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Long noncoding RNA MGC27382 inhibits proliferation and metastasis of non-small cell lung cancer cells via down-regulating AKT/GSK3β pathway

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Abstract

Purpose

Persistent abnormal proliferation and long distant metastasis of tumors contribute to high mortality rate in non-small cell lung cancer (NSCLC) patients. Strategies that prevent NSCLC proliferation and/or metastasis have been studied but still need to be further explored. Numerous studies have proved the diversity functions of long noncoding RNAs (lncRNAs) exerted in cancer, including NSCLC. In this study, we aim to identify and investigate the role of novel lncRNAs in NSCLC progression.

Methods

RNA sequence data were retrieved from the Cancer Genome Atlas (TCGA), differentially expressed lncRNAs (DElncRNAs) were screened out based on the R language, then real-time PCR experiment was introduced to detect the DElncRNA expression levels. A series of experiments including MTT, cell cycle, transwell, and wound healing assays were employed to explore the effect of DElncRNA MGC27382 on cell proliferation and invasion ability.

Results

We detected that DElncRNA MGC27382 is down-regulated in NSCLC tissues and cells. Overexpression of MGC27382 prevented NSCLC cell proliferation via down-regulating cyclin D1 and cyclin E. Moreover, wound healing and transwell assays indicated that the ability of cell invasion and migration could be impaired when cells were treated with MGC27382 overexpression. Further studies demonstrated that MGC27382-mediated inhibition on NSCLC progression can be impaired by LY294002, which is a frequently used inhibitor of AKT/GSK3β pathway.

Conclusion

MGC27382 is down-regulated in NSCLC. It exerts an inhibitory role in NSCLC development through suppressing the AKT/GSK3β pathway. Our results indicate that the lncRNA MGC27382 might be a tumor-suppressor gene in NSCLC. Overexpression of MGC27382 is thought to be a potential strategy for overcoming NSCLC progression.

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Data availability

All data generated or analyzed during this study are included in this published article.

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Acknowledgements

This work was supported by the China Postdoctoral Science Foundation (No. 2018M630786) and the National Natural Science Foundation of China (No. 82002540).

Author information

Authors and Affiliations

  1. Department of Respiratory Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China

    Q. Li

  2. Department of Gastroenterology, People’s Hospital of Weihaiwei, Weihai, China

    S. Li

  3. Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan, China

    L. Niu

  4. Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

    S. Yang

  5. Central Research Laboratory, The Second Hospital of Shandong University, Jinan, China

    H. Niu

  6. Department of Thoracic Surgery, Qilu Hospital, Shandong University, 107 Wenhua Xi Road, Jinan, China

    C. Cheng

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  1. Q. Li
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  4. S. Yang
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  5. H. Niu
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Contributions

QL designed the study and drafted the manuscript. LLN, SLY, HMN, and SL were responsible for the collection and analysis of the experimental data. CCL revised the manuscript critically for important intellectual content. All authors read and approved the final manuscript.

Corresponding author

Correspondence to C. Cheng.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study was approved by the Ethical Committee of Qilu Hospital of Shandong University and conducted in accordance with the ethical standards.

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Yes.

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Li, Q., Li, S., Niu, L. et al. Long noncoding RNA MGC27382 inhibits proliferation and metastasis of non-small cell lung cancer cells via down-regulating AKT/GSK3β pathway. Clin Transl Oncol 23, 2548–2559 (2021). https://doi.org/10.1007/s12094-021-02658-x

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  • DOI: https://doi.org/10.1007/s12094-021-02658-x

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