Abstract
The autofluorescence (primary fluorescence, AF) of agar cultures of the brown-rot fungus Piptoporus betulinus was investigated in Zeiss Jenalumar and Nikon Eclipse 8201 fluorescence microscopes at various excitations. The strongest AF of hyphae was found in minimal medium with glucose, where the hyphae exhibited green AF at violet (450 nm) excitation and red AF at green (570 nm) excitation. Addition of metals to cultivation media led to enhanced white-blue AF in the presence of Co (at 450 nm) and yellow to yellow-brown AF at 510 nm. When cultivated with Mn and Zn, enhanced AF of intracellular content was observed. Only a weak signal was found in the presence of Cu and Fe.
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Abbreviations
- AF:
-
autofluorescence
- CLN:
-
medium with cellulose and low nitrogen
- FITC:
-
fluorescein isothiocyanate
- GC:
-
glucose-corn steep medium
- ME:
-
maltose extract medium
- MM:
-
minimal medium with glucose
- ROS:
-
reactive oxygen radicals
- TRITC:
-
tetramethylrhodamine isothyocyanate
References
Baldrian P., Gabriel J.: Intraspecific variability in growth response to cadmium of the wood-rotting fungus Piptoporus betulinus. Mycologia94, 428–436 (2002).
Baldrian P., Gabriel J., Čurdová E., Suchánek M., Rychlovský P.: Heavy and trace elements in wood-inhabiting fungi Fomitopsis pinicola, Ganoderma applanatum, Piptoporus betulinus and Stereum hirsutum from medium polluted sites in Czech Republic. Toxicol.Environ.Chem.71, 475–483 (1999).
Dreyer B., Morte A., Pérez-Gilabert M., Honrubia M.: Autofluorescence detection of arbuscular mycorrhizal fungal structures in palm roots: an underestimated experimental method. Mycol.Res.110, 887–897 (2006).
Gabriel J., Mokrejš M., Bílý J., Rychlovský P.: Accumulation of heavy metals by some wood-rotting fungi. Folia Microbiol.39, 115–118 (1994).
Gabriel J., Vosáhlo J., Baldrian P.: Biosorption of cadmium to mycelial pellets of wood-rotting fungi. Biotechnol.Tech.10, 345–348 (1996a).
Gabriel J., Kofroñová O., Rychlovský P., Krenželok M.: Accumulation and effect of cadmium in the wood-rotting basidiomycete Daedalea quercina. Bull.Environ.Contam.Toxicol.57, 383–390 (1996b).
Galland P., Tölle N.: Light-induced fluorescence changes in Phycomyces: evidence for blue light-receptor associated flavo-semiquinones. Planta217, 971–982 (2003).
Osipowicz B., Jabłoński L, Siewiński A., Jasieńko S., Rymkiewicz A.: Biodegradation of hard coal and its organic extract by selected microorganisms. Fuel73, 1858–1862 (1994).
Rao S., Rajkumar A., Ehtesham M., Prathiba D.: Autofluorescence: a screening test for mycotic infection in tissues. Indian J.Pathol.Microbiol.51, 215–217 (2008).
Samson G., Popovic R.: Use of algal fluorescence for determination of phytotoxicity of heavy metals and pesticides as environmental pollutants. Ecotoxicol.Environ.Saf.16, 272–278 (1988).
Shah V., Verma P., Stopka P., Gabriel J., Baldrian P., Nerud F.: Decolorization of dyes with copper(II)/organic acid/hydrogen peroxide systems. Appl.Catal.B:Environ.46, 287–292 (2003).
Tanabe S., Nishizawa Y., Minami E.: Effects of catalase on the accumulation of H2O2 in rice cells inoculated with rice blast fungus, Magnaporthe oryzae. Physiol.Plant.137, 148–154 (2009).
Valášková V., Baldrian P.: Degradation of cellulose and hemicelluloses by the brown rot fungus Piptoporus betulinus — production of extracellular enzymes and characterization of the major cellulases. Microbiology-SGM152, 3613–3622 (2006).
Valenzuela R., Contreras D., Oviedo C., Freer J., Rodríguez J.: Copper catechol-driven Fenton reactions and their potential role in wood degradation. Internat.Biodeter.Biodegr.61, 345–350 (2008).
Žižka Z., Gabriel J.: Primary fluorescence (autofluorescence) of fruiting bodies of the wood-rotting fungus Fomes fomentarius. Folia Microbiol.51, 109–113 (2006a).
Žižka Z., Gabriel J.: Light-tight boxes for Zeiss photo-micrographic apparatuses. (In Czech) Fine Mechanics and Optics51, 131–133 (2006b).
Žižka Z., Gabriel J.: Autofluorescence of the fruiting body of the fungus Macrolepiota rhacodes. Folia Microbiol.53, 537–539 (2008).
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Žižka, Z., Vêtrovský, T. & Gabriel, J. Enhancement of autofluorescence of the brown-rot fungus Piptoporus betulinus by metal ions. Folia Microbiol 55, 625–628 (2010). https://doi.org/10.1007/s12223-010-0101-y
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DOI: https://doi.org/10.1007/s12223-010-0101-y