Skip to main content
SpringerLink
Log in

Inhibitory action of toxic compounds present in lignocellulosic hydrolysates on xylose to xylitol bioconversion by Candida guilliermondii

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The inhibitory action of acetic acid, ferulic acid, and syringaldehyde on metabolism of Candida guilliermondii yeast during xylose to xylitol bioconversion was evaluated. Assays were performed in buffered and nonbuffered semidefined medium containing xylose as main sugar (80.0 g/l), supplemented or not with acetic acid (0.8–2.6 g/l), ferulic acid (0.2–0.6 g/l), and/or syringaldehyde (0.3–0.8 g/l), according to a 23 full factorial design. Since only individual effects of the variables were observed, assays were performed in a next step in semidefined medium containing different concentrations of each toxic compound individually, for better understanding of their maximum concentration that can be present in the fermentation medium without affecting yeast metabolism. It was concluded that acetic acid, ferulic acid, and syringaldehyde are compounds that may affect Candida guilliermondii metabolism (mainly cell growth) during bioconversion of xylose to xylitol. Such results are of interest and reveal that complete removal of toxic compounds from the fermentation medium is not necessary to obtain efficient conversion of xylose to xylitol by Candida guilliermondii. Fermentation in buffered medium was also considered as an alternative to overcome the inhibition caused by these toxic compounds, mainly by acetic acid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Cortez DV, Roberto IC (2010) Individual and interaction effects of vanillin and syringaldehyde on the xylitol formation by Candida guilliermondii. Bioresour Technol 101:1858–1865

    Article  CAS  PubMed  Google Scholar 

  2. Delgenes JP, Moletta R, Navarro JM (1996) Effects of lignocellulose degradation products on ethanol fermentations of glucose and xylose by Saccharomyces cerevisiae, Zymomonas mobilis, Pichia stipitis and Candida shehatae. Enzyme Microb Technol 19:220–225

    Article  CAS  Google Scholar 

  3. Duarte LC, Carvalheiro F, Neves I, Girio FM (2005) Effects of aliphatic acids, furfural, and phenolic compounds on Debaryomyces hansenii CCMI 941. Appl Biochem Biotechnol 121:413–425

    Article  PubMed  Google Scholar 

  4. Faria LFF, Gimenes MAP, Nobrega R, Pereira N Jr (2002) Influence of oxygen availability on cell growth and xylitol production by Candida guilliermondii. Appl Biochem Biotechnol 98–100:449–458

    Article  PubMed  Google Scholar 

  5. Helle S, Cameron D, Lam J, White B, Duff S (2003) Effect of inhibitory compounds found in biomass hydrolysates on growth and xylose fermentation by a genetically engineered strain of S. cerevisiae. Enzyme Microb Technol 33:786–792

    Article  CAS  Google Scholar 

  6. Larsson S, Quintana-Sáinz A, Reimann A, Nilvebrant N-O, Jönsson LJ (2000) Influence of lignocellulose-derived aromatic compounds on oxygen-limited growth and ethanolic fermentation by Saccharomyces cerevisiae. Appl Biochem Biotechnol 84–86:617–632

    Article  PubMed  Google Scholar 

  7. Lawford HG, Rousseau JD (1998) Improving fermentation performance of recombinant Zymomonas in acetic acid-containing media. Appl Biochem Biotechnol 70–72:161–172

    Article  Google Scholar 

  8. Luo C, Brink DL, Blanch HW (2002) Identification of potential fermentation inhibitors in conversion of hybrid poplar hydrolyzate to ethanol. Biomass Bioenergy 22:125–138

    Article  CAS  Google Scholar 

  9. Martín C, Galbe M, Nilvebrant N-O, Jönsson LJ (2002) Comparison of the fermentability of enzymatic hydrolyzates of sugarcane bagasse pretreated by steam explosion using different impregnating agents. Appl Biochem Biotechnol 98–100:699–716

    Article  PubMed  Google Scholar 

  10. Mussatto SI, Roberto IC (2002) Xylitol: a sweetener with benefits for human health. Braz J Pharm Sci 38:401–413

    CAS  Google Scholar 

  11. Mussatto SI, Roberto IC (2004) Alternatives for detoxification of diluted acid lignocellulosic hydrolyzates for use in fermentative processes: a review. Bioresour Technol 93:1–10

    Article  CAS  PubMed  Google Scholar 

  12. Mussatto SI, Roberto IC (2005) Evaluation of nutrient supplementation to charcoal-treated and untreated rice straw hydrolysate for xylitol production by Candida guilliermondii. Braz Arch Biol Technol 48:497–502

    Article  Google Scholar 

  13. Palmqvist E, Grage H, Meinander NQ, Hahn-Hägerdal B (1999) Main and interaction effects of acetic acid, furfural, and p-hydroxybenzoic acid on growth and ethanol productivity of yeasts. Biotechnol Bioeng 63:46–55

    Article  CAS  PubMed  Google Scholar 

  14. Parajó JC, Domínguez H, Domínguez JM (1998) Biotechnological production of xylitol. Part 3: operation in culture media made from lignocellulose hydrolysates. Bioresour Technol 66:25–40

    Article  Google Scholar 

  15. Roberto IC, Sato S, Mancilha IM, Taqueda MES (1995) Influence of media composition on xylitol fermentation by Candida guilliermondii using response surface methodology. Biotechnol Lett 17:1223–1228

    Article  CAS  Google Scholar 

  16. Silva DDV, Felipe MGA, Manchilha IM, Luchese RH, Silva SS (2004) Inhibitory effect of acetic acid on bioconversion of xylose in xylitol by Candida guilliermondii in sugarcane bagasse hydrolysate. Braz J Microbiol 35:248–254

    CAS  Google Scholar 

  17. Vogel HC, Todaro CL (1997) Fermentation and biochemical engineering handbook, 2nd edn. Noyes, New Jersey

    Google Scholar 

  18. Zaldivar J, Ingram LO (1999) Effect of organic acids on the growth and fermentation of ethanologenic Escherichia coli LY01. Biotechnol Bioeng 66:203–210

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

Financial support from Capes, Fapesp, and CNPq (Brazil) is gratefully acknowledged.

Author information

Authors and Affiliations

  1. Department of Biotechnology, Engineering College of Lorena, University of São Paulo, Estrada Municipal do Campinho s/n, 12602-810, Lorena, SP, Brazil

    Rogério S. Pereira & Inês C. Roberto

  2. IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    Solange I. Mussatto

Authors
  1. Rogério S. Pereira
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Solange I. Mussatto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Inês C. Roberto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Solange I. Mussatto.

Additional information

This article is part of the BioMicroWorld 2009 Special Issue.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pereira, R.S., Mussatto, S.I. & Roberto, I.C. Inhibitory action of toxic compounds present in lignocellulosic hydrolysates on xylose to xylitol bioconversion by Candida guilliermondii . J Ind Microbiol Biotechnol 38, 71–78 (2011). https://doi.org/10.1007/s10295-010-0830-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10295-010-0830-6

Keywords

Search

Navigation