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Valorization of Hemicelluloses: Production of Bioxylitol from Poplar Wood Prehydrolyzates by Candida guilliermondii FTI 20037

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Abstract

In this study on the valorization of hemicelluloses (a co-product generated during cellulosic bioethanol production), prehydrolyzates obtained from poplar woodchips pretreated in an industrial experimental steam-explosion pilot-plant facility were evaluated for the production of bioxylitol using the yeast, Candida guilliermondii FTI 20037, employing both batch and fed-batch fermentation modes in shake flasks on defined nutrient medium. The prehydrolyzates consisted of monosaccharides (pentose and hexose sugars) as well as xylo-oligosaccharides and undegraded hemicellulose. Xylose (31.6 ± 0.57 g/L) was the major sugar in the prehydrolyzates that also contained acetic acid and degradation products of lignin and sugars (phenolic and furanic compounds). Xylose in the prehydrolyzates could be further increased (106.4 ± 0.02 g/L) through an acid hydrolysis step (0.6 % (w/v) H2SO4). Compounds of a toxic nature in both the acid hydrolyzates and prehydrolyzates were removed by treatment with Amberlite IRA-400 resin (chloride form). Batch fermentation of pure xylose and poplar prehydrolyzate resulted in bioxylitol production of 9.9 ± 0.01 and 4.9 ± 0.17 g/L, respectively, indicating that the poplar prehydrolyzates exhibited an inhibitory effect on fermentation. After detoxification of the poplar prehydrolyzates, bioxylitol production increased to 8.9 ± 0.01 g/L. Fed-batch fermentation of the prehydrolyzate increased the bioxylitol production to 12.39 ± 0.33 g/L, while acid hydrolysis followed by detoxification resulted in a maximum bioxylitol production of 22.0 ± 0.01 g/L, a 348 % increase. The results demonstrated that acid hydrolysis and detoxification followed by fed-batch fermentation was an efficient way to produce bioxylitol from poplar prehydrolyzates.

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Acknowledgments

The authors gratefully acknowledge the financial support from the Centre for Research and Innovation in the Bioeconomy (CRIBE), Ontario, Canada (RFH Dekker). Dr. Regis Benech, Dr. Stephan Brey, and Greg Santavy of Greenfield Engineering and Technology (Research and Development), Chatham, ON (Canada) are thanked for providing the pilot plant-produced poplar prehydrolyzate samples. The authors gratefully acknowledge the technical expertise of Greg Kepka for assistance with the GC-MS analyses. Yue Sun (summer vacation student) and Magali Ferro (intern; Ecole Nationale Supérieure Agronomique de Toulouse, France) are thanked for their experimental contribution toward this work while at the Biorefining Research Institute during May–August 2013.

Conflict of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario, P7B 5E1, Canada

    Lakmali Nilmini G. Vithanage, Aneli M. Barbosa, Gamini R. Nadu Kankanamge, Sudip K. Rakshit & Robert F. H. Dekker

  2. Biorefining and Biotechnology Consultancy, Rua João Huss 200, Gleba Palhano, CEP: 86050-490, Londrina, PR, Brazil

    Aneli M. Barbosa & Robert F. H. Dekker

  3. School of Engineering, Department of Chemical Engineering, Lakehead University, Thunder Bay, Ontario, Canada, P7B 5E1

    Sudip K. Rakshit

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  1. Lakmali Nilmini G. Vithanage
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Correspondence to Robert F. H. Dekker.

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Vithanage, L.N.G., Barbosa, A.M., Kankanamge, G.R.N. et al. Valorization of Hemicelluloses: Production of Bioxylitol from Poplar Wood Prehydrolyzates by Candida guilliermondii FTI 20037. Bioenerg. Res. 9, 181–197 (2016). https://doi.org/10.1007/s12155-015-9673-3

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