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Molecular characterization and expression pattern analysis of a novel stress-responsive gene ‘BeSNAC1’ in Bambusa emeiensis

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Abstract

NAC transcription factors (TFs) are master regulators of environmental stresses exerting a crucial role in plant growth and development. However, the studies on NAC TFs from Bambusa emeiensis are scarce. In this investigation, a novel gene from B. emeiensis encoding NAC protein was cloned and characterized. The gene was isolated based on the amino acid sequence data of stress-responsive SNAC1 of rice, named ‘BeSNAC1 (accession no. MG763922)’. The full-length sequence of 1681 bp was found to contain an open-reading frame of 912 bp that encode a protein of 303 amino-acid residues. The multiple protein sequence alignments unveiled that BeSNAC1 contains a typical NAC domain. Additionally, the phylogenetic analysis showed that the corresponding protein belonged to the SNAC group, as it cladded with SNAC1, HvSNAC1, TaNAC2, SbSNAC1 and ZmSNAC1 proteins. Transactivation and subcellular localization assay disclosed that BeSNAC1 is a transcriptional activator localized in the cell nucleus. Moreover, the time-dependent expression pattern of BeSNAC1 was profiled under abscisic acid (ABA), polyethylene glycol 6000 (PEG-6000), NaCl, \(\hbox {H}_{2}\hbox {O}_{2}\) and \(\hbox {Na}_{2}\hbox {SO}_{4}\) treatments via a quantitative real-time polymerase chain reaction. The results revealed that the expression of BeSNAC1 was significantly upregulated in all treatments, a significant difference was observed under \(\hbox {H}_{2}\hbox {O}_{2}\), NaCl and ABA (\(P < 0.001\)) and PEG and \(\hbox {Na}_{2}\hbox {SO}_{4}\) (\(P < 0.01\)) treatments, respectively. Conclusively, our findings provide evidence that ‘BeSNAC1’ is a nuclear protein that might act as part of the transcription regulation complex and is involved in the ABA signalling pathway and abiotic stress tolerance mechanisms in B. emeiensis.

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Acknowledgements

This research was funded by the Department of  Science and Technology,  Sichuan Province, China, project nos. 2016NYZ0038 and 2017NZ0008.

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Authors and Affiliations

  1. Plant Cell Engineering Laboratory, School of Life Science and Engineering, Southwest University of Science and Technology, Main Yang, 621010, Sichuan, People’s Republic of China

    Naseem Samo, Muhammad Imran, Hu Shanglian, Luo Xuegang, Ying Cao & Huang Yan

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  1. Naseem Samo
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  2. Muhammad Imran
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  3. Hu Shanglian
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  4. Luo Xuegang
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  5. Ying Cao
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Correspondence to Hu Shanglian.

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Corresponding editor: Manoj Prasad

HS, conceptualization; NS and MI, investigation; HS, LX and YC, resources; SN and MI, writing and original draft preparation; SN, MI, HS, LX, YC and HY writing, review and editing; HS, supervision; HS and LX, project administration.

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Samo, N., Imran, M., Shanglian, H. et al. Molecular characterization and expression pattern analysis of a novel stress-responsive gene ‘BeSNAC1’ in Bambusa emeiensis. J Genet 98, 52 (2019). https://doi.org/10.1007/s12041-019-1098-x

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