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Construction of recombinant Corynebacterium glutamicum for L-threonine production

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Abstract

L-threonine is an essential amino acid which is widely used in feed and pharmaceutical industries. We recently engineered Corynebacterium glutamicum R102 (AHVr) for improved production of L-threonine. Inactivation of genes metX and dapA encoding dihydrodipicolinate synthase and homoserine O-acetyltransferase, respectively, was firstly conducted by homologous recombination, which differed from the common random mutagenesis method. Then operon gene hom-thrB (O) and export gene thrE (E) from R102 were over-expressed alone or together to obtain a series of recombinant strains. qPCR was employed to evaluate the transcript quantification of the target genes. In flask fermentation, the newly constructed strain R102Δ metXΔdapA (pEC-Box) was able to accumulate 3.35 g threonine/L compared with 1.80 g threonine/L of strain R102 (AHVr).

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Authors and Affiliations

  1. Department of bioengineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510-006, China

    Yangyong Lv, Zhanhong Wu, Shuangyan Han, Ying Lin & Suiping Zheng

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  1. Yangyong Lv
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  2. Zhanhong Wu
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  3. Shuangyan Han
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  4. Ying Lin
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  5. Suiping Zheng
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Correspondence to Suiping Zheng.

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Lv, Y., Wu, Z., Han, S. et al. Construction of recombinant Corynebacterium glutamicum for L-threonine production. Biotechnol Bioproc E 17, 16–21 (2012). https://doi.org/10.1007/s12257-011-0360-8

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  • DOI: https://doi.org/10.1007/s12257-011-0360-8

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