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Role of Glycerol Addition on Xylose-to-Xylitol Bioconversion by Candida guilliermondii

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Abstract

The effect of glycerol on xylose-to-xylitol bioconversion by Candida guilliermondii was evaluated by its addition (0.7 and 6.5 g/l) to semidefined media (xylose as a substrate). The glycerol concentrations were chosen based on the amounts produced during previous studies on xylitol production by C. guilliermondii. Medium without glycerol addition (control) and medium containing glycerol (53 g/l) in substitution to xylose were also evaluated. According to the results, the addition of 0.7 g/l glycerol to the fermentation medium favored not only the yield (Y P/S = 0.78 g/g) but also the xylitol productivity (Q P = 1.13 g/l/h). During the xylose-to-xylitol bioconversion, the formation of byproducts (glycerol and ethanol) was observed for all conditions employed. In relation to the cellular growth, glycerol as the only carbon source for C. guilliermondii was better than xylose or xylose and glycerol mixtures, resulting in a maximum cellular concentration (5.34 g/l).

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Acknowledgments

The authors gratefully acknowledge the financial support of FAPESP, CAPES, and CNPq. They are also grateful to Lilian Cristina Marton Robin and Rita de Cássia Lacerda Brambilla Rodrigues for reviewing this manuscript.

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  1. Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Estrada Municipal do Campinho s/n, P.O. Box 116, Lorena, SP, 12602-810, Brazil

    Priscila V. Arruda & Maria G. A. Felipe

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  1. Priscila V. Arruda
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  2. Maria G. A. Felipe
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Correspondence to Priscila V. Arruda.

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Arruda, P.V., Felipe, M.G.A. Role of Glycerol Addition on Xylose-to-Xylitol Bioconversion by Candida guilliermondii . Curr Microbiol 58, 274–278 (2009). https://doi.org/10.1007/s00284-008-9321-7

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  • DOI: https://doi.org/10.1007/s00284-008-9321-7

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