Abstract
Genetic diversity among sugarcane hybrids (Saccharum spp) is pre-requisite for sugarcane improvement through breeding. Twelve decamer oligonucleotide random-amplified polymorphic DNA (RAPD) markers were utilized to investigate the genetic potential among 24 sugarcane cultivars. A total of 120 fragments were originated by 12 RAPD primers. An average number of fragments were obtained as 11.42 fragments per cultivar, which ranged from 4 to 21 fragments. The genetic similarity among 24 sugarcane cultivars ranged from 0.236 to 0.944 with the mean similarity value of 0.508. On the basis of phylogenetic analysis based on dendrogram, the cultivars were clustered into five groups. Two varieties Co 0118 and CoS 07250 were found as highly diverse sugarcane cultivars. Three most popular cultivars viz, Co 0238, Co 1158, and CoS 08272 were clustered a diverse among particular group. These clusters with their diverse genealogy indicated the influence of parental genome contribution to clustering. Diverse varieties developed for east region were grouped in the separate clusters which indicated the influence of adaptation of varieties to particular agro-climatic condition. Hence, these five diverse hybrid cultivars would be used in further breeding program to get the prominent sugarcane clones which may produced higher cane yield and sugar content.
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References
Afghan S, Haider MS, Shah AH, Rashid N, Iqbal J, Tahir M, Akhtar M (2005) Detection of genetic diversity among sugarcane (Saccharum sp.) genotypes using random amplified polymorphic DNA markers. Sugar Cane Int 23:15–19
Ahmed, Gardezi SD (2017) Molecular characterization of locally adopted sugarcane (Saccharum Officinarum L.) varieties using microsatellite markers. J Anim Plant Sci 27(1):164–174
Alvi AK, Iqbal J, Shah AH, Pan YB (2008) DNA based genetic variation for red rot resistance in sugarcane. Pak J Bot 40(2):1419–1425
Besse P, McIntyre CL (1998) Isolation and characterization of repeated DNA sequences from Erianthus spp. (Saccharinae: Andropogoneae). Genome 41:408–416
Cunff LL, Garsmeur O, Raboin LM, Pauquet J, Telismart H, Selvi A, Grivet L, Philippe R, Begum D, Deu M, Costet L, Wing R, Glaszmann JC, D’Hont A (2008) Diploid/polyploid syntenic shuttle mapping and haplotype-specific chromosome walking towards a rust resistance gene (BruI) in highly polyploid sugarcane (2n = 12 × 115). Genetics 180:649–660
D’Hont A, Grivet L, Feldmann P, Glaszmann JC, Rao S and Berding N (1996) Characterisation of the double genome structure of modern sugarcane cultivars (Saccharum spp.) by molecular cytogenetics. Mol General Gene 250:405–413
Goel P, Swati Sharma PK, Srivastava K (2005) Genetic divergence in an elite germplasm collection of wheat (Triticum spp.). Crop Improv 32:114–120
Hemaprabha G, Natarajan US, Balasundaram N, Singh NK (2006) STMS based genetic divergence among common parents and its use in identifying productive cross combinations for varietal evolution in sugarcane (Saccharum sp.). Sugar Cane Int 24:22–27
Jisen Z, Marvellous Z, James W, Ming R (2013) Sugarcane genetics and genomics. In Sugarcane: physiology, biochemistry, and functional biology, pp 623–643
Mohammadi SA, Prasanna BM (2003) Review and interpretation analysis of genetic diversity in crop plants—Salient Statistical Tools. Crop Sci 43:1235–1248
Nair NV, Selvi A, Sreenivasan TV, Pushpalatha KN (2002a) Molecular diversity in Indian sugarcane varieties as revealed by randomly amplified DNA polymorphisms. Euphytica 127:219–225
Nair NV, Selvi A, Sreenivasan TV, Pushpalatha KN (2002b) Molecular diversity in Indian sugarcane cultivars as revealed by the random amplified DNA polymorphisms. Euphytica 127:219–225
Pan YB, Burner DM, Legendre BL, Grisham MP, White WH (2004) An assessment of genetic diversity within a collection of Saccharum spontaneum L. with RAPD-PCR. Genet Resour Crop Evol 51:895–903
Piperidis G (2003) Progress towards evaluation of SSR as a tool for sugarcane variety identification. In: ISSCT. IV. Mol. Biol. Workshop, Montpellier, France
Roach BT (1978) Utilization of Saccharum spontaneum in sugarcane breeding. Proc Internat Soc Sugarcane Technol 16:43–58
Rossi M, Araujo PG, Paulet F, Garsmeur O, Dias VM, Chen H, Van Sluys MA, D’Hont AD (2003) Genomic distribution and characterization of EST-derived resistance gene analogs (RGAs) in sugarcane. Mol Genet Genom 269:406–419
Saravanakumar K, Govindaraj P, Appunu C, Senthilkumar S, Kumar R (2014) Analysis of genetic diversity in high biomass producing sugarcane hybrids (Saccharum spp. complex) using RAPD and STMS markers. Indian J Biotechnol 13:214–220
Selvi A, Nair NV, Balasundaram N, Mohapatra T (2003) Evaluation of maize microsatellite markers for genetic diversity analysis and fingerprinting in sugarcane. Genome 46:394–403
Selvi A, Nair NV, Noyer JL, Singh NK, Balasundaram N, Bansal KC, Koundal KR, Mohapatra T (2005a) Genomic constitution and genetic relationship among the tropical and subtropical Indian sugarcane cultivars revealed by AFLP. Crop Sci 45:1750–1757
Selvi A, Nair NV, Noyer JL, Singh NK, Balasundaram N, Bansal KC, Koundal KR, Mohapatra T (2005b) Genomic constitution and genetic relationship among the tropical and subtropical Indian sugarcane cultivars revealed by AFLP. Crop Sci 45:1750–1755
Sharma MS, Dobhal U, Singh P, Kumar S, Gaur AK, Singh SP, Jeena AS, Koshy EP, Kumar S (2014) Assessment of genetic diversity among sugarcane cultivars using novel microsatellite markers. Afr J Biotechnol 13(13):1444–1451
Singh SP, Nigam A, Singh RK (2015) Significant role of molecular markers in sugarcane improvement. Agrica 4(2):67–78
Sreenivasan TV, Ahloowalia BS, Heinz DJ (1987) Cytogenetics. In: Heinz DJ (ed) Sugarcane improvement through breeding. Elsevier, Amsterdam, pp 211–253
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Singh, P., Singh, S.P., Tiwari, A.K. et al. Genetic diversity of sugarcane hybrid cultivars by RAPD markers. 3 Biotech 7, 222 (2017). https://doi.org/10.1007/s13205-017-0855-x
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DOI: https://doi.org/10.1007/s13205-017-0855-x