Abstract
A marine gliding bacterium Cytophaga sp. (strain J18/M01) was isolated from Harima-Nada, eastern Seto Inland Sea, Japan in 1990. This bacterium preys upon various species of marine phytoplankton. All of the five raphidophycean flagellates, all of the four diatoms, and one of the two dinoflagellates examined were killed within a few days when cultured with the bacterium. The bacterium presumably achieves this by direct attack, because the culture filtrate in which host organisms were totally destroyed had no significant effects on the growth of the same host organism (Chattonella antiqua). If one or a few bacterial cells were inoculated into C. antiqua culture, all of the host organisms were killed. The bacterium proliferated in filter-sterilized seawater, suggesting its ubiquitous existence in the coastal sea. The killing of phytoplankton by bacteria such as Cytophaga sp. J18/M01 may be a significant factor influencing the population dynamics of phytoplankton in nature and may contribute to the sudden disappearance of red tides in the coastal sea. Bacterial destruction of phytoplankton may also be a factor that regulates primary productivity in marine ecosystems.
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Literature cited
Alexander, M. (1981). Why microbial predators and parasites do not eliminate their prey and hosts. A. Rev. Microbiol 35:113–133
Ashton, P. J., Robarts, R. D. (1987). Apparent predation of Microcystis aeruginosa Kurz. Emend Elenkin by a Saprospira-like bacterium in a hypertrophic lake (Hartbeespoort dam, South Africa). J. Limnol. Soc. S. Afr. 13:44–47
Baker, K. H., Herson, D. S. (1978). Interactions between the diatom Thalassiosira pseudonana and an associated Pseudomonad in a mariculture system. Appl. envirl Microbiol. 35:791–796
Brand, L. E., Guillard, R. R. L. (1981). A method for the rapid and precise determination of acclimated phytoplankton reproduction rates. J. Planktor. Res. 3: 193–201
Brayton, P. R., Tamplin, M. L., Huq, A., Colwell, R. R. (1987). Enumeration of Vibrio cholerae O1 in Bangladesh waters by fluorescent-antibody direct viable count. Appl. envirl Microbiol. 53: 2862–2865
Buck, J. D., Cleverdon, R. C. (1960). The spread plate as a method for the enumeration of marine bacteria. Limnol. Oceanogr. 5: 78–80
Barnham, J. C., Collan, S. A., Daft, M. J. (1984). Myxococcal predation of the cyanobacterium Phormidium luridum in aqueous environments. Archs. Microbiol. 137: 220–225
Chen, L. C. M., Edelstain, T., McLachlan, J. (1969). Bonnemaisonia hamifera Hariot inmature and in culture. J. Phycol. 5: 211–220
Cole, J. J. (1982). Interactions between bacteria and algae in aquatic ecosystems. A. Rev. Ecol. Syst. 13: 291–314
Daft, M. J., McCord, S. B., Stewart, W. D. P. (1975). Ecological studies on algal-lysing bacteria in fresh waters. Freshwat. Biol. 5: 577–596
Dahle, A. B., Laake, M. (1982). Diversity dynamics of marine bacteria studied by immunofluorescent staining on membrane filters. Appl. envirl Microbiol. 43: 169–176
Fraleigh, P. C., Bunham, J. C. (1988). Myxococcal predation on cyanobacterial populations: nutrient effects. Limnol. Oceanogr. 33: 476–483
Fukami, K., Nishijima, T., Murata, H., Doi, S., Hata, Y. (1991). Distribution of bacteria influential on the development and the decay of Gymnodinium nagasakiense red tide and their effects on algal growth. Nippon Suisan Gakk 57: 2321–2326
Fukami, K., Yuzawa, A., Nishijima, T., Hata, Y. (1992). Isolation and properties of a bacterium inhibiting the growth of Gymnodinium nagasakiense. Nippon Suisan Gakk. 58: 1073–1077
Furuki, M., Kobayashi, M. (1991). Interaction between Chattonella and bacteria and prevention of this red tide. Mar. Pollut. Bull. 23: 189–193
Furuki, M., Moriguchi, Y., Kitamura, H. (1985). Effects of bacteria on formation of Chattonella sp. bloom in the Sea of Harima. Hakkokogaku 63: 61–69 (in Japanese with English abstract)
Imai, I. (1984). Size distribution and biomass of bacteria in Suo-Nada, western Seto Inland Sea. Bull. Nansei reg. Fish. Res. Lab. 17: 183–196 (in Japanese with English abstract)
Imai, I. (1987). Size distribution, number and biomass of bacteria in intertidal sediments and seawater of Ohmi Bay, Japan. Bull. Jap. Soc. microb. Ecol. 2: 1–11
Imai, I. (1989). Microbial ecology in coastal systems. Bull. cstl Oceanogr. 28: 85–101 (in Japanese with English abstract)
Imai, I., Ishida, Y., Sawayama, S., Hata, Y. (1991). Isolation of a marine gliding bacterium that kills Chattonella antiqua (Raphidophyceae). Nippon Suisan Gakk. 57: 1409
