less efficient. Thermal degradation products of OTA are 14-(R)-ochratoxin A, 14-decarboxy-ochratoxin A and ochratoxin alpha amide, all of which have reduced toxicity (Cramer et al. 2008; Bittner et al. 2015). Cazzaniga et al. (2001) reported drastic reduction of the level of DON under all investigated conditions but other labs found moderate effects depending on the conditions (Wu et al. 2011) or no reduction of DON and nivalenol (NIV) (Scudamore et al. 2008). Extrusion cooking of maize grits contaminated with ZEN reduced the toxin content by 65–83 % (Ryu et al. 1999). Extrusion or roasting was also effective in reducing fumonisins in maize grits by 34–95 % (Bullerman and Bianchini 2007). Increased temperature, decreased screw speed, and glucose addition resulted in higher reduction rates during extrusion. Thermal treatment always involves transformation reactions. Fumonisin in corn extruded with glucose (Bullerman and Bianchini 2007) yielded N-(1-deoxy-d-fructos-1-yl)-fumonisin B1, a compound less toxic than fumonisin B1 to rats (Hahn et al. 2015). Citrinin (CIT) could also be efficiently degraded by heating (Trivede et al. 1992). EAs are partly degraded and epimerized during bread baking; the ratio between epimers shifts towards the -inine forms (EFSA 2012; Merkel et al. 2012).
Irradiation may be an approach for removing mycotoxins on an industrial scale, providing in fact energy to both food constituents and contaminants: reactions occur and change the molecular structure of food constituents. Non-ionizing (solar, UV, microwave) and ionizing (gamma) radiations can reduce or eliminate pathogenic microorganisms, but partly also mycotoxins in food.
Photodegradation of aflatoxins in cereals has been found to decrease toxin levels by about 40 % after 3 h and up to 75 % after 30 h of direct sunlight (Herzallah et al. 2008). The same authors found sunlight to be more effective than 10 min of microwave heating (32 % reduction) or gamma-irradiation with 25 kGy (43 % reduction). In another study, peanuts, pistachios, rice, and corn were irradiated with gamma radiation (Ghanem et al. 2008). At higher doses, the aflatoxin reduction was pronounced, reaching 59–88 % at 10 kGy. However, the most recent study showed aflatoxin reductions of only 11–21 % at 15 kGy (di Stefano et al. 2014). The same study reported the reduction of OTA in almonds to 24 % by gamma radiation at 15 kGy. This number is in good agreement with fumonisin reduction in maize, which was found to be about 20 % after a dose of 15 kGy (Visconti et al. 1996). Microwave treatment (and convection heat) was partially successful in lowering DON levels in naturally contaminated maize, with greatest effect occurring at the highest temperatures. With final temperatures of 150–175 °C, a 40 % reduction was achieved (Young 1986). This reduction can be explained by the formation of several nor-DONs, which are far less cytotoxic than DON (Bretz et al. 2006). Exposure of vegetable oil contaminated with ZEN to sunlight through common glass bottles caused isomerization of natural trans-ZEN into cis-ZEN with a conversion of up to 90 % (Köppen et al. 2012). UV light is very effective in removal of PAT in apple juice and cider. Evaluation of the reduction of PAT in apple juice at different wavelengths in the UVC range showed that 222 nm was most suitable (Zhu et al. 2014). At an initial level of about 1000 μg/l, the UV exposure was successful in reducing PAT levels by 5 to 73 %, depending on the number of passes (Assatarakul et al. 2012). UV exposure, however, affected the taste of apple juice and cider.
Cold plasma has strong antimicrobial effects and can be used to sterilize fragile or temperature-sensitive surfaces, such as food. A recent review on the use of plasma for food processing (Schlüter et al. 2013) highlighted the potential of this new technique that at the same time demands cautious use. No investigation on potential formation of toxic compounds by plasma treatment has been conducted yet. The authors concluded that plasma-treated products have to be assessed on a case by case basis for the time being.
Low-pressure cold plasma destroyed up to 50 % of alfatoxins on nut surfaces (Basaran et al. 2008). The effect of atmospheric pressure argon cold plasma on spores and mycotoxin production of Aspergillus niger contaminating date palm fruits was recently evaluated (Ouf et al. 2015). After treatment for 9 min, all fungal spores were killed, OTA and fumonisin B2 contents dropped from 25 and 6 μg/100 mm2, respectively, below the limits of detection. Cold plasma generated by atmospheric dielectric barrier discharge in a direct and remote mode with synthetic air as working gas reduced the concentration of DON and ZEN in thin layers from 100 μg/ml to a few micrograms per milliliter (ten Bosch et al. 2014).
Mycotoxin binders are a physical technique used for feed decontamination (Jans et al. 2014) that principally can also be used in human intervention. Activated charcoal was used to remove patulin from naturally contaminated cider and bentonite removed AFM1 from naturally contaminated milk (Doyle et al. 1982). De Nijs et al. (2012) discussed the efficiency of mycotoxin mitigation and food safety aspects of such techniques. The efficiency of binders in mitigating adverse effects of aflatoxins in food was demonstrated in a randomized and double-blinded clinical trial (Wang et al. 2005; Afriyie-Gyawu et al. 2008; Wang et al. 2008). These are the only reports on the use of such techniques in food so far.
Chemical processing methods
Many studies investigated chemical food processing methods for their suitability to destroy or inactivate mycotoxins. It should be noted that chemical treatment for the purpose of detoxification or decontamination is not authorized within the EU for commodities destined for human food; dedicated mitigation treatments would therefore require regulatory approval. Chemicals transform mycotoxins into other compounds, the toxicity of which must be assessed. It is furthermore crucial that the treatment does not impair nutritional quality, texture, or flavor of food. Criteria recently defined for the approval of detoxification techniques for feeds (EC 2015) may serve as a model for the development of corresponding regulations for food.
Common food processing technologies may reduce mycotoxin content as a side effect of accompanying chemical processes. Codex Alimentarius provides a list of such chemicals (Codex Alimentarius General Standard for Food Additives) and national legislations regulate their use. In this section, the effect of chemical treatments on mycotoxins in food will be reviewed regardless of whether the treatments are part of standard food processing or they have been studied as dedicated detoxification methods. The use of chemicals in combination with physical treatments, described in the previous section, may increases the efficacy of mycotoxin degradation. Chemical agents for mycotoxin detoxification can be applied by mixing, packing, fumigation or immersion.
Most of the known mycotoxins are resistant against weak acids, as reviewed by Müller (1983). However, treatment of aflatoxins with strong acids destroyed the biological activity of AFB1 and AFG1 by converting them to hemiacetal forms AFB2a and AFG2a, respectively (Ciegler and Peterson 1968; Dutton and Heathcote 1968). Treatment with HCl (pH 2) reduced AFB1 levels by 19 % within 24 h (Doyle et al. 1982). In the presence of acetic anhydride and hydrochloric acid, the reaction proceeds further to give the acetoxy derivative. Similar adducts of AFB1 and AFG1 are formed with formic acid-thionyl chloride, acetic acid-thionyl chloride, and trifluoroacetic acid. Aiko et al. (2016) treated aflatoxins with diluted acetic acid, citric acid, and lactic acid under conditions simulating cooking. Lactic acid was most efficient, converting AFB1 into AFB2 (traces) and AFB2a (major product). Apart from detoxifying aflatoxins, small carboxylic acids inhibit mold growth, and are therefore used as preservatives.
Treatment with bases
Aflatoxins are unstable under alkaline conditions (Kiermeier and Ruffer 1974; Itoh et al. 1980), the first step of degradation being the opening of the lactone ring. Because this step is reversible, it is important to allow the reaction to proceed to completion. The degradation of aflatoxins in groundnut and cottonseed meal as well as in corn by sodium hydroxide and other alkaline reagents (Ca(OH)2, Na2CO3, Na3PO4, methylamine, ethylene-diamine, ethanolamine) has been reviewed by Müller (1983). In most cases, a partial detoxification was achieved. Degradation of aflatoxins using ammonia has been extensively studied and proved effective in laboratory experiments as well as in field trials effective (Müller 1983; Park et al. 1988). Ammoniation of AFB1 resulted in two major breakdown products which retained the difuran moiety but lost the lactone ring: aflatoxin D1 and D2 [4-hydroxy-6-methoxy-3a,8a-dihydrofuro[2,3]benzofuran] (Cucullu et al. 1976). Ammoniation decreased aflatoxin levels in maize by more than 75 % (Park et al. 1988) and completely decomposed OTA in maize, wheat, and barley (Chełkowski et al. 1981). Ammoniation can reduce aflatoxin concentration by more than 99 % (Chełkowski et al. 1981; Masri et al. 1969; Brekke et al. 1977). The efficacy of the process depends on the temperature, pressure, moisture, duration, and the substrate (Weng et al. 1994). Park et al. (1988) reviewed the decontamination of aflatoxins by ammoniation and concluded that the results demonstrate overwhelming support for the efficacy and safety of ammoniation as a practical solution to aflatoxin detoxification in oilseed meals intended for animal feeding. In spite of three decades of encouraging research, ammoniation of food commodities has, to our knowledge, not been approved in any country so far.
The instability of rubratoxin and cyclochlorotin under alkaline conditions was shown by Moss (1971) and Ishikawa et al. (1970), respectively. Chełkowski et al. (1981) demonstrated reduction of the content of penicillic acid, CIT, OTA, ZEN, and AFB1 after treatment with 2 % aqueous NH3. PAT was unstable already at pH 7, while at pH 8, it was degraded completely after 190 h (Brackett and Marth 1979). Bennett et al. (1980) described 80 % reduction of ZEN in spiked corn grits and 64 % reduction in naturally contaminated corn after treatment with 3 % NH3 at 50 °C for 16 h.
Treatment of FB1-contaminated corn with Ca(OH)2, simulating nixtamalization (soaking/cooking in an alkaline solution), completely hydrolyzed FB1 but the toxicity of the products in brine shrimp assay was only partly reduced (Park et al. 1996). Ammoniation reduces the concentration of FB1 in wheat by 79 % (Park et al. 1992) but was inefficient in corn according to Norred et al. (1991). Toxicity of the products of alkaline hydrolysis of fumonisins varies considerably among species (see enzymatic hydrolysis of fumonisins in the following section for details). Until these effects are fully understood, alkaline treatment of fumonisin-containing food ingredients should be avoided.
