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Producing mechanism of an algicidal compound against red tide phytoplankton in a marine bacterium γ-proteobacterium

  • Applied Microbial and Cell Physiology
  • Published:
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Abstract

Strain MS-02-063, γ-proteobacterium, isolated from a coast area of Nagasaki, Japan, produced a red pigment which belongs to prodigiosin members. This pigment, PG-L-1, showed potent algicidal activity against various red tide phytoplanktons in a concentration-dependent manner. An understanding of a mechanism of PG-L-1 production by this marine bacterium may yield important new insights and strategies for preventing blooms of harmful flagellate algae in natural marine environments. Therefore, we analyzed the mechanisms of PG-L-1 production. In our previous study, the pigment production by this marine bacterium was completely inhibited at 1.56 μg/ml of erythromycin or 3.13 μg/ml of chloramphenicol, while minimal inhibitory concentrations for cell growth of erythromycin and chloramphenicol against this bacterium were >100 and 25 μg/ml, respectively. It is interesting to note that the ability of the pigment production in erythromycin-treated bacterium recovered by an addition of homoserine lactone. In fact, the pigment production was inhibited by β-cyclodextrin that inhibits autoinducer activities by a complex with N-acyl homoserine lactones. N-acyl homoserine lactones with autoinducer activities are ubiquitous bacterial signaling molecules that regulate gene expression in a cell density dependent process known as quorum sensing. Therefore, it was suggested that PG-L-1 produced by strain MS-02-063 is controlled by the homoserine lactone quorum sensing. It is speculated that this quorum sensing is involved in the production of algicidal agents of other marine bacteria. This bacterium and other algicidal bacteria might be concerned in regulating the blooms of harmful flagellate algae through the quorum sensing system.

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Abbreviations

N-AHL:

N-acyl homoserine lactones

DAPI:

4,6-Diamidino 2-phenylindole dihydrochloride

ESM:

Erd–Schreiber modified

HABs:

Harmful algal blooms

HPLC:

High performance liquid chromatography

MIC:

Minimal inhibitory concentration

YPG medium:

Yeast extract-peptone-glucose medium

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Authors and Affiliations

  1. Division of Biochemistry, Faculty of Fisheries, Nagasaki University, Nagasaki, 852-8521, Japan

    Takuji Nakashima, Kenichi Yamaguchi & Tatsuya Oda

  2. Nagasaki Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, JST, 2-1308-8 Ikeda, Omura City, Nagasaki, 856-0026, Japan

    Takuji Nakashima & Yousuke Miyazaki

  3. National Research Institute of Fisheries and Environment of Inland Sea, Maruishi, Ohno, Saeki, Hiroshima, 739-0452, Japan

    Yukihiko Matsuyama

  4. Graduate School of Science and Technology, Nagasaki University, Nagasaki, 852-8521, Japan

    Wataru Muraoka

  5. Division of Biochemistry, Faculty of Fisheries, Bunkyo 1-14, Nagasaki, 852-8521, Japan

    Takuji Nakashima

Authors
  1. Takuji Nakashima
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  2. Yousuke Miyazaki
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  3. Yukihiko Matsuyama
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  4. Wataru Muraoka
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  5. Kenichi Yamaguchi
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  6. Tatsuya Oda
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Correspondence to Takuji Nakashima.

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Nakashima, T., Miyazaki, Y., Matsuyama, Y. et al. Producing mechanism of an algicidal compound against red tide phytoplankton in a marine bacterium γ-proteobacterium . Appl Microbiol Biotechnol 73, 684–690 (2006). https://doi.org/10.1007/s00253-006-0507-2

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  • DOI: https://doi.org/10.1007/s00253-006-0507-2

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