Imai, I., Itoh, K. (1987). Annual life cycle of Chattonella spp., causative flagellates of noxious red tides in the Inland Sea of Japan. Mar. Biol. 94: 287–292
Ishida, Y., Shibahara, K., Uchida, H., Kadota, H. (1980). Distribution of obligately oligotrophic bacteria in Lake Biwa. Bull. Jap. Soc. Scient. Fish. 46: 1151–1158
Ishio, S., Mangindaan, R. E., Kuwahara, M., Nakagawa, H. (1989). A bacterium hostile to flagellates: identification of species and characters. In: Okaichi, T., Anderson, D. M., Nemoto, T. (eds.) Red tides: biology, environmental science, and toxicology. Elsevier, New York, p. 205–208
Itoh, K., Imai, I. (1987). Rafido so (Raphidophyceae). In: Japan Fisheries Resource Conservation Association (ed.) A guide for studies of red tide organisms. Shuwa, Tokyo, p. 122–130 (in Japanese)
Kimura, B. (1989). Studies on chemical and biological factors influencing the growth of Uroglena americana, a red tide Chrysophyceae in Lake Biwa. J. Shimonoseki Univ. Fish. 38: 23–70
Knauer, G. A., Hebel, D., Cipriano, F. (1982). Marine snow: major site of primary production in coastal waters. Nature, Lond. 300: 630–631
Mitsutani, A., Uchida, A., Ishida, Y. (1987). Occurrence of bluegreen algae and algal lytic bacteria in Lake Biwa. Bull. Jap. Soc. microb. Ecol. 2: 21–28
Porter, K. G., Feig, Y. S. (1980). The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25: 943–948
Porter, K. G., Sherr, E. B., Sherr, B. F., Pace, M., Sanders, R. W. (1985). Protozoa in planktonic food webs. J. Protozool 32: 409–415
Reichenbach, H. (1981). Taxonomy of the gliding bacteria. A. Rev. Microbiol. 35: 339–364
Reim, R. L., Shane, M. S., Cannon, R. E. (1974). The characterization of a Bacillus capable of blue-green bactericidal activity. Can. J. Microbiol. 20: 981–986
Revelante, N., Gilmartin, M. (1991). The phytoplankton composition and population enrichment in gelatinous “macroaggregates” in the northern Adriatic during the summer of 1989. J. exp. mar. Biol. Ecol. 146: 217–233
Riquelme, C. E., Ishida, Y. (1989). Interaction between microalgae and bacteria in coastal seawater. Mem. Coll. Agr. Kyoto Univ. 134: 1–60
Safferman, R. S., Morris, M. E. (1963). Algal virus: isolation. Science, N.Y. 140: 679–680
Sakata, T. (1990). Occurrence of marine Saprospira sp. possessing algicidal activity for diatoms. Nippon Suisan Gakk. 56: 1165
Sakata, T., Fujita, Y., Yasumoto, H. (1991). Plaque formation by algicidal Saprospira sp. on a lawn of Chaetoceros ceratosporum. Nippon Suisan Gakk. 57: 1147–1152
Sherr, E. B., Sherr, B. F., Paffenhöfer, G.-A. (1986). Phagotrophic protozoa as food for metazoans: a “missing” trophic link in marine pelagic food webs? Mar. microb. Fd Webs 1: 61–80
Shilo, M. (1970). Lysis of blue-green algae by Myxobacter. J. Bact. 104: 453–461
Stewart, J. R., Brown, R. M. (1969). Cytophaga that kills or lyses algae. Science, N.Y. 164: 1523–1524
Stewart, W. D. P., Daft, M. J. (1977). Microbial pathogens of cyanophycean blooms. In: Droop, M. R., Jannasch, H. W. (eds.) Advances in aquatic microbiology, Vol. 1. Academic Press, London, p. 177–218
Throndsen, J. (1978). The dilution-culture method. In: Sournia, A. (ed.) Phytoplankton manual. Unesco, Paris, p. 218–224
Ukeles, R., Bishop, J. (1975). Enhancement of phytoplankton growth by marine bacteria. J. Phycol. 11: 142–149
Yamaguchi, M., Imai, I., Honjo, T. (1991). Effects of temperature, salinity and irradiance on the growth rates of the noxious red tide flagellates Chattonella antiqua und C. marina (Raphidophyceae). Nippon Suisan Gakk. 57: 1277–1284 (in Japanese with English abstract)
Yamamoto, Y. (1978). Detection of algal lysing biological agents in lakes by the soft-agar overlayer technique. Jap. J. limnol. 39: 9–14 (in Japanese with English abstract)
Yamamoto, Y. (1988). Cyanobacteria-lysing agents and their distribution patterns of lakes and river in Japan. Bull. Jap. Soc. microb. Ecol. 2: 77–88 (in Japanese with English abstract)
Yoshida, Y., Kawaguchi, K. (1983). Buoyancy and phototaxis of Chattonella antiqua (Hada) Ono. Bull. Plankton Soc., Japan 30: 11–19 (in Japanese with English abstract)
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Communicated by T. Ikeda, Niigata
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Imai, I., Ishida, Y. & Hata, Y. Killing of marine phytoplankton by a gliding bacterium Cytophaga sp., isolated from the coastal sea of Japan. Marine Biology 116, 527–532 (1993). https://doi.org/10.1007/BF00355470
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DOI: https://doi.org/10.1007/BF00355470