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Construction of Synthetic Promoter-Based Expression Cassettes for the Production of Cadaverine in Recombinant Corynebacterium glutamicum

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Abstract

Corynebacterium glutamicum is an important microorganism in the biochemical industry for the production of various platform chemicals. However, despite its importance, a limited number of studies have been conducted on how to constitute gene expression cassettes in engineered C. glutamicum to obtain desired amounts of the target products. Therefore, in this study, six expression cassettes for the expression of the second lysine decarboxylase of Escherichia coli, LdcC, were constructed using six synthetic promoters with different strengths and were examined in C. glutamicum for the production of cadaverine. Among six expression cassettes, the expression of the E. coli ldcC gene under the PH30 promoter supported the highest production of cadaverine in flask and fed-batch cultivations. A fed-batch fermentation of recombinant C. glutamicum expressing E. coli ldcC gene under the PH30 promoter resulted in the production of 40.91 g/L of cadaverine in 64 h. This report is expected to contribute toward developing engineered C. glutamicum strains to have desired features.

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Acknowledgments

This work was supported by Industrial Strategic Technology Development Program (10047910, Production of bio-based cadaverine and polymerization of Biopolyamide 510) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea) and the Technology Development Program to Solve Climate Changes (Systems Metabolic Engineering for Biorefineries) from the Ministry of Science, and ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012-C1AAA001-2012M1A2A2026556). Further support from the R&D Program of MOTIE/KEIT (10049674) is appreciated.

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    Authors and Affiliations

    1. Center for Bio-based Chemistry, Green Chemistry & Engineering Division, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-600, Republic of Korea

      Young Hoon Oh, Jae Woo Choi, Eun Young Kim & Bong Keun Song

    2. Department of Biological and Chemical Engineering, Hongik University, Chungnam, 339-701, Republic of Korea

      Jae Woo Choi & Kyungmoon Park

    3. Department of Chemical and Biomolecular Engineering (BK21 Plus Program) and Institute for the BioCentury, KAIST, 335 Gwahagno, Yuseong-gu, Daejeon, 305-701, Republic of Korea

      Ki Jun Jeong

    4. Bio R&D Center, Paik Kwang Industry Co., 57 Oehang-4 Gil, Gunsan-si, Jeollabukdo, 573-879, Republic of Korea

      Il-Kwon Kim

    5. Clean Energy Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 136-791, Republic of Korea

      Han Min Woo

    6. Department of Biotechnology and Bioengineering, College of Engineering, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 500-757, Republic of Korea

      Seung Hwan Lee

    7. Department of Environmental Engineering and Energy, Myongji University, 116 Myongji-ro, Cheoin-gu, Yongin, Gyeonggido, 449-728, Republic of Korea

      Si Jae Park

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    1. Young Hoon Oh
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    2. Jae Woo Choi
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    3. Eun Young Kim
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    5. Ki Jun Jeong
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    6. Kyungmoon Park
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    8. Han Min Woo
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    9. Seung Hwan Lee
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    10. Si Jae Park
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    Correspondence to Seung Hwan Lee or Si Jae Park.

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    Young Hoon Oh and Jae Woo Choi contributed equally to this work.

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    Oh, Y.H., Choi, J.W., Kim, E.Y. et al. Construction of Synthetic Promoter-Based Expression Cassettes for the Production of Cadaverine in Recombinant Corynebacterium glutamicum . Appl Biochem Biotechnol 176, 2065–2075 (2015). https://doi.org/10.1007/s12010-015-1701-4

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    • DOI: https://doi.org/10.1007/s12010-015-1701-4

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