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Disposable amperometric biosensor for the determination of tyramine using plasma amino oxidase

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Abstract

We have developed screen–printed carbon electrodes for the determination of tyramine (Tyr) via plasma amine oxidase. The enzyme was immobilized on the carbon working electrode by cross–linking it with bovine serum albumin using glutaraldehyde. The employment of the mediator hydroxymethylferrocene lowers the working potential to +260 mV (vs. a screen–printed Ag/AgCl reference electrode). The effects of pH, potential and mediator concentration were optimized and resulted in reproducibility and repeatability values of 8.6 % and 8.7 %, respectively. Response is linear in the range from 2 to 164 μM, and the limit of detection is 2.0 ± 0.18 μM. The effects of potentially interfering biogenic amines such as putrescine, cadaverine, histamine, spermine, spermidine and tryptamine were also evaluated. The biosensor was successfully applied to the determination of Tyr in cheese.

Amperometric determination of tyramine using a biosensor based on the immobilization of polyamine oxidase (PAO) enzyme on a disposable screen-printed carbon electrode.

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Acknowledgments

Authors would like to acknowledge funding via Spanish Ministry of Science and Innovation (MICINN) (TEC2009-12029, CENIT-E 2009-INFINITEX, INNPACTO SERIBIO 20011) and Junta de Castilla y León (BU212A12-2). A. Calvo-Pérez is funded by the Junta de Castilla y León and the European Social Found

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001, Burgos, Spain

    Ana Calvo-Pérez, Olga Domínguez-Renedo, María Asunción Alonso-Lomillo & María Julia Arcos-Martínez

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  1. Ana Calvo-Pérez
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  2. Olga Domínguez-Renedo
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  3. María Asunción Alonso-Lomillo
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  4. María Julia Arcos-Martínez
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Correspondence to Olga Domínguez-Renedo.

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Calvo-Pérez, A., Domínguez-Renedo, O., Alonso-Lomillo, M.A. et al. Disposable amperometric biosensor for the determination of tyramine using plasma amino oxidase. Microchim Acta 180, 253–259 (2013). https://doi.org/10.1007/s00604-012-0926-y

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  • DOI: https://doi.org/10.1007/s00604-012-0926-y

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