Use of oxidizing agents
The oxidation of aflatoxin appears to be primarily an addition directed towards the double bond of the terminal furan ring, followed by subsequent reactions involving the phenol formed on opening the lactone ring. It was well-known that aflatoxins such as AFB1, AFG1, and AFM1, which had a terminal double bond in the dihydrofuran ring were more susceptible to attack by ozone (O3) and other oxidizing agents than AFB2, AFG2, and AFM2, which lack this double bond (McKenzie et al. 1997). It is postulated that ozone reacts with the C8–C9 double bond of the furan ring of aflatoxin through electrophilic attack, causing the formation of primary ozonides followed by rearrangement into derivatives such as aldehydes, ketones, and organic acids (Proctor et al. 2004). Studies have demonstrated that ozone is able to degrade aflatoxins in different commodities (Dwarakanath et al. 1968) and in aqueous solutions (Maeba et al. 1988). AFB1 and AFG1 were sensitive to ozone and easily degraded with 1.1 mg/l of ozone within 5 min at room temperature. On the other hand, aflatoxins B2 (AFB2) and G2 (AFG2) were rather resistant to ozone, requiring 50–60 min to degrade them completely with 34.3 mg/l of ozone. These forms require longer exposure for detoxification; a possible mechanism is opening of the lactone ring (Samarajeewa et al. 1990).
Ozone was reported to reduce AFB1 and AFG1 levels by 77 and 80 %, respectively, in peanuts after treatment at 75 °C for 10 min, while the maximum degradation of 51 % was achieved for AFB2 and AFG2 regardless of the exposure time (Proctor et al. 2004). In another study, the reductions of AFB1 in paprika were 80 and 93 % after exposures to 33 and 66 mg/l O3 for 60 min, respectively (Inan et al. 2007). Ozone degradation has been shown to be effective also against other mycotoxins such as DON (Young 1986, Young et al. 1986) and moniliformin (Zhang and Li 1994). A highly concentrated ozone produced by an electrochemical method (Rogers et al. 1992) was able to degrade and detoxify several mycotoxins in vitro, including aflatoxins, cyclopiazonic acid, OTA, PAT, secalonic acid D, and ZEN after treatment with O3 at 10 % for 15 s (McKenzie et al. 1997).
Hydrogen peroxide, H2O2, was used on a commercial scale to detoxify aflatoxins. Treatment of figs with H2O2 at 0.2 % caused a 66 % reduction in AFB1 levels following 72-h storage (Altug et al. 1990). Hydrogen peroxide reduced aflatoxin concentrations in corn (Chakrabarti 1981), peanut meal (Sreenivasamurthy et al. 1967), and milk (Applebaum and Marth 1982). The concentration and the toxicity of ZEN decreased after treatment with aqueous solution of H2O2 (Lasztity et al. 1977). The efficiency of H2O2 for destruction of ZEN in contaminated corn was found to dependent upon the concentration of H2O2, temperature, and period of exposure (Abd Alla 1997). CIT was completely detoxified by 0.05 % H2O2 after 30 min whereas OTA was resistant to this treatment (Fouler et al. 1994).
Matsuura et al. (1979) reported that ZEN is destroyed by oxidation with ammonium persulfate. Natarajan et al. (1974) showed that sodium hypochlorite concentration and pH, but not temperature and time, affected the destruction of aflatoxins in peanut protein. Aflatoxin degradation by sodium hypochlorite was compared with the effect of sodium hydroxide and ammonium hydroxide (Draughon and Childs 1982). All three treatments significantly reduced fluorescence but the survival of brine shrimp has not always increased. The mutagenic and cancerogenic aflatoxin B1-2,3-dichloride may be formed using sodium hypochlorite, although this can be avoided by adding acetone (Castegnaro et al. 1981). Aflatoxins were removed completely from rice meal treated with 16.5 % NaCl and 1 % NaOCl for 24 h (Okonko and Nwokolo 1978).
Treatment with reducing agents
Sodium bisulfite (NaHSO3) was shown to destroy mycotoxins, primarily AFB1 in maize (Doyle et al. 1982) and dried figs (Altug et al. 1990). Dried fig fruits were spiked to contain 250 μg/kg AFB1 and treated with sodium bisulfite (1 % in the aqueous phase). This treatment caused 28 % of added toxin to degrade within 72 h at 25 °C. When 0.2 % H2O2 were added 10 min before the bisulfite treatment, 65 % of AFB1 were degraded in 72 h. Heating bisulfite-treated samples at 45 to 65 °C for 1 h caused up to 68 % of added AFB1 to be degraded. Promising results have been achieved in AFB1, AFG1, and AFM1 detoxification using sodium bisulfite (Doyle and Marth 1978a, b; Moerck et al. 1980; Hagler et al. 1982; Yagen et al. 1989). Moerck et al. (1980) and Hagler et al. (1982) demonstrated efficient destruction of low and high levels of aflatoxin in corn. The decontaminated corn had improved color, better palatability, better handling properties, improved economics, and the residual bisulfite was a permitted food additive. Yagen et al. (1989) established the structure of aflatoxin B1S as 15α-sodium sulfonate of AFB1. The formation of AFB1 products substituted at the 15th position only is unprecedented and implies an unusual mechanism. The completeness of the reaction and the water solubility of aflatoxin B1S supports the use of bisulfite as a promising method to mitigate AFB1 and AFG1.
NaHSO3 solutions reduced DON in contaminated maize (4.4 mg/kg) by 85 % after 18 h at 80 °C (Young et al. 1987). Sodium metabisulfite at 10 g/kg was reported to overcome the depressing effect of DON on feed intake in piglets (Dänicke et al. 2005). Reduction of DON in animal feed by treatment with sodium bisulfite and sodium metabisulfite has been demonstrated in several studies. The treatment leads to the formation of less toxic DON sulfonate; a review is available (Dänicke et al. 2012). Later, it was discovered that the use of different sulfur reagents for reduction of DON yielded three different DON sulfonates with the same mass and molecular formula (Schwartz et al. 2013). DON sulfonate 1 is characterized by loss of the epoxide group, and DON sulfonate 2 by formation of a hemiketal. DON sulfonate 3 is an equilibrating mixture of two isomers, a ketone and a hemiketal. Rapid formation of DON sulfonates 1 and 2 occurs at alkaline pH, slow formation of DON sulfonate 3 takes place at acidic pH, irrespective of the sulfur reagent used. Whereas DON sulfonates 1 and 2 are stable across a broad pH range, DON sulfonate 3 decomposes to DON and DON sulfonates 1 and 2 at alkaline pH (Schwartz et al. 2013).
Treatment with food ingredients and medical plants
Certain spices, herbs, and other ingredients used in food production and home cooking were shown to detoxify mycotoxins. Incubation with extract of ajwan (carom), used as a spice in Asian cooking, destroyed aflatoxins (Velazhahan et al. 2010). Extracts of medicinal plants Ocimum tenuiflorum detoxified aflatoxins even at room temperature (Panda and Mehta 2013) and aqueous extracts of vasaka leaves (Adhatoda vasica) degraded AFB1 completely after 24 h at 37 °C (Vijayanandraj et al. 2014). Detoxification of aflatoxins by Indian spices and herbs was recently reviewed (Aiko and Mehta 2015). Reducing sugars such as D-glucose and D-fructose blocked the primary amino group of FB1, after incubation at 65 °C for 48 h, preventing FB1-induced toxicity on cell tissue cultures on rats and swine (Fernandez-Surumay et al. 2005).
Distinguishing features of enzymatic detoxification
Enzymatic catalysis takes a unique position among activities potentially suitable to detoxify mycotoxins. A distinguishing feature of enzymatic detoxification is its specificity. Notable exceptions are laccases and peroxidases, which have been tested for degradation of mycotoxins (Alberts et al. 2009; Wang et al. 2011) though they modify a wide range of substrates and may thus destroy valuable food components. The potential of enzymatic activities for the detoxification of mycotoxins in general was recently reviewed (Vanhoutte et al. 2016) but only a single short review is available on the potentical of enzymatic detoxification of mycotoxins in food production (Karlovsky 2014).
Enzymes are proteins and, when used in food processing, may cause allergy. However investigation of the allergenic potential is part of the documentation required for the approval of enzymes as additives or processing aids (EFSA 2009; JECFA 2001b). No allergic reaction to current food enzymes has been reported so far, indicating that enzymes are of limited concern regarding food allergies (Bindslev-Jensen et al. 2006). Because of their specificity and favorable toxicological profile, enzymes possess a yet unexplored potential to detoxify organic contaminants in food. A recent application pointing the way is the use of recombinant asparaginase to prevent formation of acrylamide in bread (Hong et al. 2014). No enzyme has so far been authorized in the EU for the reduction of mycotoxin contamination in food.
Intentional use of enzymes to detoxify mycotoxins in food production
Enzymes are used extensively as processing aids. For instance, recombinant aspartic protease chymosin is an alternative to rennet in the manufacturing of cheese (Teuber 1993) and industrial enzymes of five classes are used in bread making (Whitehurst and van Oort 2010). Malting and brewing are further examples of processes that would benefit from the use of enzymes detoxifying mycotoxins. Beer is commonly contaminated with DON (see section “Ethanol and beer”). Adding enzymes detoxifying DON to amylases, glucanases, proteases, and other enzymes used in beer production (Whitehurst and van Oort 2010) is compatible with brewing technologies. Unfortunately, enzymes suitable for irreversible detoxification of DON are not available yet. Promising new detoxification activities have been identified (Ito et al. 2013; He et al. 2015); it remains to be seen whether enzymes responsible for these activities are suitable for industrial production.
DON is a major mycotoxin contaminating wheat. Because the same mycotoxin occurs in beer and wheat flour, enzymes envisaged for the detoxification of DON in beer might be suitable for bakery products, too. Such an enzyme could be added to wheat flour together with recombinant xylanases, proteases, amylases, and other enzymes commonly used in the process.
Another example of the potential of industrial enzymes to reduce exposure to mycotoxins is the detoxification of PAT. Fruit juices and particularly purees may contain PAT. Production of juices involves treatment with pectinases/arabanases, glucoamylases, and other enzymes. Enzymatic activities degrading PAT have been found in many species of bacteria and yeast (for instance, Zhu et al. 2015b). Degradation products are less toxic (Castoria et al. 2011; Zhu et al. 2015a), indicating that mitigation of PAT by enzymes is feasible. At least one of the enzymes detoxifying PAT does not require diffusible cofactors and is active in semi-purified form (Zhu et al. 2015a). Degradation of PAT can likely be combined with current enzymatic treatments used in the production of fruit juices and purees.
