Abstract
A gold nanoparticle/reduced graphene oxide composite electrode (AuNP/rGO) was fabricated for the determination of the main bioamines and their precursor amino acids in beer based on a miniaturized capillary electrophoresis with amperometric detection system (mini-CE-AD). The surface morphology and structure of the synthetic AuNP/rGO material were observed by scanning electron microscope, energy dispersive X-ray spectroscope, X-ray diffraction, and Fourier transform infrared spectroscopy. The analytical performance of the composite electrode was verified by cyclic voltammetry and mini-CE-AD approaches. Four target analytes were separated within 7 min in 50 mM Na2B4O7-NaOH buffer (pH 9.35) at a separation voltage of 3 kV. This developed method has been applied to the beer samples, and acceptable recovery could be obtained ranging from 84.5 to 108%. The assay results indicated that this AuNP/rGO composite electrode has the advantages of simple preparation, low working potential (+ 0.65 V vs. SCE), good sensitivity (0.13~0.87 μM), and satisfactory reproducibility. This proposed method provides an alternative for rapid analysis of food safety.
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This work was supported by the Open Funds of the State Key Laboratory of Electroanalytical Chemistry (SKLEAC201801) and the National Students Innovative Experimental Project of China (201810269005G).
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Dan Luo declares that she has no conflict of interest. Xiaoshuang Zhao declares that she has no conflict of interest. Yiliang Zheng declares that she has no conflict of interest. Yaolu Ma declares that she has no conflict of interest. Fei Cun declares that she has no conflict of interest. Jiannong Ye declares that he has no conflict of interest. Qingcui Chu declares that she has no conflict of interest.
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Luo, D., Zhao, X., Zheng, Y. et al. Fast Determination of Main Bioamines and Precursor Amino Acids in Beer by Miniaturized Electrophoresis Using Gold Nanoparticle Composite Electrode. Food Anal. Methods 12, 644–651 (2019). https://doi.org/10.1007/s12161-018-1395-7
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DOI: https://doi.org/10.1007/s12161-018-1395-7