Abstract
Saccharum spontaneum L. a wild relative of sugarcane is the most variable and diverse among the Saccharum species. This species had been successfully exploited in sugarcane improvement programmes since 1915 and most of the present day commercial varieties are derivatives of interspecific hybrids involving S. spontaneum. The S. spontaneum germplasm available today in the World collections is diverse and represent different geographical groups. In the present investigation, an attempt was made to characterize 40 S. spontaneum clones collected from 4 different geographical areas in India using 20 random, 2 ISSR and 2 telomere primers. Of the 491 bands generated by these primers 83.9% were polymorphic. The genetic diversity estimated based on these markers was found to be moderate (48.9%). The pair-wise genetic distance between the accessions ranged from 29.8 to 60.0. The accessions from Arunachal Pradesh were found to be the most diverse, while Tamil Nadu accessions showed relatively less diversity. Diversity between Tamil Nadu and Kerala collections was found to be low, while the diversity between the Orissa group and the rest was found to be high. The collections from Mayurbanj and Cuttack regions of Orissa were found to be distinct. Arunachal Pradesh accessions, being more diverse, are a potential source for exploitation in sugarcane breeding programmes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
S.M. Al-Janabi, M. Mc Clelland, C. Peterson and B.W.S. Sobral, Phylogenetic analysis of organellar DNA sequences in the Andropogoneae; Saccharinae. Theor. Appl. Genet. 88 (1994) 933-944
P. Besse, G. Taylor, B. Carrol, N. Berding, D. Burner and C.L. Mc Intyre, Assessment of genetic diversity in a sugarcane Germplasm Collection using an automated AFLP analysis. Genetica 104 (1998) 143-153
W.L. Burnquist, M.E. Sorrels and S.D. Tankesly, Characterization of genetic variability in Saccharum germplasm by means of Restriction Fragment Length Polymorphism (RFLP) analysis. Proc. Int. Soc. Sugarcane Technol. 21 (1995) 355-365
H. Chen, Y.H. Fan, X.W. Shi, Q. Cai, M. Zhang and Y.P. Zhang, Research on genetic diversity and systemic evolution in Saccharum spontaneum L. Acta Agronomica Sinicia. 27 (2001) 645-652
G.M. Cordiero, G.O. Taylor and R.J. Henry, Characterization of microsatellite markers from sugarcane (Saccharum sp.). Plant Sci. 155 (2000) 161-168
O. Coto, M.T. Cornide, D. Calvo, E. Canales, A. D'hont and F. de Prada, Genetic diversity among wild sugarcane germplasm from Laos revealed with markers. Euphytica 123 (2002) 121-130
A. D' Hont, Y.H. Lu, P. Feldmann and J.C. Glaszmann, Cytoplasmic diversity in sugarcane revealed by heterologous probes. Sugarcane 1 (1993) 12-15
A. D' Hont, L. Grivet, P. Feldmann, P. Rao, N. Berding and Glaszmann, Characterization of the double genome structure of modern sugarcane cultivars (Saccharum spp.) by molecular cytogenetics. Mol. Gen. Genet. 250 (1996) 405-413
Y.H. Fan, H. Chen, X.W. Shi, Q. Xai, M. Zhang and Y.P. Zhang, RAPD analysis of Saccharum spontaneum from different ecospecific colonies in Yunnan. Acta Botanica Yunnanica 23 (2001) 298-308
H. Harvey, B.I. Huckett and F.C. Botha, Use of Polymerase Chain Reaction and Random Amplification of Polymorphic DNAs for the determination of genetic distances between 21 sugarcane varieties. Proc. South Afr. Sugar Technol. Assocn. 68 (1994) 36-40
M. Harvey and F.C. Botha, Use of PCR based methodologies for determination of DNA diversity between Saccharum varieties. Euphytica 89 (1996) 257-265
P.A. Kandasami, T.V. Sreenivasan, T.C. Ramana Rao, K. Palanichami, B.V. Natarajan, K.C. Alexander, M.M. Rao and D. Mohan Raj, Catalogue on Sugarcane Genetic Resources I. S. spontaneum. Coimbatore: Sugarcane Breeding Institute (ICAR) (1983).