Widespread use of enzymes in food processing suggests that detoxification of mycotoxins by enzymatic treatment is compatible with current food technologies.
Examples of enzymatic activities suitable for the detoxification of mycotoxins in food processing
Because fumonisins cause severe, species-specific diseases in farm animals (Voss et al. 2007) and are presumably also in humans (Isaacson 2005), extensive research on fumonisin detoxification has been carried out (Alberts et al. 2016). Enzymes detoxifying fumonisins were found in black yeast Exophiala spinifera (Blackwell et al. 1999) and genetically engineered maize varieties detoxifying fumonisins by enzymes of the yeast were developed (Duvick 2001). Fumonisin-detoxifying bacterium Sphingomonas spp. was characterized (Heinl et al. 2010) to provide enzymes for the decontamination in animal feeds; applications of these enzymes in food production are explicitly considered in the pertaining patent. Fumonisins are polyketides possessing an amino group and esterified with two carballylic acid residues. Microbial degradation of fumonisins is initiated by the hydrolysis of the ester bonds, which reduced the toxicity of fumonisins in pigs (Grenier et al. 2012) but not in rats (Seiferlein et al. 2007). Biochemical studies showed that hydrolyzed fumonisins might be transformed to highly toxic derivatives in vivo (Humpf et al. 1998), which might account for species differences in the toxicity of hydrolyzed fumonisins.
It was shown already in 1988 that the fungus Clonostachys rosea (syn. Gliocladium roseum) is capable to metabolize ZEN in high yield (el-Sharkawy and Abul-Hajj 1988) to a less estrogenic product. Two groups identified the C. rosea gene encoding a ZEN-specific hydrolase (Takahashi-Ando et al. 2002; Karlovsky et al. 2003). The enzyme does not require cofactors and appears suitable for food processing. Further activities detoxifying ZEN have been found in yeasts, bacteria, and fungi (Vekiru et al. 2010; Tan et al. 2014; Popiel et al. 2014).
Numerous enzymes degrading OTA have been described (Abrunhosa et al. 2010). Because OTA is an amide, many peptidases are able to hydrolyze this mycotoxin, including carboxypeptidase and chymotrypsin (Pitout 1969). Numerous lipases were shown to hydrolyze OTA, too (Stander et al. 2000). An enzyme from A. niger cleaves OTA into less toxic OTα and phenylalanine (Dobritzsch et al. 2014).
Enzymatic detoxification appears conceivable for any mycotoxin but a proof of concept for food processing is yet to be provided.
Detoxification of mycotoxins as a side effect of fermentation
Fermentation is food processing with the help of microorganisms. Activities of bacteria and fungi used in fermentations are responsible for desired transformations of food components but hundreds of additional enzymatic activities are present in their cells, actively secreted into food matrix or released from disintegrated cells after autolysis. Some of these activities may transform mycotoxins into non-toxic products (Wolf-Hall and Schwarz 2002) but no microbial strain has been authorized so far as a processing aid targeting mycotoxins. Malting and brewing are prominent examples of technologies that may benefit from such fermentation aids. Considering how much beer per capita is consumed in industrial countries, beer contributes significantly to exposure of consumers to DON and ZEN (see section “Ethanol and beer”). Detoxification of several mycotoxins during malting has been documented, including complete loss of OTA and CIT (see section “Ethanol and beer”) and loss of most of EAs during malting and brewing (Schwarz et al. 2007) but DON survives the process (Scott 1996).
Manufacturing of many dairy products involves fermentation with lactic acid bacteria. The major mycotoxin of concern in milk is aflatoxin M1 (see section “Milk and other dairy products”). Detoxification of aflatoxins by lactic acid bacteria has been studied for three decades (Megalla and Mohran 1984). Unfortunately, we still do not know whether the loss of aflatoxins observed after incubation with lactic bacteria is caused by adsorption, as shown for some lactobacteria (Pierides et al. 2000; Haskard et al. 2001), or whether irreversible enzymatic transformations occurred. This question has not been rigorously addressed so far in spite of its eminent relevance for food safety.
Cultures of yeast Saccharomyces cerevisiae are used in wine making, brewery, and sour dough production. S. cerevisiae was shown to detoxify the mycotoxins PAT (Moss and Long 2002) and OTA (Petruzzi et al. 2014). Alcoholic fermentation of fruit juices destroys PAT, therefore fermented products such as cider and perry will not contain PAT (FAO/WHO 2003). Products of the transformation of ZEN by S. cerevisiae retain their estrogenicity (Matsuura and Yoshizawa 1985; Böswald et al. 1995). Other mycotoxins such as fumonisins and some trichothecenes are not affected by fermentation (Bothast et al. 1992; Schwartz et al. 1995).
An example of technology with potential to reduce mycotoxin content by fermentation is the production of tempeh in Indonesia. Tempeh is traditionally made of soybean fermented with Rhizopus oligosporus. Some current forms of tempeh contain maize and groundnuts, which are prone to contamination with aflatoxins. Interestingly, Rhizopus spp. strains were reported to detoxify aflatoxins (Nakazato et al. 1990). The safety of tempeh can possibly be improved by selecting starter cultures that detoxify aflatoxins without any modification of the technology.
Detoxification of mycotoxins by pure microbial cultures with potential for applications in food production
Except for Rhizopus spp., the ability of food-grade microbial strains to detoxify mycotoxins is limited. Most toxicologically relevant mycotoxins are not detoxified by microbial species used in fermentation. Active strains have to be isolated from other sources. The first screening for mycotoxin-degrading microbes was carried out in the 1960s in the US Department of Agriculture, targeting aflatoxins (Ciegler et al. 1966). Many promising activities were reported but the results were rarely confirmed and advanced. Physical adsorption and enzymatic degradation were seldom differentiated. The unsatisfactory state of knowledge is reminiscent of the removal of aflatoxins by lactic acid bacteria. Numerous further bacterial and fungal species were identified in screening for the detoxification of aflatoxins but progress in elucidating mechanisms of their action was slow. It took 34 years to obtain indication that detoxification of aflatoxins by Flavobacterium aurantiacum is enzymatic (Smiley and Draughon 2000) yet the mechanism is still not known. New microorganisms removing mycotoxins from culture supernatant are continuously being described but follow-up studies are often missing. Degradation of AFB1 by Rhodococcus erythropolis, reported by Teniola et al. (2005) and Alberts et al. (2006) 10 years ago, is a typical example. It is to be hoped that the recent discovery of aflatoxin detoxification by Rhizopus oryzae and Trichoderma reesei (Hackbart et al. 2014) will not share this fate.
In some systems, studies of detoxification of mycotoxins at a molecular level revealed that the activities are not suitable for food processing. This applies in particular to the degradation of AFB1 by Actinomycetes sp. (Ciegler et al. 1966, Hormisch et al. 2004, Teniola et al. 2005, Alberts et al. 2006). The enzymes responsible for aflatoxin degradation in these bacteria are reductases depending on cofactor F 420, which does not occur in microorganisms used in food processing (Taylor et al. 2010). Anaerobic de-epoxidation of trichothecenes by ruminal and intestinal microflora is another example. The activity is known since 1983 (Yoshizawa et al. 1983), the first active pure culture was obtained in 1997 (Binder et al. 1997) but the mechanism and enzyme(s) involved remain unknown.
Yeast used to protect fruits from fungal spoilage may enzymatically destroy the mycotoxin PAT, produced by Penicillium species in infected fruits. Rhodosporidium paludigenum, which is a yeast species studied for fruit protection, degrades PAT into less toxic desoxypatulinic acid (Zhu et al. 2015a). However, treatment of infected apples and pears with R. paludigenum increased the PAT content of the fruits, probably by triggering a stress response in PAT producers (Zhu et al. 2015b). Caution is therefore advised when biological control agents are applied to food commodities. Another example of potentially undesirable side effects of biological control provides ZEN. Because the mycotoxin protects its producer against mycoparasites and competitors (Utermark and Karlovsky 2007), applications of biological control agents to grain contaminated with ZEN-producing Fusarium species might induce increased ZEN production.
Laccases and peroxidases can degrade diverse organic compounds. Degradation products of aflatoxins by peroxidase from white-rot fungus Phanerochaete sordida were elucidated (Wang et al. 2011). In most cases, however, the mechanisms of detoxification remained unknown. For instance, Alberts et al. (2009) demonstrated degradation of AFB1 by laccases of several fungal species and showed that the products were not mutagenic but they have not determined their structures.
The list of microorganisms reported to detoxify mycotoxins is long; we refer to reviews on microbial degradation of mycotoxins in general (Karlovsky 1999; McCormick 2013; Hathout and Aly 2014) and on trichothecenes (He et al. 2010; Karlovsky 2011). New detoxification mechanisms for DON have been discovered recently (Ikunaga et al. 2011; Ito et al. 2013; He et al. 2015, 2016). Although the enzymes involved are still unknown, the use of these strains for the decontamination of food commodities appears promising. As holds for all microorganisms used in food processing, microbial strains for mycotoxin mitigation in food would require regulatory approval.
In this section, examples of raw materials and commodities are given that suffer from high mycotoxin contamination and for which mitigation strategies were studied.
Cereals and derived products
Cereals are staple food worldwide. They are the primary source of carbohydrates and the main source of foodborne mycotoxin exposure. The degree of fungal penetration into the endosperm of grains is reflected in the redistribution of mycotoxins in milling fractions (see section “Physical processing methods”). Due to high mycotoxin concentrations in surface tissues of grains afflicted with Fusarium head Blight, sorting, cleaning, dehulling, and debranning reduce mycotoxin contamination of the flour. A large fraction of mycotoxins can be removed with damaged kernels, fine material, and dust (Cheli et al. 2013). The efficiency of such cleaning in mycotoxin reduction was demonstrated for T-2 and HT-2 toxins (Schwake-Anduschus et al. 2010), fumonisins (Saunders et al. 2001), and DON (Lancova et al. 2008a). Scudamore and Patel (2000) reported effect of cleaning on the content of aflatoxins and fumonisins. In the last decade, several authors reported DON reduction through debranning (e.g., Aureli and D’Egidio 2007). The concept was extended to masked mycotoxins such as DON-3-glucoside (Kostelanska et al. 2011). Recently, the fate of fumonisins along the entire corn meal production chain has been elucidated (Generotti et al. 2015b). Fumonisin content decreased by 40 % in cornmeal semolina; the reduction was less pronounced in corn flour and middlings.
