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An Overview of Cellulase Immobilization Strategies for Biofuel Production

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Abstract

The use of agricultural residue as a substrate and implementation of nanomaterials for cellulase immobilization can improve the efficiency at higher temperature and will lead to reduce the cost of cellulose-assisted biofuel production. The immobilization utilizing cellulase with amino, chitosan, or polymeric functionalities enhanced stability, activity, reusability, inhibition reduction, purification, and selectivity during enzymatic hydrolysis. Covalent interaction between the substrate and the cellulose marks in greater enzymatic immobilization, which pilots to higher biofuel production. Among the various techniques available for immobilization of cellulase on activated and functionalized magnetic nanoparticles, glutaraldehyde-based covalent binding are the most efficient process for cellulase immobilization. This review provides an overview of different cellulase immobilization strategies, factors, and its kinetics for enhanced biofuel production. The expanding need for low-cost immobilized cellulase, as well as its diverse applications in a variety of industries, is propelling research in this field.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Department of Science and Humanities, Providence College of Engineering, Chengannur, 689 122, Kerala, India

    Reshmy R. & Neena Bachan

  2. Moolec Science, Innovation Centre, Gallows Hill, Warwick, CV34 6UW, UK

    Vivek Narisetty

  3. Division of Livestock Production and Management, ICAR – Indian Veterinary Research Institute, Izatnagar, Bareilly, 243 122, Uttar Pradesh, India

    Ayon Tarafdar

  4. Rajiv Gandhi Center for Biotechnology, Jagathy, Thiruvananthapuram, 695 014, Kerala, India

    Aravind Madhavan

  5. Diatom Research Laboratory, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, 201301, India

    Archana Tiwari

  6. Aquatic Toxicology Laboratory, Environmental Toxicology Group, CSIR-Indian Institute for Toxicology Research (CSIR-IITR), 31 MG Marg, Lucknow, 226001, India

    Preeti Chaturvedi

  7. Gujarat Pollution Control Board, Paryavaran Bhavan, CHH Road, Sector 10 A, Gandhinagar, 382010, Gujarat, India

    Sunita Varjani

  8. Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, 9 Seongbuk-gu, Seoul, 02841, South Korea

    Ranjna Sirohi

  9. Fermentation Technology Division, CSIR-Indian Institute of Integrative Medicine (CSIR-IIIM), Jammu, 180001, J & K, India

    Vinod Kumar

  10. College of Natural Resources and Environment, Northwest A & F University, Yangling, Shaanxi, 712 100, People’s Republic of China

    Mukesh Kumar Awasthi

  11. Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Trivandrum, 695 019, Kerala, India

    Parameswaran Binod

  12. School of Biosciences and Veterinary Medicine, Biosciences and Biotechnology Division, University of Camerino, 62032, Camerino, Italy

    Joseph Amruthraj Nagoth

  13. Department of Food Technology, T K M Institute of Technology, Kollam, 691505, Kerala, India

    Raveendran Sindhu

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Contributions

All authors contributed to the study conception and design. Collecting articles and writing original draft were performed by Reshmy R., Narisetty Vivek, and Ayon Tarafdar. Supervision, reviewing, and editing were done by all the other authors. Raveendran Sindhu performed the project administration and conceptualization. All authors read and approved the final manuscript.

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Correspondence to Raveendran Sindhu.

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Reshmy R., Vivek Narisetty, and Ayon Tarafdar contributed equally and co-first authors.

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R., R., Narisetty, V., Tarafdar, A. et al. An Overview of Cellulase Immobilization Strategies for Biofuel Production. Bioenerg. Res. 16, 4–15 (2023). https://doi.org/10.1007/s12155-022-10431-3

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