Abstract
Random amplified polymorphic DNA (RAPD)-PCR was employed to analyze the genetic diversity within 89 Saccharum spontaneum accessions collected from five different ecological regions of Guangxi Province, China. Of the 87 bands generated by nine RAPD primers, 96.6% were polymorphic. A substantial degree of genetic diversity was found among the locally collected S. spontaneum accessions. Pairwise genetic distance coefficients between accessions ranged from 8.6% (between accessions 87-10 and Yunge ③) to 74.2% (between accessions 87-31 and 85-53) with an average of 39.9%. Based on these genetic distance coefficients, the 89 S. spontaneum accessions were clustered into four groups irrespective of their ecological regions using the Cluster program of SAS (V8) software. Information from the present study would be useful for the conservation of locally collected S. spontaneum germplasm and exploitation of its genetic potential during sugarcane crossing program.
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References
Anonymous (2003) SAS Institute. SAS@9.1 for Windows. Cary, NC. Tai PYP, Miller JD, Legendre BL (1995) Evaluation of the world collection of Saccharum spontaneum L. Proc. Int. Soc. Sugar Cane Technol 21:250–260.
Burner DM, Legendre B L (1993) Sugarcane genome amplification for the subtropics: a 20-year effort. Sugar Cane 3:5–10.
Fan YH, Chen H, Shi XW, Xai Q, Zhang M, Zhang YP (2001). RAPD analysis of Saccharum spontaneum from different ecospecific colonies in Yunnan. Acta Botanica Yunnanica 23(3):298–308.
Nair NV, Jebadhas AW, Sreenivasan TV (1993) Saccharum germplasm collections in Arunachal Pradesh. Ind. J. Plant Genet. Resources, 6(l):21–26.
Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76:5269–5273.
Pan YB, Burner DM, Legendre BL, Grisham MP, White WH (2004) An assessment of the genetic diversity within a collection of Saccharum spontaneum L. with RAPD-PCR. Genetic Resources and Crop Evolution 51:895–903.
Mary S, Nair NV, Chaturvedi PK, Selvi A(2006) Analysis of genetic diversity among Saccharum spontaneum L.from four geographical regions of India, using molecular markers. Genetic Resources and Crop Evolution 53:1221–1231.
Tai PVP, Miller JD, Legendre BL (1995). Evaluation of the world collection of Saccharum spontaneum L. Proc. Int. Soc. Sugar Cane Technol. 21: 250–260.
Tan YM, Zeng JQ, Liang YB (1990) Analysis of the diversity of esterase isozymes of Guangxi S. spontaneum in relation to geographic distributions. Guangxi Sugarcane 4:14–18.
Tew TL (2003) World sugarcane variety census-Year 2000. Sugar Cane Intl. March/April 2003, pp.12–18.
Zhang GM, Yang RZ, Liu HB, Fang WK (2006) Principal component analysis for 7 quantitative traits and cluster analysis based on 7 quantitative traits of S. spontaneum L. Southwest China Journal of Agricultural Sciences 19(6):1127–1131.
Zhou YH, Huang HN, Li JF, Yang YH, Fu C (1996) Studies on characteristics of sugarcane germplasm at Hainan Sugarcane Breeding Station. Sugarcane and Canesugar 2:5–10.
Zhou YH, Huang HN, Li JF, Yang YH, Fu C (1997) Studies on characteristics of sugarcane germplasm at Hainan Sugarcane Breeding Station. Sugarcane and Canesugar 3:1–7.
Zhou YH, Huang HN, Li JF, Yang YH, Fu C (1999) Studies on characteristics of sugarcane germplasm at Hainan Sugarcane Breeding Station. Sugarcane and Canesugar 2:1–10.
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Zhang, Gm., Li, YR., He, WZ. et al. Analysis of the genetic diversity in Saccharum spontaneum L. accessions from Guangxi Province of China with RAPD-PCR. Sugar Tech 12, 31–35 (2010). https://doi.org/10.1007/s12355-010-0007-7
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DOI: https://doi.org/10.1007/s12355-010-0007-7