Aflatoxins in cereal matrix can be reduced by soaking (with or without organic acids), cooking/heating, or steaming (Samarajeewa et al. 1990). Ordinary cooking of rice contaminated with AFB1 resulted in a reduction of 34 %; more than 70 % were destroyed by pressure cooking (Park and Kim 2006). The reduction of aflatoxin content by extrusion depends on the presence of additives, moisture level and the applied temperature/pressure; an efficiency of 50 to 80 % can be achieved. Similar effects were reported for OTA in bakery products (Scudamore et al. 2004).
Nixtamalization, which hydrolyzes ester bonds of fumonisins, reduced FB1 content of tortillas by 82 % (Dombrink-Kurtzman et al. 2000). Mycotoxins were retrieved mainly in the steeping and washing water.
Gamma and electron-beam irradiation was evaluated for the reduction of trichothecenes in grains (O’Neill et al. 1993). DON and 3-acetyl-DON were more efficiently destroyed in aqueous solution than on maize kernels.
The effect of bread baking on DON is controversial. While reduction by more than 50 % was observed in one study (Voss and Snook 2010), no changes in DON concentrations during baking was reported by others (Lancova et al. 2008a). The effect of the fermentation of dough on mycotoxins was also evaluated by Vidal et al. (2014). OTA remained stable, whereas DON concentration increased from unkneaded mix to fermented dough and decreased during baking. Zachariasova et al. (2012) observed the opposite: DON concentration decreased from flour to fermented dough and increased during baking. Other authors (Bergamini et al. 2010; Suman et al. 2012, 2014; Generotti et al. 2015a) confirmed the reduction of DON content during baking with the help of a Design of Experiments approach in a pilot plant and industrial production for bread, rusks, and crackers. Baking time and temperature were the key factors of DON reduction. Protease and xylanase used in the bakery industry released additional DON from the matrix during kneading and fermentation of dough (Simsek et al. 2012).
ZEN was reported to remain stable during bread baking (Cano-Sancho et al. 2013) but another study estimated ca. 40 % loss in bread and 20 % in biscuits (Alldrick and Hajšelová 2004). Numanoglu et al. (2013) constructed a kinetic model of ZEN degradation in maize bread during baking.
Nivalenol degradation accelerated with increasing bakery processing temperature (Bretz et al. 2005). Degradation of T-2 and HT-2 toxins by 20–30 % during bread baking was reported (Monaci et al. 2011) but no degradation was observed in another study (Schwake-Anduschus et al. 2010).
As shown earlier, studies of the fate of DON, ZEN, and T-2 during baking within the last 30 years left contradictory conclusions. Results on fumonisins are less extensive but equally contradictory. The first study, conducted more than 20 years ago, reported high losses of fumonisins in heated corn (Scott and Lawrence 1994). A more recent study (Numanoglu et al. 2010) found no significant reduction in the content of DON, ZEN, and fumonisins in traditionally produced Turkish maize bread. Finally, (Bryła et al. 2014) demonstrated reduction of fumonisins by 30 % during baking of gluten-free bread.
Studies of the fate of the depsipeptide mycotoxins enniatins (ENNs) and beauvericin (BEA) during processing of cereals are limited. Vaclavikova et al. (2013) showed that ENNs levels dropped during bread baking to 30 %. Meca et al. (2012) observed BEA degradation from 20 to 90 % in crispy breads during heat treatment and fermentation.
Ergot alkaloids in cereals attract increasing attention of food safety authorities (EFSA 2012). Reduction of sclerotia by up to 80 % can be achieved by winnowing previous to the milling, resulting in substantial reduction of ergot alkaloid content (Berg et al. 1995). Recent incidents of contamination with ergot alkaloids above tolerable levels were reported particularly in small (organic) enterprises (Masloff 2006). An explanation is that equipment for optical sorting is too expensive for small enterprises (Dusemund et al. 2006). Processing sclerotia-contaminated flour in bread, pancakes, or noodles result in a loss of the toxicologically relevant (R)-isomers. Complete decomposition of (R)-isomers of six predominant ergot alkaloids was observed during baking of whole wheat bread, whereas rye bread and triticale pancakes retained 85 and 74 % of these compounds, respectively (Scott and Lawrence 1982). A recent work on biscuits reported degradation and epimerization of up to 30 % ergot alkaloids to less toxic forms (Merkel et al. 2012).
In pasta, the solubility of mycotoxins in boiling water determines the level of consumer exposure. According to Visconti et al. (2004), most DON was extracted from pasta to cooking water. Brera et al. (2013) observed DON reduction in semolina in dry and cooked pasta by 8 and 41 %, respectively.
Cocoa is grown in West Africa, Asia, and Latin America mainly as raw material for chocolate production. Suboptimal storage and processing conditions in producing areas frequently cause fungal contaminations producing aflatoxins and OTA (Copetti et al. 2014). In order to minimize the OTA contamination of cocoa, cocoa-producing countries are developing new post-harvest treatment guidelines. OTA is concentrated in beans shells and therefore toxin levels in the nibs are low: mechanical shelling removed 48 % of OTA (Gilmour and Lindblom 2008), while shelling by hand reduced OTA to between 50 and 100 % (Amezqueta et al. 2005).
The first step of cocoa processing is opening the harvested pods at the farm site; then, the beans are fermented naturally by yeasts and bacteria. Experiments in Brazil (Copetti et al. 2012) demonstrated the importance of organic acids produced by fermentative bacteria in suppressing the growth of fungi with the potential to produce OTA. Fermented beans are dried in the sun on wooden platforms or on the ground. The OTA level significantly increases during transition from fermentation to drying (Dano et al. 2013). Drying must be therefore conducted as rapidly as possible. In processing plants, dried beans are broken and winnowed to obtain de-shelled kernels (nibs). The nib is sterilized with steam and roasted directly without (natural) or with addition of alkali to develop the final flavor and color. The temperature reaches 100 to 120 °C for a period of 15 to 70 min; it is not expected that OTA is degraded significantly in this step (Mounjouenpou et al. 2012). However, degradation of OTA by 17 to 40 % was reported in different experiments (Manda et al. 2009; Copetti et al. 2013).
The effect of roasting on aflatoxins in cocoa was evaluated by Mendez-Albores et al. (2013); roasting cocoa beans at 250 °C for 15 min reduced aflatoxin content by up to 71 %. Mycotoxin levels in alkalized cocoa powder tend to be reduced (Copetti et al. 2011; Turcotte et al. 2013). Alkalization appeared to be more effective in reducing aflatoxin than OTA. Results of Mendez-Albores et al. (2013) showed reduction in aflatoxin content in cocoa liquors due to the thermal-alkaline treatment up to 98 %. Turcotte et al. (2013) reported that OTA and AFB1 occurred ubiquitously in natural and alkalized cocoa, decreasing progressively from cocoa liquor to baking chocolate, to dark chocolate, and milk chocolate; no OTA was found in cocoa butter. Overall, it can be inferred that processing cocoa bean to chocolate leaves negligible concentrations of OTA and aflatoxins in the final product. In addition to the degradation described earlier, production of chocolate includes addition of other ingredients (e.g., milk products and sugars) which further dilutes mycotoxins in the final commodity.
The European Commission currently considers that introducing maximum limit of OTA in cocoa or cocoa products does not appear necessary for the protection of public health (European Commission (EC) Regulation No. 1881/2006). Indeed, samples of cocoa containing more than 2 ng/g of these mycotoxins can rarely be found.
Green coffee beans are one of numerous food commodities significantly contaminated with OTA (Speijers and van Egmond 1993). Coffee and cocoa beans are hygroscropic and thus vulnerable to contamination with OTA during storage and transport (Magan and Aldred 2005). Scudamore (2005) reviewed the effect of roasting and brewing of coffee on the level of OTA as compared to green coffee beans. OTA levels were drastically reduced during production of soluble coffee. The roast and the ground of coffee contained only 16 % of the concentration found in raw green coffee beans (Blanc et al. 1998). Similarly, Milanez (1996) reported 84 % of OTA reduction in processed beans. However, other investigators found lower OTA reduction (Leoni et al. 2000).
The major mycotoxin in fruit juices is PAT. The starting point of reducing PAT in apple-derived products is the selection of intact apples and the removal of rotten apples. Several studies have shown that PAT is stable in slightly acidic apple and grape juices but is decomposed during the production of cider (Moss and Long 2002). Alcoholic fermentation converts PAT into ascladiol, which is less toxic than PAT (Suzuki et al. 1971). A number of studies on the effect of different physical, chemical, or microbiological food processes on PAT concentrations have been performed (Leggett et al. 2001; Castoria et al. 2011; Zhu et al. 2015b). PAT reacts with sulfhydryl (thiol) groups of proteins, polypeptides, and amino acids available in certain food commodities such as cereals to form intra- and intermolecular protein cross links. These PAT adducts are not detected by conventional methods for PAT analysis (Fliege and Metzler 1999).
Milk and other dairy products
AFB1 contaminating dairy feed may be metabolized in the animal into its monohydroxy derivative form aflatoxin M1 which is carried over into milk (Holzapfel et al. 1966). AFM1 or its metabolites can then contaminate subsequent dairy products. Distribution and stability of AFM1 during processing, ripening, and storage of Telemes cheese was studied by Govaris et al. (2001). Concentration of AFM1 in the curds was about four times higher than in milk but it fell in the cheese during ripening. In certain cheese kinds in Turkey, the concentration of AFM1 was, however, higher in the cheese than in bulk milk (Bakiri 2001). Fermentation of milk to yogurt at pH 4.6 and 4.0 reduced AFM1 concentration by 13 and 22 %, respectively; total loss of AFM1 after storage was 16 and 34 %, respectively (Govaris et al. 2002). In opposition with these findings, Yousef and Marth (1989) reported that AFM1 in fermented milk remained stable. Separation of milk components partitioned the toxin in accordance with its affinity for casein and the lack of solubility in fats.
Mahoney and Molyneux (2010) assumed that aflatoxins are not found in vegetable oils but there is increasing evidence that this does not apply to non-purified or crude vegetable oils (Shephard et al. 2011). High incidences of aflatoxin contaminations in edible vegetable oils were even reported (Bordin et al. 2014). The different processes used for vegetable oil extraction may partially explain these discrepancies. Edible vegetable oils indeed can be extracted from oleaginous material either by mechanical pressing or by solvents.
