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Isolation and characterization of algicidal bacteria from Cochlodinium polykrikoides culture

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Abstract

In this study, we analyzed a bacterial community closely associated with Cochlodinium polykrikoides that caused harmful algal blooming in the sea. Filtration using a plankton mesh and percoll gradient centrifugation were performed to eliminate free-living bacteria. Attached bacteria were analyzed by culture-dependent and culture-independent methods. Five culturable bacterial strains were isolated and identified from the C. polykrikoides mixed bacterial community. The isolates belonged to α-Proteobacteria (Nautella sp., Sagittula sp., and Thalassobius sp.) and γ-Proteobacteria (Alteromonas sp. and Pseudoalteromonas sp.). All of the 5 isolates showed algicidal activity against C. polykrikoides and produced extracellular compounds responsible for algicidal properties after entering the stationary phase. The algicidal compounds produced by the 5 isolates were heat-stable and had molecular masses of less than 10,000 Da. Furthermore, the algicidal compounds were relatively specific for C. polykrikoides in terms of their algicidal activities. Culture-independent analysis of the bacterial community in association with C. polykrikoides was performed using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). On the basis of the PCR-DGGE profile, Sagittula sp. was identified as a dominant species in the bacterial community of C. polykrikoides.

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Authors and Affiliations

  1. Department of Microbiology, Pusan National University, Busan, 609-735, Korea

    Jeong-Il Oh, Min-Ju Kim & Ji-Young Lee

  2. Korea Science Academy of KAIST, Busan, 614-822, Korea

    In-Jeong Ko

  3. Department of Environmental Engineering, Pioneer Research Center for Controlling of Harmful Algal Bloom, Chosun University, Gwangju, 501-759, Korea

    Wonduck Kim & Si Wouk Kim

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  1. Jeong-Il Oh
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Correspondence to Si Wouk Kim.

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Oh, JI., Kim, MJ., Lee, JY. et al. Isolation and characterization of algicidal bacteria from Cochlodinium polykrikoides culture. Biotechnol Bioproc E 16, 1124–1133 (2011). https://doi.org/10.1007/s12257-011-0232-2

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  • DOI: https://doi.org/10.1007/s12257-011-0232-2

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