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Direct analysis of biogenic amines in water matrix by modified capillary zone electrophoresis with 18-crown-6

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Abstract

We report on a method for the determination of the biogenic amines (BAs) spermine, spermidine, histamine, cadaverine, β-phenylethylamine, tyramine and tryptamine. It is based on capillary zone electrophoresis in the presence of 18-crown-6 (180 mM), and is making use of amperometric detection. Under optimized conditions, seven BAs could be well separated within 29 min at a separation voltage of 14 kV in a buffer solution of pH 3.6. The limits of detection for seven BAs are around 10 ng.mL−1 for standard mixtures. The method does not require preconcentration and derivatization steps, and thus provides an attractive alternative to quantitative multi-analysis of BAs in water samples.

Typical electropherogram of the standard mixture solution of seven bioactive amines based on modified capillary zone electrophoresis with 18-crown-6

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Acknowledgements

The authors gratefully acknowledge the National Science Foundation of China (No. 20875032), the Special Funds for the Development of Major Scientific Instruments and Equipment (No. 2011YQ15007205) and the Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. Department of Chemistry, East China Normal University, 3663 North Zhongshan Road, Shanghai, 200062, China

    Wen-li Li, Jin-yuan Ge, Ya-li Pan, Qing-cui Chu & Jian-nong Ye

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  1. Wen-li Li
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  2. Jin-yuan Ge
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  3. Ya-li Pan
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  4. Qing-cui Chu
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  5. Jian-nong Ye
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Correspondence to Qing-cui Chu or Jian-nong Ye.

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Li, Wl., Ge, Jy., Pan, Yl. et al. Direct analysis of biogenic amines in water matrix by modified capillary zone electrophoresis with 18-crown-6. Microchim Acta 177, 75–80 (2012). https://doi.org/10.1007/s00604-011-0755-4

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  • DOI: https://doi.org/10.1007/s00604-011-0755-4

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