Distribution of mycotoxins from steeped corn to corn germs in wet milling depends on their solubility. Water-soluble mycotoxins such as DON were found at high concentrations in steep liquor but at low levels in the solid (germ, fiber, and gluten) fractions. The inverse is true for ZEN, which is relatively insoluble in water (Table 2). ZEN may occur in maize germ oil but investigations about the effect of processing of oils on ZEN are limited, except for UV light exposure during storage (see section “Irradiation”). Abalaka and Elegbede (1982) reported that only 10–20 % of aflatoxins were transferred from groundnuts and cotton seeds to crude oil. Similarly, Parker and Melnick (1966) found most aflatoxins after extraction of oil from peanut and maize in defatted meal. In their work, corn oil extracted with chloroform contained more aflatoxins than oil extracted with hexane. Refining based on alkaline neutralization, washing, bleaching, and deodorization was reported to remove mycotoxins from oil (Kamimura et al. 1986). There are no studies in the literature relating degumming to the presence of aflatoxins.
In the deacidification step of refining, fatty acids are removed using an alkali (chemical refining) or water vapor (physical refining). Alkaline treatment is effective in degrading aflatoxins (see section “Treatment with bases”). Kamimura et al. (1986) evaluated the efficiency of the processing steps of refining vegetable oils contaminated with mycotoxins and observed that after treatment with sodium hydroxide, AFB1 and AFB2 were significantly reduced, DON and NIV were present only in traces and AFG1 and AFG2 were completely eliminated. These results confirmed an earlier finding by Parker and Melnick (1966) that sodium hydroxide efficiently removed aflatoxins from peanut oil. ZEN is removed below a level of concern from corn germ oil by alkaline treatment at pH 9–10 (Slope et al. 2013).
Bleaching consists of introducing an adsorptive bleaching material, called bleaching earth or clay, under vacuum condition and high temperature, and subsequent removal of the agent by filtration. Parker and Melnick (1966) found that bleaching reduced aflatoxin levels in peanut and corn oils below 1 μg/L. Kamimura et al. (1986) reported that bleaching of vegetable oil eliminated trichothecenes and aflatoxins but not ZEN.
The last step of the refining process of vegetable oil is the deodorization step, which is a codistillation process using water vapor under high temperature (220–270 °C) and low pressure (0.1–0.7 kPa). Conditions adopted in the deodorizing process can lead to complete removal of aflatoxins from vegetable oils, as Kamimura et al. (1986) showed for artificially contaminated vegetable oils. The levels of trichothecene and ZEN were reduced, too. Thus, it is possible to ensure safe edible vegetable oil provided it is properly processed.
Ethanol and beer
In process where fermentation is followed by distillation, mycotoxins are not present in the alcohol fraction but may be increased in the spent grain product. AFB1 was decreased by 47 % after cooking and fermenting contaminated corn or wheat (Dam et al. 1977), although its concentration in the solids after distillation was higher than in the starting grain. No ZEN from contaminated corn appeared in ethanol but ZEN concentration in the solids after fermentation doubled. Two lots of corn contaminated with 15 and 36 mg/kg of FB1 were fermented for ethanol production (Bothast et al. 1992). Analysis of the various fermentation products showed that there was little fumonisin degradation during fermentation. No FB1 was found in distilled ethanol or centrifuged solids. Most of the fumonisins were distributed over distillers’ dried grains, thin stillage, and distillers’ solubles.
Most mycotoxins can survive brewing and end up in the beer; DON is found at highest concentrations (Scott 1996). Lancova et al. (2008b), however, reported that even larger amounts of DON in beer were present in the form of DON-3-glucoside. Schwartz et al. (1995) found that in the brewing process both ZEN and 15-acetyl-DON increased during germination of barley. Eighty to 93 % of DON from malt grist was found in the beer; 60 % of ZEN and 18 % of 15-acetyl-DON were found in the spent grains. Scott and Lawrence (1995) examined the losses of OTA as well as of FB1 and FB2 in fermentation of wort. Up to 21 % of OTA but negligible amounts of fumonisins were taken up by the yeast, indicating that OTA and fumonisins could contaminate beer. Fumonisins were later indeed detected in domestic and imported beer in Canada (Scott et al. 1993). Most recently, the fate of 14 mycotoxins during beer brewing was investigated by Inoue et al. (2013). After brewing, the levels of aflatoxins, OTA, FB2, PAT, and ZEN dropped below 20 %. ZEN and PAT were apparently metabolized to less toxic compounds. Trichothecenes survived brewing at more than 50 % of their initial concentration. Vaclavikova et al. (2013) reported that enniatins from contaminated wheat and barley were not transferred into beer; these hydrophobic mycotoxins remained adsorbed on spent grain.
Dried fruits, nuts, and spices
Food safety is significantly affected by mycotoxin contamination of dried fruits, nuts, and spices. Dry fruits such as raisins, sultanas, figs, apricots, and dates are consumed worldwide. Cultivation and processing of these fruits in warm climates rise mycotoxin risk, especially concerning aflatoxins and ochratoxin A. The pH of fruits ranging from 2.5 to 5.0 is the most important factor affecting spoilage of fruits by microorganisms. Fruits become increasingly susceptible to fungal invasion during ripening, as the pH of the tissue increases and skin layers soften (Drusch and Ragab 2003). Other factors contamination level are harvesting and drying conditions and moisture content (Bullerman et al. 1984). Physical cleaning and separation, where the mold-damaged kernel/seed/nuts are removed, can result in 40–80 % reduction in aflatoxin level. Dry and wet milling may redistribute aflatoxins into less utilized fractions.
In groundnuts, higher levels of aflatoxin are associated with small, immature pods. Removing these pods reduces aflatoxin concentration in shelled lots (Dorner 2008). To remove foreign material and unshelled pods, shelled peanuts are subjected to gravity separation. Because highly contaminated kernels are less dense, this process reduces aflatoxin contamination (Davidson et al. 1981). Shelled kernels can be further separated by size through a series of slotted screens; generally, aflatoxins are associated with smaller-sized kernels (Whitaker et al. 2005). Further, aflatoxin reduction can be obtained by blanching combined with electronic color sorting (Cole et al. 1995) and sorting with the help of IR and UV spectroscopy (Durmus and Kalkan 2016). Blanching and color sorting is used for other nuts such as pistachios, too.
Among dried fruits, figs are the most challenging. Removal of damaged fruits, solar drying, fluorescence sorting, and treatment with sulfur dioxide are effective mycotoxin mitigation strategies (Scott and Trucksess 2009). Sun-drying figs are often practiced in tropical countries but because it is slow, it allows proliferation of molds producing mycotoxins. Ozay et al. (1995) showed that dipping figs in solution of metabisulfite or sorbate or in hot water followed by dehydration reduced fungal colonization and aflatoxin content. Comparison of different drying systems with sun-drying revealed that ultrasound treatment combined with osmotic solutions is most effective (Villalobos et al. 2016).
Very recently, the use of cold atmospheric plasma to destroy aflatoxins in dehulled hazelnuts was reported, with over 70 % aflatoxin reduction achieved by 12 min of treatment (Siciliano et al. 2016). Another recent work demonstrated that roasting pistachio nuts with lemon juice and/or citric acid destroyed over 90 % AFB1 (Rastegar et al. 2016).
To the best of our knowledge, the effect of food processing on mycotoxin levels in spices was not studied. A moderate reduction of OTA and aflatoxin content in pepper by gamma radiation was reported (Jalili et al. 2012).
Implications and outlook
Mycotoxin contamination of food commodities, especially of staple foods, poses a serious threat to human health. Efficient reduction of mycotoxin exposure via food products requires the utilization of all available technologies from good agricultural and storages practices and selection of raw materials suitable for human consumption to the application of food processing technologies including biotechnology. Today’s consumers are keenly aware of the importance of food for their health. Their perception of food safety has been heavily biased towards man-made pollutants but toxic compounds of natural origin are slowly gaining attention. Food industry has recognized the trend, as intellectual property protection efforts in mycotoxin detoxification show (He and Zhou 2010).
Mitigation of mycotoxins as a side effect of established food processing techniques, such as fermentation of apple juice or nixtamalization of maize flour, should be utilized whenever possible. Development of new techniques dedicated to mycotoxin mitigation will, however, require extensive research. The impact of mycotoxin mitigation processes on the nutritional composition and organoleptic quality of food and their influence on other contaminants such as acrylamide (Anese et al. 2009) have to be assessed. The advantage of reducing mycotoxin levels has to be weighed against the loss of material and/or nutrients. Novel physical and chemical treatments (cold plasma) and novel detoxification agents (microbes or purified enzymes) for mycotoxin mitigation in food would have to undergo regulatory approval, which implies a risk analysis. European regulation 1881/2006 provides direction in what is likely to be acceptable; similar regulations operate outside Europe (refer also to EC regulation 2015/786).
Disappearance of a parent mycotoxin does not necessarily mean detoxification, if the toxin is converted into a form that escapes detection, yet remains toxic. Often, the mechanism of mycotoxin transformation is not fully understood, the products have not been characterized, and their bioavailability and toxicity compared to the parent compound has not been assessed. Limited toxicological investigations on mycotoxin degradation products were restricted to in vitro and acute in vivo studies which provide insufficient information regarding the safety at chronic low level exposure. New predictive tools in toxicology (Schilter et al. 2014; Kavlock et al. 2012; Cozzini and Dellafiora 2012) may be helpful in identifying transformation or degradation products requiring detailed toxicological investigation. Without such knowledge and as a precautionary approach, risk assessment has to assume that all mycotoxin forms have the same bioavailability and toxicity as the respective parent compound (EFSA 2014).
While detoxification of mycotoxins has been studied extensively, little is known about the potential of food processing to increase or hide mycotoxin exposure. Chemical and physical treatments applied to food may release mycotoxins from masked forms and make them bioavailable or convert mycotoxins into forms not detectable by conventional analytical methods (Rychlik et al. 2014) while retaining their toxic potential (Suman and Generotti 2015) or stimulate contaminating fungi to mycotoxin production, e.g., during steeping of barley. Analytical tools for mycotoxins transformed by processing (structural modification, binding to food matrix) need to be developed.