M.L.A. Lima, A.A.F. Garcia, K.M. Oliveira, S. Matisouka, H. Arizono, C.L. de Souza Jr. and A.P. de Souza, Analysis of genetic similarity detected by AFLP and coefficient of parentage among genotypes of sugarcane (Saccharum spp.). Theor. Appl. Genet. 104 (2002) 30-38
Y.H. Lu, A. D'Hont, D.I.T. Walker, P.S. Rao, P. Feldmann and J.C. Glaszmann, Relationships among ancestral species of sugarcane revealed with RFLP using single copy maize nuclear probes. Euphytica 78 (1994) 7-18
S.K. Mukherjee, Origin and distribution of Saccharum. Bot. Gaz. I (1957) 55-61
N.V. Nair, A.W. Jebadhas and T.V. Sreenivasan, Saccharum germplasm collections in Arunachal Pradesh. Ind. J. Pl. Genet. Resources. 6 (1993) 21-26
N.V. Nair, S. Nair, T.V. Sreenivasan and M. Mohan, Analysis of genetic diversity and phylogeny in Saccharumrelated genera using RAPD markers. Genet. Resour. Crop Evol. 46 (1999) 73-79
N.V. Nair, A. Selvi, T.V. Sreenivasan and K.N. Pushpalatha, Molecular diversity in Indian sugarcane cultivars as revealed by randomly amplified polymorphisms. Euphytica 127 (2002) 219-225
M. Nei, Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89 (1978) 583-590
R.R. Panje, The role of S. spontaneum in sugarcane breeding. Proc. Int. Soc. Sugarcane Technol. 14 (1972) 217-223
N. Parthasarathy, Origin of noble sugarcanes (Saccharum officinarum L). Nature 161 (1946) 608
D. Pattanayak, S.K. Chakrabarti and P.S. Naik, Genetic diversity of late blight resistant and susceptible Indian potato cultivars revealed by RAPD markers. Euphytica 128 (2002) 183-189
B.T. Roach, Utilization of Saccharum spontaneum in sugarcane breeding. Proc. Int. Soc. Sugarcane Technol. 16 (1978) 43-57
B.T. Roach and J. Daniels, A review of the origin and improvement of sugarcane. Proc. Copersucar Intern. Brazil: Sugarcane Breeding Workshop (1987).
F.J. Rohlf, NTSYS-PC- Numerical Taxonomy and Multivariate Analysis System. Version 1.8. SetauketNew York: Exeter publications (1993).
B.W.S. Sobral, D.P.V. Braga, E.S. La Hood and P. Keim, Phylogenetic analysis of chloroplast restriction site mutations in the Saccharinae (Griesb.) of the Andropogoneae Dumort. tribe. Theor Appl Genet. 87 (1994) 843-853
T.V. Sreenivasan, K. Palanichami and M.N. Koppar, Saccharum germplasm collection from Arunachal PradeshIndia. Sugarcane 5 (1986) 13-14
T.V. Sreenivasan, V.A. Amalraj and A.W. Jebadhas, Sugarcane Genetic Resources V. Saccharum spontaneum L. vol. 2. Coimbatore: Sugarcane Breeding Institute(ICAR) (2001).
V. Walbot, Preparation of DNA from single rice seedlings. Rice Genet. News. l5 (1988) 149-151
J.G.K. Williams, A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey, DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18 (1990) 6531-6535
M.Q. Zhang, A.L. Yu and R.K. Chen, Utility of SSRs for determining genetic similarities and relationships in Saccharumrelated genera. Proc. Int. Soc. Sugarcane Technol. 24 (2001) 630-631
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mary, S., Nair, N.V., Chaturvedi, P. et al. Analysis of Genetic Diversity Among Saccharum spontaneum L. From Four Geographical Regions of India, Using Molecular Markers. Genet Resour Crop Evol 53, 1221–1231 (2006). https://doi.org/10.1007/s10722-005-2433-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10722-005-2433-z