Most research on mycotoxins focused on regulated mycotoxins. The recent unexpected discovery of the fungus Stachybotrys chartarum in culinary herbs (Biermaier et al. 2015) showed that the scope has to be broadened: S. chartarum, so far known mainly from water-damaged walls, produces macrocyclic trichothecenes of higher acute toxicity than any regulated mycotoxin. Highly toxic metabolites of the fungus Stenocarpella maydis recently discovered in maize grains provide another example of toxicologically relevant non-regulated mycotoxins in food (Rogers et al. 2014). Even well-studied fungal metabolites may raise new food safety concerns, such as AAL toxin of Alternaria alternata if it occurs in tomato juice (Karlovsky 2016). Genome sequencing revealed that fungi-contaminating food commodities have the potential to produce 30–60 secondary metabolites each, some of which might turn out to be mycotoxins. Mitigation strategies have to be adapted to newly discovered mycotoxins, once their toxicity and level of exposure have been assessed. Undoubtedly, the list of regulated mycotoxins will grow.
The large number of combinations of processing/commodities/mycotoxins calls for prioritization among applications on which to focus further research efforts. Criteria for prioritization should be consumption of the contaminated commodity (staple foods and commodities consumed by sensitive population groups like young children), occurrence at high levels in such commodities and unfavorable toxicological profiles. Different geographical regions and target groups require different prioritizations. An example is fumonisin contamination of maize: in Africa, maize is a staple food, causing human exposure to fumonisins that would exceed the tolerable daily intake (TDI) even if EU limits of contamination could be achieved (Shephard et al. 2007; JECFA 2012). Celiac disease patients in industrialized countries consume above-average amounts of maize; it was shown that their dietary exposure to fumonisins clearly exceeds average levels (Dall’Asta et al. 2012). Most consumers in Europe, however, would not exceed TDI for fumonisins even if they consume maize exceeding the EU limit by an order of magnitude. Guidelines have been elaborated on how to identify relevant targets for mitigation, determine the effectiveness of mitigation measures, and assess the risk of unintended consequences (van der Fels-Klerx et al. 2014).
Food processing can reduce mycotoxin exposure by destroying or eliminating mycotoxins, by transforming them into less toxic derivatives, by adsorbing mycotoxins to solid surfaces or by reducing their bioavailability due to chemical attachment to food matrix structures. Complete elimination of mycotoxins from food product by processing can rarely be achieved.
Several processing techniques of proven value (mostly physical treatments) have been in use for a long time. These are the only mycotoxin mitigation methods currently applicable to human food. Few chemical and biotechnological techniques reducing mycotoxin content have been approved for animal feed but many promising strategies remain at an experimental stage. In addition to mycotoxin derivatives modified by microorganisms or plants (EFSA 2014), the risk assessment of mycotoxins in food has to include mycotoxin forms resulting from food processing. Before a novel mycotoxin decontamination technique is approved, chemical identity and toxicity of the reaction products have to be determined. Availability of analytical methods which permit reliable detection of these products is a key prerequisite for risk assessment. Bioavailability and toxicity of transformation products should be assessed using a systematic approach and generally acknowledged testing systems. This in turn allows prioritizing those which require more detailed toxicological assessment and the choice of specific, adequate risk assessment options. Recently established legislative criteria for detoxification processes applied to feed may serve as a model (EC 2015). In the absence of adequate toxicological data, mycotoxin forms generated during processing must be assumed to have the same toxicity, bioavailability and carcinogenic potency as the respective parent compounds. The development of mitigation strategies should prioritize mycotoxins that regularly occur at high levels in highly consumed commodities and have unfavorable toxicological profiles. The ultimate goal of mycotoxin mitigation is to prevent adverse health effects caused by foodborne exposure to mycotoxins while preserving nutritional and organoleptic quality of food.
Source of funding
This work was commissioned by the Process-related Compounds and Natural Toxins Task Force of the European branch of the International Life Sciences Institute (ILSI Europe). Industry members of this task force (2013–2016) are Abbott Nutrition, ADM, Barilla G&R Fratelli, Bunge Europe, Campbell, Cargill, Kao Corporation, Kellog Europe, Kikkoman Foods Europe, Luigi Lavazza, Mondelez International, Mars, Nestlé, PepsiCo International, Premier Foods, Südzucker/BENEO Group, Unilever. The opinions expressed herein and the conclusions of this publication are those of the authors and do not necessarily represent the views of ILSI Europe nor those of its member companies.
Conflict of interest
Academic members of this expert group received support from ILSI Europe the Process-related Compounds and Natural Toxins Task Force, consisting of reimbursement of travel costs and a small honorarium.
- Afriyie-Gyawu E, Ankrah NA, Huebner HJ, Ofosuhene M, Kumi J, Johnson NM, Tang L, Xu L, Jolly PE, Ellis W, Ofori-Adjei D, Williams JH, Wang JS, Phillips TD (2008) NovaSil clay intervention in Ghanaians at high risk for aflatoxicosis. I. Study design and clinical outcomes. Food Addit Contam 25:76–87CrossRefGoogle Scholar
- Altug T, Youssef AE, Marth EH (1990) Degradation of aflatoxin B1 in dried figs by sodium bisulfite with or without heat, ultraviolet energy or hydrogen peroxide. J Food Prot 53:581–628Google Scholar
- Applebaum RS, Marth HE (1982) Inactivation of aflatoxin M1 in milk using hydrogen peroxide and hydrogen peroxide plus riboflavin or lactoperoxidase. J Food Prot 45:557–560Google Scholar
- Aureli G, D’Egidio MG (2007) Efficacia della decorticazione nella riduzione del DON in frumento duro. Tecnica Molitoria 58:729–733Google Scholar
- Bennett GA, Peplinski AJ, Brekke OL, Jackson LK, Wichser WR (1976) Zearalenone—distribution in dry-milled fractions of contaminated corn. Cereal Chem 53:299–307Google Scholar
- Berg T, Rasmussen G, Thorup I (1995) Mycotoxins in Danish foods. National Food Agency of Health, SoborgGoogle Scholar
- Bergamini E, Catellani D, Dall’Asta C, Galaverna G, Dossena A, Marchelli R, Suman M (2010) Fate of Fusarium mycotoxins in the cereal product supply chain: the deoxynivalenol (DON) case within industrial bread-making technology. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 27:677–687PubMedCrossRefGoogle Scholar
- Bfr, Bundesamt für Risikobewertung (2004) Mutterkornalkaloide in Roggenmehl. Stellungnahme des BfR vom 22. Januar 2004, http://www.bfr.bund.de/cm/343/mutterkornalkaloide_in_roggenmehl.pdf. Accessed 16 July 2016
- Binder J, Horvath EM, Schatzmayr G, Ellend N, Danner H, Krska R, Braun R (1997) Screening for deoxynivalenol-detoxifying anaerobic rumen microorganisms. Cereal Res Commun 25:343–346Google Scholar
- Bordin K, Sawada MM, Rodrigues CEC, da Fonseca CR, Oliveira CAF (2014) Incidence of aflatoxins in oil seeds and possible transfer to oil: a review. Food Eng Rev 6:20–28Google Scholar
- Bullerman LB, Schroeder LL, Park KY (1984) Formation and control of mycotoxins in food. J Food Prot 47:637–646Google Scholar
- Castegnaro M, Friesa M, Michelon J, Walker EA (1981) Problems related to the use of sodium hypochlorite in the detoxification of aflatoxin B1. J Occup Environ Hygiene 42:398–401Google Scholar
- Castoria R, Mannina L, Durán-Patrón R, Maffei F, Sobolev AP, De Felice DV, Pinedo-Rivilla C, Ritieni A, Ferracane R, Wright SAI (2011) Conversion of the mycotoxin patulin to the less toxic desoxypatulinic acid by the biocontrol yeast Rhodosporidium kratochvilovae strain LS11. J Agric Food Chem 59:11571–11578PubMedCrossRefGoogle Scholar
- Chakrabarti AG (1981) Detoxification of corn. J Food Prot 44:591–592Google Scholar
- Cheftel JC (1989) Extrusion cooking and food safety. In: Mercier C, Linko P, Harper JM (eds) Extrusion cooking. American Association of Cereal Chemists, Minnesota, pp. 435–461Google Scholar
- Choudhari AK, Kumari P (2010) Management of mycotoxin contamination in preharvest and postharvest crops: present status and future prospects. J Phytology 2:37–52Google Scholar
- Codex Alimentarius (2015) General Standard for Food Additives (GSFA) Online Database. http://www.fao.org/fao-who-codexalimentarius/download/standards/4/CXS_192_2015e.pdf. Accessed 16 July 2016
- Cole RJ, Dorner JW, Holbrook CC (1995) Separation and removal of aflatoxin contaminated kernels in peanut shelling plants. In: Stalker HT, Pattee HE (eds) Advances in peanut science, 1st edn. American Peanut Research and Education Society, Cornell, pp. 456–474Google Scholar
- Conway HF, Anderson RA, Bagley EB (1978) Detoxification of aflatoxin-contaminated corn by roasting. Cereal Chem 55:115–117Google Scholar
- Dam R, Tam SW, Satterlee LD (1977) Destruction of aflatoxins during fermentation and by-product isolation from artificially contaminated grain. Cereal Chem 54:705–714Google Scholar
- Dänicke S, Valenta H, Gareis M, Lucht HW, von Reichenbach H (2005) On the effects of a hydrothermal treatment of deoxynivalenol (DON)-contaminated wheat in the presence of sodium metabisulphite (Na2S2O5) on DON reduction and on piglet performance. Anim Feed Sci Technol 118:93–108CrossRefGoogle Scholar
- Davidson JI Jr, Holaday CE, Bennett CT (1981) Separation and removal of aflatoxin contaminated kernels in peanut shelling plants. Proc Am Peanut Res Edu Soc 13:29–45Google Scholar
- de Nijs M, van Egmond HP, Nauta M, Rombouts FM, Notermans S (2012) Mycotoxin binders in feed: food safety aspects. In report 2011.019 of RIKILT, Institute of Food Safety. Wageningen University (University& Research Centre), WageningenGoogle Scholar
- Doyle MP, Applebaum RS, Brackett RE, Marth EH (1982) Physical, chemical and biological degradation of mycotoxins in foods and agricultural commodities. J Food Prot 45:964–971Google Scholar
- Doyle MP, Marth EH (1978a) Bisulfite degrades aflatoxin: effect of temperature and concentration of bisulfite. J Food Prot 41:774–780Google Scholar
- Doyle MP, Marth EH (1978b) Bisulfite degrades aflatoxin: effect of citric acid and methanol and possible mechanism of degradation. J Food Prot 41:891–896Google Scholar
- Draughon FA, Childs EA (1982) Chemical and biological evaluation of aflatoxin after treatment with sodium hypochlorite, sodium hydroxide and ammonium hydroxide. J Food Prot 45:703–706Google Scholar
- Dusemund B, Altman HJ, Lampen A (2006) II. Toxicologischer Bewertung Mutterkornalkaloid-kontaminierter Roggenmehle. J Consum Prot Food Safety 1:150–152Google Scholar
- EC, European Commission (2006) Regulation no 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Off J Eur U L 364:5Google Scholar
- EC, European Commission (2013) Aflatoxin contamination in hazelnuts and dried figs intended for export to the EU, http://ec.europa.eu/food/fvo/act_getPDF.cfm?PDF_ID=10210
- EC, European Commission (2015) Regulation 2015/786 defining acceptability criteria for detoxification processes applied to products intended for animal feed. Off J Eur Union 125:10–14Google Scholar
- EFSA, European Food Safety Authory (2009) Guidance on the submission of a Dossier on food enzymes for safety evaluation by the scientific panel of food contact material, enzymes, flavourings and processing aids. EFSA J 1305:1–26Google Scholar
- EFSA, European Food Safety Authority Panel on Contaminants in the Food Chain (CONTAM) (2004) EFSA scientific opinion on deoxynivalenol (DON) as undesirable substance in animal feed. EFSA J 73:1–42Google Scholar
- EFSA, European Food Safety Authority Panel on Contaminants in the Food Chain (CONTAM) (2005) EFSA scientific opinion on fumonisins as undesirable substances in animal feed. EFSA J 235:1–32Google Scholar
- EFSA, European Food Safety Authority Panel on Contaminants in the Food Chain (CONTAM) (2006) EFSA scientific opinion on ochratoxin a in food. EFSA J 365:1–56Google Scholar
- EFSA, European Food Safety Authority Panel on Contaminants in the Food Chain (CONTAM) (2007) European food safety authority (EFSA) scientific opinion related to the potential increase of consumer health risk by a possible increase of the existing maximum levels for aflatoxins in almonds, hazelnuts and pistachios and derived products. EFSA J 446:1–127Google Scholar
- FAO/WHO (2003) Codex Alimentarius: CAC/RCP 50—Code of practice for the prevention and reduction of patulin contamination in apple juice and apple juice ingredients in other beverages. Food and Agriculture Organization. http://www.fao.org/input/download/standards/405/CXP_050e.pdf. Accessed 19 May 2016
- Fouler SG, Trivedi AB, Kitabatake N (1994) Detoxification of citrinin and ochratoxin a by hydrogen peroxide. J Assoc Off Agric Chem Int 77:631–637Google Scholar
- Fraenkel H (1962) Colour sorting machine. US Patent No. 3,066,797Google Scholar
- Generotti S, Cirlini M, Malachova A, Sulyok M, Berthiller F, Dall’Asta C, Suman M (2015a) Deoxynivalenol and deoxynivalenol-3-glucoside mitigation through bakery production strategies: effective experimental design within industrial rusk-making technology. Toxins 7:2773–2790PubMedPubMedCentralCrossRefGoogle Scholar
- Gilmour M, Lindblom M (2008) Management of OTA in the cocoa supply chain: a summary of work by the CAOBISCO/ECA/FCC working group on OTA. In: Leslie JF, Bandyopadhyay R, Visconti A (eds) Mycotoxins: detection methods, management, Public Health and Agricultural Trade. CABI, Wallingford, pp. 231–243Google Scholar
- Grenier B, Bracarense AP, Leslie JF, Oswald IP (2014) Physical and chemical methods for mycotoxin decontamination in maize. In: Leslie JF, Logrieco AF (eds) Mycotoxin reduction in grain chains. Wiley Blackwell, New Delhi, pp. 116–129Google Scholar
- Grenier B, Bracarense A-PFL, Schwartz HE, Trumel C, Cossalter A-M, Schatzmayr G, Kolf-Clauw M, Moll W-D, Oswald IP (2012) The low intestinal and hepatic toxicity of hydrolyzed fumonisin B1 correlates with its inability to alter the metabolism of sphingolipids. Biochem Pharmacol 83:1465–1473PubMedCrossRefGoogle Scholar
- Hagler WM, Winston M, Hutchins JE, Hamilton PB (1982) Destruction of Aflatoxin in corn with sodium bisulfite. J Food Prot 45:1287–1291Google Scholar
- Hahn I, Nagl V, Schwartz-Zimmermann HE, Varga E, Schwarz C, Slavik V, Reisinger N, Malachová A, Cirlini M, Generotti S, Dall’Asta C, Krska R, Moll WD, Berthiller F (2015) Effects of orally administered fumonisin B1 (FB1), partially hydrolysed FB1, hydrolysed FB1 and N-(1-deoxy-D-fructos-1-yl) FB1 on the sphingolipid metabolism in rats. Food Chem Toxicol 76:11–18PubMedCrossRefGoogle Scholar
- Hormisch D, Brost I, Kohring GW, Giffhorn E, Kroppenstedt RM, Stackebrandt E, Färber P, Holzapfel WH (2004) Mycobacterium fluoranthenivorans sp nov., a fluoranthene and aflatoxin B-1 degrading bacterium from contaminated soil of a former coal gas plant. Syst Appl Microbiol 27:653–660PubMedCrossRefGoogle Scholar
- Huff WE (1980) A physical method for the segregation of aflatoxin-contaminated corn. Cereal Chem 57:236–238Google Scholar
- Huff WE, Hagler WM (1985) Density segregation of corn and wheat naturally contaminated with aflatoxin, deoxynivalenol and zearalenone. J Food Prot 48:416–420Google Scholar
- Humpf H-U, Schmelz EM, Meredith FI, Vesper H, Vales TR, Wang E, Menaldino DS, Liotta DC, Merrill AH (1998) Acylation of naturally occurring and synthetic 1-deoxysphinganines by ceramide synthase—formation of N-palmitoyl-aminopentol produces a toxic metabolite of hydrolyzed fumonisin, AP(1), and a new category of ceramide synthase inhibitor. J Biol Chem 273:19060–19064PubMedCrossRefGoogle Scholar
- IARC, International Agency for Research on Cancer (1986) Monographs on the evaluation of carcinogenic risk of chemicals to humans. Volume 40: Some naturally occurring and synthetic food components, furocoumarins and ultraviolet radiationGoogle Scholar
- IARC, International Agency for Research on Cancer (1993) Monographs on the evaluation of carcinogenic risks to humans volume 56. Some naturally occurring substances: Food items and constituents, heterocyclic aromatic amines and mycotoxinsGoogle Scholar
- IARC, International Agency for Research on Cancer (2002) Monographs on the evaluation of carcinogenic risks to humans volume 82: Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. International Agency for Research on Cancer.Google Scholar
- IARC, International Agency for Research on Cancer (2012) Monographs on the evaluation of carcinogenic risks to humans volume 100F: chemical agents and related occupationsGoogle Scholar
- Ikunaga Y, Sato I, Grond S, Numaziri N, Yoshida S, Yamaya H, Hiradate S, Hasegawa M, Toshima H, Koitabashi M, Ito M, Karlovsky P, Tsushima S (2011) Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol. Appl Microbiol Biotechnol 89:419–427PubMedCrossRefGoogle Scholar
- Jacobsen BJ (2014) Good agricultural and harvest practices to reduce mycotoxin contamination in wheat in temperate countries. In: Leslie JF, Logrieco AF (eds) Mycotoxin reduction in grain chains. Wiley Blackwell, New Delhi, pp. 209–219Google Scholar
- JECFA, Joint FAO/WHO Expert Committee on Food Additives (1996) Evaluation of certain veterinary drug residues in food, Prepared by the 45th JECFA meeting Additives. WHO Technical Report Series, No. 864Google Scholar
- JECFA, Joint FAO/WHO Expert Committee on Food Additives (1999) Safety evaluation of certain food additives and contaminants, Prepared by the 49th JECFA meeting. WHO Technical Report Series, No. 884Google Scholar
- JECFA, Joint FAO/WHO Expert Committee on Food Additives (2000) Safety evaluation of certain food additives and contaminants, Prepared by the 51st JECFA meeting. WHO Technical Report Series, No. 891Google Scholar
- JECFA, Joint FAO/WHO Expert Committee on Food Additives (2001a) Safety evaluation of certain food additives and contaminants, Prepared by the 55th JECFA meeting. WHO Technical Report Series, No. 901Google Scholar
- JECFA, Joint FAO/WHO Expert Committee on Food Additives (2001b) General specifications and considerations for enzyme preparations. Prepared by the 57th JECFA meetingGoogle Scholar
- JECFA, Joint FAO/WHO Expert Committee on Food Additives (2011) Safety evaluation of certain food additives and contaminants, Prepared by the 74th JECFA meeting, WHO Technical Report Series, No. 966Google Scholar
- JECFA, Joint FAO/WHO Expert Committee on Food Additives (2012) Safety evaluation of certain food additives and contaminants, Prepared by the 76th JECFA meeting, WHO Technical Report Series, No. 974Google Scholar
- Karlovsky P (2014) Enzymatic detoxification of mycotoxins for healthy food. New Food 17:66–69Google Scholar
- Karlovsky P (2016) Detoxification strategies for mycotoxins in plant breeding. In: Dall’Asta C, Berthiller F (eds) Masked mycotoxins in food: formation, occurrence and toxicological relevance. Royal Society of Chemistry, Cambridge, pp. 158–188Google Scholar
- Karlovsky P, Crane E III, Gilliam JT, Maddox JR (2003) Compositions and methods of zearalenone detoxification. US patent No. 20030073239, pp 1–30Google Scholar
- Kavlock R, Chandler K, Houck K, Hunter S, Judson R, Kleinstreuer N, Knudsen T, Martin M, Padilla S, Reif D, Richard A, Rotroff D, Sipes N, Richard A (2012) Update on EPA’s ToxCast program: providing high throughput decision support tools for chemical risk assessment. Chem Res Toxicol 25:1287–1302PubMedCrossRefGoogle Scholar
- Lancova K, Hajslova J, Poustka J, Krplova A, Zachariasova M, Dostalek P, Sachambula L (2008b) Transfer of fusarium mycotoxins and “masked” deoxynivalenol (deoxynivalenol-3-glucoside) from field barley through malt to beer. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 25:732–744PubMedCrossRefGoogle Scholar
- Masri MS, Vix HLE, Goldblatt LA (1969) Process for de-toxifying substances contaminated with aflatoxin. United States Patent No. 3.429.709Google Scholar
- Mayer J (1898) Grain-sorting machine. United States Patent No. 671,500Google Scholar
- Mendez-Albores A, Campos-Agular AZ, Moreno-Martinez E, Vazquez-Duran A (2013) Physical and chemical degradation of B-aflatoxins during the roasting and dutching of cocoa liquor. J Agr Sci Tech 15:557–567Google Scholar
- Mesterhazy A (2014) Breeding for resistance to Fusarium head blight in wheat. In: Leslie JF, Logrieco AF (eds) Mycotoxin reduction in grain chains. Wiley Blackwell, New Delhi, pp. 189–208Google Scholar
- Moerck KE, McElfresh P, Wohlman A, Hilton B (1980) Aflatoxin destruction in corn using sodium bisulfite, sodium hydroxide and aqueous ammonia. J Food Prot 43:571–574Google Scholar
- Moss MO (1971) The rubratoxins, toxic metabolites of Penicillium rubrum Stoll. In: Ciegler A, Kadis S, Ayl SJ (eds) Mycrobial toxins 6. Academic, London, pp. 381–497Google Scholar
- Mounjouenpou P, Mbang JA, Guyot B, Guiraud JP (2012) Traditional procedures of cocoa processing and occurrence of ochratoxin-a in the derived products. J Chem Pharm Res 4:1332–1339Google Scholar
- Müller HM (1983) Entgiftung von Mykotoxinen: II. Chemische Verfahren und Reaktion mit Inhaltsstoffen von Futtermitteln. Übers Tierernährg 11:47–80Google Scholar
- Munkvold GP (2014) Crop management practices to minimize the risk of mycotoxins contamination in temperate-zone maize. In: Leslie JF, Logrieco AF (eds) Mycotoxin reduction in grain chains. Wiley Blackwell, New Delhi, pp. 59–77Google Scholar
- Muthaiyan MC (2009) Plant quarantine treatments. In: Muthaiyan MC (ed) Principles and practices of plant quarantine. Allied Publishers, New Delhi, pp. 650–739Google Scholar
- NTP, US National Toxicology Program (2000) NTP technical report on the toxicology and carcinogenesis studies of fumonisin B1 (CAS No 116355–83-0) in F344/N Rats and B6C3F1 Mice (Feed Studies) (TR 496; NIH Publication No 99–3955). Research Triangle Park, NCGoogle Scholar
- Okonko PO, Nwokolo C (1978) Aflatoxin B1: simple procedures to reduce levels in tropical foods. Nutr Rep Int 17:387–395Google Scholar
- Rogers TD, Hitchens GD, Salinas CE, Murphy OG, Whitford HW (1992) Water purification, microbiological control, sterilization and organic waste decomposition using an electrochemical advanced ozonation process. SAE Technical Paper No. 921234. doi: 10.4271/921234
- Samarajeewa U, Sen AC, Cohen MD, Wei CI (1990) Detoxification of aflatoxins in foods and feeds by physical and chemical methods. J Food Prot 53:489–501Google Scholar
- Saunders D, Meredith F, Voss K (2001) Control of fumonisin: effects of processing. EnvironHealth Perspect 109:333–336Google Scholar
- SCF, Scientific Committee for Food (2002) Opinion of the Scientific Committee on Food on Fusarium toxins. Part 6: Group evaluation of T-2 toxin, HT-2 toxin, nivalenol and deoxynivalenol (adopted on 26 February 2002). http://ec.europa.eu/food/fs/sc/scf/out123_en.pdf. Accessed 16 July 2016
- SCF, Scientific Committee for Food (2003) Updated opinion of the Scientific Committee on Food on fumonisin B1, B2 and B3. http://ec.europa.eu/food/fs/sc/scf/out185_en.pdf. Accessed 16 July 2016
- Schwake-Anduschus C, Langenkämper G, Unbehend G, Dietrich R, Märtlbauer E, Münzing K (2010) Occurrence of Fusarium T-2 and HT-2 toxins in oats from cultivar studies in Germany and degradation of the toxins during grain cleaning treatment and food processing. Food Addit Contam 27:1253–1260CrossRefGoogle Scholar
- Schwartz PB, Casper HH, Beattie S (1995) Fate and development of naturally occurring Fusarium mycotoxins during malting and brewing. J Amer Soc Brew Chem 53:121–127Google Scholar
- Schwarz PB, Hill NS, Rottinghaus GE (2007) Fate of ergot (Claviceps purpurea) alkaloids during malting and brewing. J Am Soc Brew Chem 65:1–8Google Scholar
- Scott PM (1996) Mycotoxins transmitted into beer from contaminated grains during brewing. J Assoc Off Anal Chem Int 79:875–882Google Scholar
- Scott PM, Lawrence GA (1982) Losses of ergot alkaloids during making of bread and pancakes. J Agric Food Chem 30(3):445–450Google Scholar
- Scott PM, Lawrence GA (1994) Stability and problems in recovery of fumonisins added to corn-based foods. J Assoc Off Anal Chem Int 77:541–545Google Scholar
- Scott PM, Lawrence GA (1995) Analysis of beer for fumonisins. J Food Protect 58:1379–1382Google Scholar
- Slope SL, Wu S, Wu Z, Shen H, Chang XJ (2013) Patent CN 103205311B. A method for industrially removing zearalenone out of maize germ. State Grain Administration Institute (Granted 24 Dec 2014)Google Scholar
- Speijers GJA, van Egmond HP (1993) World-wide ochratoxin a levels in food and feeds. In: Creppy E, Castegnaro M, Dirheimer G (eds) Human Ochratoxicosis and its pathologies. John Libbey Eurotext Ltd., Paris, pp. 85–100Google Scholar
- Sreenivasamurthy V, Parpia HAB, Srikanta S, Shankarmurti A (1967) Detoxification of aflatoxin in peanut meal by hydrogen peroxide. J Assoc Off Anal Chem Int 50:350–354Google Scholar
- di Stefano V, Pitonzo R, Avellone G (2014) Effect of gamma irradiation on aflatoxins and ochratoxin a reduction in almond samples. J Food Res 3:113–118Google Scholar
- Suman M, Generotti S, Cirlini M, Malachova A, Berthiller F, Dall’Asta C (2014) Fate of mycotoxins along industrial biscuits and rusks processing, with deoxynivalenol and ochratoxin a as main targets, Proceedings of the International Mycotoxin Conference IMC2014 - Beijing, China, 19 to 23 May 2014Google Scholar
- Taylor MC, Jackson CJ, Tattersall DB, French N, Peat TS, Newman J, Briggs LJ, Lapalikar GV, Campbell PM, Scott C, Russell RJ, Oakeshott JG (2010) Identification and characterization of two families of F420 H2-dependent reductases from Mycobacteria that catalyse aflatoxin degradation. Mol Microbiol 78:561–575PubMedPubMedCentralCrossRefGoogle Scholar
- ten Bosch L, Avramidis G, Döll K, Wienecke S, Karlovsky P, Viöl W (2014) Degradation of Fusarium mycotoxins deoxynivalenol and zearalenone by cold atmospheric pressure plasma. http://www.mycotoxin-workshop.de/36th_Mycotoxin_Workshop_2014_-_Proceedings.pdf. Accessed 16 July 2016
- Teniola OD, Addo PA, Brost IM, Farber P, Jany KD, Jany KD, Alberts JF, van Zyl WH, Steyn PS, Holzapfel WH (2005) Degradation of aflatoxin B-1 by cell-free extracts of Rhodococcus erythropolis and Mycobacterium fluoranthenivorans sp. nov. DSM44556(T). Int J Food Microbiol 105:111–117PubMedCrossRefGoogle Scholar
- Velazhahan R, Vijayanandraj S, Vijayasamundeeswari A, et al. (2010) Detoxification of aflatoxins by seed extracts of the medicinal plant, Trachyspermum ammi (L.) Sprague ex Turrill—structural analysis and biological toxicity of degradation product of aflatoxin G1. Food Control 21:719–725. doi: 10.1016/j.foodcont.2009.10.014 CrossRefGoogle Scholar
- Wang JS, Luo H, Billam M, Wang Z, Guan H, Tang L, Goldston T, Afriyie-Gyawu E, Lovett C, Griswold J, Brattin B, Taylor RJ, Huebner HJ, Phillips TD (2005) Short-term safety evaluation of processed calcium montmorillonite clay (NovaSil) in humans. Food Addit Contam 22:270–279PubMedCrossRefGoogle Scholar
- Wang P, Afriyie-Gyawu E, Tang Y, Johnson NM, Xu L, Tang L, Huebner HJ, Ankrah NA, Ofori-Adjei D, Ellis W, Jolly PE, Williams JH, Wang JS, Phillips TD (2008) NovaSil clay intervention in Ghanaians at high risk for aflatoxicosis: II. Reduction in biomarkers of aflatoxin exposure in blood and urine. Food Addit Contam 25:622–634CrossRefGoogle Scholar
- Whitaker TB, Richard JL, Giesbrecht FG, Slate AB, Ruiz N (2003) Estimating deoxynivalenol in shelled corn barge lots by measuring deoxynivalenol in corn screenings. J Assoc Off Anal Chem Int 86:1187–1192Google Scholar
- Whitehurst RJ, van Oort M (2010) Enzymes in food technology. Wiley-Blackwell, SingaporeGoogle Scholar
- Wood GM (1982) Effects of processing on mycotoxins in maize. Chem Ind 972:1982Google Scholar
- Yagen B, Hutchins JE, Cox RH, Hagler WM, Hamilton PB (1989) Aflatoxin E1S: revised structure for the sodium sulfonate formed by destruction of aflatoxin B1 with sodium sisulfite. J Food Prot 52:574–577Google Scholar
- Yahl KR, Watson SA, Smith RJ, Barabolok R (1971) Laboratory wet-milling of corn containing high levels of aflatoxin and a survey of commercial wet-milling products. Cereal Chem 48:385–391Google Scholar
- Yoshizawa T, Takeda H, Ohi T (1983) Structure of a novel metabolite from deoxynivalenol, a trichothecene mycotoxin, in animals. Agric Biol Chem 47:2133–2135Google Scholar
- Yousef AE, Marth EH (1989) Stability and degradation of aflatoxin M1. In: Egmond HP (ed) Mycotoxins in dairy products. Elsevier, London, pp. 127–162Google Scholar
- Zhang H, Li J (1994) Detoxification of moniliformin. Acta Microbiol Sin 34:119–123Google Scholar
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